Produção De Pectina Liase Por Linhagens De Fungos Filamentosos Em Polpas De Buriti E Maracujá Como Fontes De Carbono

Farias, Tiago Nunes; Carvalho, Ilio Fealho de; Machado, Fabiano de Paula Pereira; Sander, Nilo Leal; Silva, Carolina Joana da

doi:10.18677/enciclopedia_biosfera_2015_026

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“…Farias et al (2015) also analyzed the pectinolytic enzymes production. The results showed values greater than 1.40 cm halo degradation pectin at 1.25%.…”

Section: Resultsmentioning

confidence: 99%

Rapid microbiological tests for prospecting new fungal strains with high potentiality for the pectinolytic enzymes production

Cangussu¹,

Felix²,

Araujo³

et al. 2016

Afr. J. Microbiol. Res.

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Prospecting of new isolates producers of pectinolitic enzymes were performed with cultivation of substrates of rice, soybean and corn bran. Pectinolitic enzymes were observed in cultures of the isolates NFC 1, NFC 2, NFC 4, NFC 5 and NFC 6 identified as belonging to the genus Aspergillus sp. Isolates NFR 1, NFR 2 and NFS1 were identified as belonging to the genus Rhizopus and NFC 3 belonging to the genus Cladosporium; however, pectinolitic enzymes production was not observed. The polygalacturonase (PG) production in submerged fermentation of Aspergillus NFC 2 in culture medium proposed (SM) at 28 and 37°C was quantified by agar diffusion and spectrophotometric methods. The highest PG production was obtained at 28°C by greater formation of halo degradation pectin and the activity measured by spectrophotometric methods. The activity of PG obtained was 7 U.mL -1 with volumetric productivity greater in the first 24 h of fermentation both at 28 and 37°C. A decrease in PG synthesis occurred after 48 to 120 h. The study allowed, by means of rapid microbiological tests, the selection of new isolates fungi for PG production and the definition of crucial stages for cultivation in liquid medium.

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“…Farias et al (2015) also analyzed the pectinolytic enzymes production. The results showed values greater than 1.40 cm halo degradation pectin at 1.25%.…”

Section: Resultsmentioning

confidence: 99%