Procyanidin B1 promotes in vitro maturation of pig oocytes by reducing oxidative stress

Jin, Yong-Xun; Hao, Jindong; Huang, Siyi; Wang, Dongxu; Quan, Fu‐Shi; Ren, Wenzhi; Zhang, Jiabao; Zhang, Mingjun; Yu, Xiaofeng

doi:10.1002/mrd.23440

Cited by 11 publications

(13 citation statements)

References 56 publications

(72 reference statements)

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“…As procyanidins increase cell viability, decrease ROS production and restrain oxidative stress in mammalian cells 35, 36 , we next interrogated whether similar or antioxidant effects can be traced in senescent cells exposed to PCC1. Surprisingly, we observed the opposite side, as senescent PSC27 cells displayed elevated ROS levels when treated by PCC1, making a sharp contrast to their proliferating counterparts (Fig.…”

Section: Resultsmentioning

confidence: 99%

Procyanidin C1 is a natural agent with senolytic activity against aging and age-related diseases

et al. 2021

Preprint

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Aging causes functional decline of multiple organs and increases the risk of age-related pathologies. In advanced lives, accumulation of senescent cells, which develop the senescence-associated secretory phenotype (SASP), promotes chronic inflammation and causes diverse conditions. Here we report the frontline outcome of screening a natural product library with human primary stromal cells as an experimental model. Multiple candidate compounds were assayed, and grape seed extract (GSE) was selected for further investigation due to its leading capacity in targeting senescent cells. We found procyanidin C1 (PCC1), a polyphenolic component, plays a critical role in mediating the antiaging effects of GSE. PCC1 blocks the SASP expression when used at low concentrations. Importantly, it selectively kills senescent cells upon application at higher concentrations, mainly by enhancing production of reactive oxygen species (ROS) and disturbing mitochondrial membrane potential, processes accompanied by upregulation of Bcl-2 family pro-apoptotic factors Puma and Noxa in senescent cells. PCC1 depletes senescent cells in treatment-damaged tumor microenvironment (TME) and enhances therapeutic efficacy when combined with chemotherapy in preclinical assays. Intermittent administration of PCC1 to both senescent cell-implanted mice and naturally aged animals alleviated physical dysfunction and prolonged post-treatment survival, thus providing substantial benefits in late life stage. Together, our study identifies PCC1 as a distinct natural senolytic agent, which may be exploited to delay aging and control age-related pathologies in future medicine.

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Section: Resultsmentioning

confidence: 99%

Procyanidin C1 is a natural agent with senolytic activity against aging and age-related diseases

et al. 2021

Preprint

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“…Furthermore, Zhang et al reported that lower doses (5–25 µM) of naturally occurring phenolic compounds (rosmarinic acid) attenuated intracellular ROS levels in porcine oocytes and cumulus cells during IVM [ 59 ]. Moreover, low doses (100 µM) of the polyphenolic compound procyanidin, which is derived from plant sources, also decreased ROS production and apoptosis, while promoting the quality of oocytes and PA embryo development [ 60 ]. Therefore, it is ideal to investigate the anti-oxidative effects of MSmPNPs on the critical stage of IVP in porcine IVM and subsequent embryo development.…”

Section: Discussionmentioning

confidence: 99%

Modified Spirulina maxima Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes

Roy

Qamar

Tanga

et al. 2021

Animals

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Molecular approaches have been used to determine metabolic substrates involved in the early embryonic processes to provide adequate culture conditions. To investigate the effect of modified Spirulina maxima pectin nanoparticles (MSmPNPs) on oocyte developmental competence, cumulus–oocyte complexes (COCs) retrieved from pig slaughterhouse ovaries were subjected to various concentrations of MSmPNPs (0, 2.5, 5.0, and 10 µg/mL) during in vitro maturation (IVM). In comparison to the control, MSmPNPs-5.0, and MSmPNPs-10 groups, oocytes treated with 2.5 µg/mL MSmPNPs had significantly increased glutathione (GSH) levels and lower levels of reactive oxygen species (ROS). Following parthenogenetic activation, the MSmPNPs-2.5 group had a considerably higher maturation and cleavage rates, blastocyst development, total cell number, and ratio of inner cell mass/trophectoderm (ICM:TE) cells, when compared with those in the control and all other treated groups. Furthermore, similar findings were reported for the developmental competence of somatic cell nuclear transfer (SCNT)-derived embryos. Additionally, the relative quantification of POU5F1, DPPA2, and NDP52 mRNA transcript levels were significantly higher in the MSmPNPs-2.5 group than in the control and other treated groups. Taken together, the current findings suggest that MSmPNP treatment alleviates oxidative stress and enhances the developmental competence of porcine in vitro matured oocytes after parthenogenetic activation and SCNT.

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“…Based on the results of previous studies [22,25,29], we can determine that PB1 is a small-molecule drug with antioxidant effects. In addition, we can confirm that it can improve the developmental ability of embryos during early development [29].…”

Section: Discussionmentioning

confidence: 99%

“…Procyanidin B1(PB1) is a small-molecule compound, extracted from a variety of plants [20][21][22][23], especially grapes [24][25][26], and it has strong antioxidant effects [22,24,[27][28][29]. The antioxidant function of PB1 works by reducing the LPS-induced production of ROS and inhibiting extracellular signal-regulated kinase (ERK)1/2 and IkB kinase beta (IKKb) activity [30].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the antioxidant procyanidin B1 contained in grape extracts protects from chronic metabolic diseases such as diabetes and hypertension, and also prevents the development of chronic kidney disease (CKD) and cardiovascular disease [33]. Thus, PB1 has beneficial effects on health and disease due to its antioxidant effects, and PB1 also benefits the in vitro development of PA embryos [29]. So, PB1 may be a potential and beneficial small molecules drug in relation to SCNT embryo development.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Activity and Anti-Apoptotic Effect of the Small Molecule Procyanidin B1 in Early Mouse Embryonic Development Produced by Somatic Cell Nuclear Transfer

Jin

et al. 2021

Molecules

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As an antioxidant, procyanidin B1(PB1) can improve the development of somatic cell nuclear transfer (SCNT) embryos; PB1 reduces the level of oxidative stress (OS) during the in vitro development of SCNT embryos by decreasing the level of reactive oxygen species (ROS) and increasing the level of glutathione (GSH) and mitochondrial membrane potential (MMP). Metabolite hydrogen peroxide (H2O2) produces OS. Catalase (CAT) can degrade hydrogen peroxide so that it produces less toxic water (H2O) and oxygen (O2) in order to reduce the harm caused by H2O2. Therefore, we tested the CAT level in the in vitro development of SCNT embryos; it was found that PB1 can increase the expression of CAT, indicating that PB1 can offset the harm caused by oxidative stress by increasing the level of CAT. Moreover, if H2O2 accumulates excessively, it produces radical-(HO-) through Fe2+/3+ and damage to DNA. The damage caused to the DNA is mainly repaired by the protein encoded by the DNA damage repair gene. Therefore, we tested the expression of the DNA damage repair gene, OGG1. It was found that PB1 can increase the expression of OGG1 and increase the expression of protein. Through the above test, we proved that PB1 can improve the repairability of DNA damage. DNA damage can lead to cell apoptosis; therefore, we also tested the level of apoptosis of blastocysts, and we found that PB1 reduced the level of apoptosis. In summary, our results show that PB1 reduces the accumulation of H2O2 by decreasing the level of OS during the in vitro development of SCNT embryos and improves the repairability of DNA damage to reduce cell apoptosis. Our results have important significance for the improvement of the development of SCNT embryos in vitro and provide important reference significance for diseases that can be treated using SCNT technology.

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Procyanidin B1 promotes in vitro maturation of pig oocytes by reducing oxidative stress

Cited by 11 publications

References 56 publications

Procyanidin C1 is a natural agent with senolytic activity against aging and age-related diseases

Procyanidin C1 is a natural agent with senolytic activity against aging and age-related diseases

Modified Spirulina maxima Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes

Antioxidant Activity and Anti-Apoptotic Effect of the Small Molecule Procyanidin B1 in Early Mouse Embryonic Development Produced by Somatic Cell Nuclear Transfer

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