Procysteine Increases Alcohol-depleted Glutathione Stores in Rat Plantaris Following a Period of Abstinence

Otis, Jeffrey S.; Guidot, D. M.

doi:10.1093/alcalc/agr041

Cited by 4 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More over, it was showed that in fact NAC intake and ethanol abstinence interact synergistically in order to improve the hepatic antioxidant defenses and to decrease the serum lipids concentrations (37). It was also demonstrated relatively recently (38) that procysteine could increase alcohol-depleted glutathione stores in the plantaris muscle of some rat models, after a period of abstinence, considering that chronic alcohol abuse may lead to a variety of skeletal muscle complications, including atrophy, altered gait and impaired mobility (38).…”

Section: Discussionmentioning

confidence: 99%

The Relevance of Oxidative Stress Status in One Week and One Month Alcohol Abstinent Patients

Alexinschi¹,

Chiriță²,

Ciobîcă³

et al. 2014

Journal of Medical Biochemistry

View full text Add to dashboard Cite

SummaryBackground: Although it is generally accepted that there is an increased oxidative stress status in alcoholics, the separate relevance of oxidative stress following alcohol withdrawal is still not understood to this date. There are reports stating that the increased oxidative stress status in alcoholics may persist independently of the constant presence of alcohol intake, while on the other side, it was demonstrated that the antioxidant defense mechanism could significantly increase after alcohol withdrawal. Methods: In the present work, we were interested in studying the relevance of oxidative stress status in the alcohol withdrawal processes, by determining some oxidative stress markers (two antioxidant enzymes: superoxide dismutase -SOD and glutathione peroxidase -GPX and a lipid peroxidation maker -MDA) after one week and one month of abstinence, as compared to the baseline and a control group of subjects. Results: Our data confirmed the increased oxidative stress status in alcoholic patients and, more importantly, we de monstrated here a significant decrease of the oxidative stress status one week and one month following the withdrawal, as showed by a significant increase in the specific activity of

show abstract

Section: Discussionmentioning

confidence: 99%

The Relevance of Oxidative Stress Status in One Week and One Month Alcohol Abstinent Patients

Alexinschi¹,

Chiriță²,

Ciobîcă³

et al. 2014

Journal of Medical Biochemistry

View full text Add to dashboard Cite

show abstract

“…Transverse, serial sections at 10 μ m were cut through the mid‐belly of the TA muscles using a cryostat (Leica CM1850, Leica, Wetzlar, Germany). Sections were then processed for hematoxylin and eosin staining, dehydrated, mounted, and visualized at 10× with a VanGuard light microscope (VEE GEE Scientific, Kirkland, WA, USA) as previously described . Regenerating fibres were identified by the presence of centrally positioned nuclei.…”

Section: Methodsmentioning

confidence: 99%

“…Sections were then processed for hematoxylin and eosin staining, dehydrated, mounted, and visualized at 109 with a VanGuard light microscope (VEE GEE Scientific, Kirkland, WA, USA) as previously described. 4,[53][54][55] Regenerating fibres were identified by the presence of centrally positioned nuclei. Cross-sectional areas (CSA) of approximately 200 regenerated fibres per muscle were calculated using IMAGEJ software (National Institutes of Health, Bethesda, MD, USA).…”

Section: Histologymentioning

confidence: 99%

17‐(allylamino)‐17‐demethoxygeldanamycin drives Hsp70 expression but fails to improve morphological or functional recovery in injured skeletal muscle

Baumann

Otis

2015

Clin Exp Pharma Physio

View full text Add to dashboard Cite

The stress inducible 70 kDa heat shock protein (Hsp70) is instrumental to efficient morphological and functional recovery following skeletal muscle injury because of its roles in protein quality control and molecular signalling. Therefore, in attempt to improve recovery, Hsp70 expression was increased with 17-(allylamino)-17-demethoxygeldanamycin (17-AAG) prior to and following an intramuscular injection of barium chloride (BaCl2) into the tibialis anterior (TA) of healthy young mice. To assess recovery, regenerating fibre cross-sectional area (CSA) of the TA and in vivo peak isometric torque produced by the anterior crural muscles (TA, extensor digitorum longus and extensor hallucis muscles) were analyzed for up to 3 weeks after the injury. Because treatment of 17-AAG and Hsp70 are known to influence inflammatory and myogenic signalling, tumor necrosis factor-α (TNF-α) and myogenin content were also assessed. This study reports that 17-AAG was effective at up-regulating Hsp70 expression, increasing content fivefold in the uninjured muscle. However, this significant increase in Hsp70 content did not enhance morphological or functional recovery following the injury, as the return of regenerating fibre CSA and in vivo peak isometric torque did not differ compared to that of the injured muscle from the vehicle treated mice. Treatment with 17-AAG also altered TNF-α and myogenin content, increasing both to a greater extent after the injury. Together, these findings demonstrate that although 17-AAG may alter molecular makers of regeneration, it does not improve recovery following BaCl2-induced skeletal muscle injury in healthy young mice.

show abstract

“…Glutathione is among the most abundant, soluble, antioxidant molecules in mammalian cells and serves an important role in the anti-oxidation of reactive oxygen species and free radicals (Bray and Taylor 1993;Mytilineou et al 2002). Numerous studies have demonstrated that decreased levels of glutathione are associated with cellular damage (Dam et al 2012;Yamada et al 2011) and alcohol reduces glutathione concentrations (Otis and Guidot 2010;Waly et al 2011) in various tissues.…”

Section: Discussionmentioning

confidence: 99%

Tissue-specific upregulation of HSP72 in mice following short-term administration of alcohol

Islam

Abraham

Hapner

et al. 2013

Cell Stress and Chaperones

View full text Add to dashboard Cite

Oxidative stress and cellular injury have been implicated in induction of HSP72 by alcohol. We investigated the association between HSP72 induction and oxidative stress in mouse tissues following short-term administration of high doses of alcohol and caffeine alone or in combination. Adult male C57BL/6J mice were gavaged with vehicle, alcohol (∼1.7 g/kg/day), caffeine (∼44 mg/kg/day), or alcohol plus caffeine once daily for ten consecutive days. Upon completion of the treatments, tissues were collected for structural and biochemical analyses. Alcohol alone caused mild to moderate lesions in heart, liver, and gastrocnemius muscle. Similar structural changes were observed following administration of alcohol and caffeine combined. Alcohol administration also led to decreased glutathione levels in all three tissues and reduced plasma superoxide dismutase capacity. In contrast, alcohol and caffeine in combination reduced glutathione levels only in liver and gastrocnemius muscle and had no effect on plasma superoxide dismutase. Significant elevations in HSP72 protein and mRNA and in HSF1 protein levels were noted only in liver by alcohol alone or in combination with caffeine. No significant changes in morphology and HSP72 were detected in any tissues tested following administration of caffeine alone. These results suggest that a redox mechanism is involved in the structural impairment caused by short-term high-dose alcohol. Oxidative tissue injury by alcohol may not be associated with tissue HSP72 induction. Induction of HSP72 in liver by alcohol is mediated at both the transcriptional and translational levels.

show abstract

Procysteine Increases Alcohol-depleted Glutathione Stores in Rat Plantaris Following a Period of Abstinence

Cited by 4 publications

References 0 publications

The Relevance of Oxidative Stress Status in One Week and One Month Alcohol Abstinent Patients

The Relevance of Oxidative Stress Status in One Week and One Month Alcohol Abstinent Patients

17‐(allylamino)‐17‐demethoxygeldanamycin drives Hsp70 expression but fails to improve morphological or functional recovery in injured skeletal muscle

Tissue-specific upregulation of HSP72 in mice following short-term administration of alcohol

Contact Info

Product

Resources

About