Diabetes is one of the four main noncommunicable diseases and has become one of the fastestgrowing diseases in the world. It will be the seventh leading cause of death worldwide by 2030.The International Diabetes Federation (IDF) has released a new report that demonstrates the global population with diabetes has reached 537 million, increasing 16% since their previous estimation in 2019.Unfortunately, there is no known cure for this chronic health condition; the only alternative to prevent complications for diabetes patients is constantly monitoring their blood glucose levels.Also, the safe range for blood glucose levels is very narrow (between 80-120 mg/dl). Therefore, the sensing method should be able to detect a slight change in concentration in this range. In order to measure the glucose level, biosensors are very practical. A biosensor is transducer capable of converting a biological response into a measurable signal.A novel, low-cost, sensitive microwave microfluidic glucose detecting biosensor incorporating molecularly imprinted polymer is presented. The sensing device is based on dual stub resonators to characterize water glucose solutions. The tip of one of the stubs is coated with MIP to increase the selectivity of the sensor and hence the sensitivity compared to the uncoated or non-imprinted polymer one. The sensor was fabricated on an FR4 substrate for low-cost purposes.In the presence of the MIP, the sensor load-ed with a glucose solution ranging from 50 mg/dL to 400 mg/dL was observed to experience an absorption frequency shift of 73 MHz when the solutions flow in a microfluidic channel passing sensing area, while the Lower Limit of Detection (LLD) of the sensor was discovered to be 2.4 ng/dL. The experimental results showed a high sensitivity of 1.3 MHz /(mg/dL) in terms of absorption frequency.