Produção e degradação in vitro de estruturas tubulares de celulose bacteriana

Oliveira, Vanessa A.; Rambo, Carlos R.; Porto, Luismar Marques

doi:10.4322/polimeros.2013.041

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…The ability of bacterial cellulose to be degraded has not yet been fully elucidated. In animal and human tissues, it is considered limited due to the absence of hydrolases that rupture the ß (1,4) binding of the cellulose chain, which is responsible for the solubility of the biomaterial ( Oliveira, Rambo & Porto, 2013 ). Although the idea of a completely degradable scaffold is interesting from the point of view of tissue engineering, there remain difficulties with materials that exhibit this property, since the timing of degradation and tissue repair combined with the mechanical properties acquired by the newly formed tissue have led researchers to believe that a material with a low rate of degradation may respond better when the scarring process requires more time-consuming conditions ( Bhattacharjee et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Behavior and biocompatibility of rabbit bone marrow mesenchymal stem cells with bacterial cellulose membrane

Silva

Leite

Carvalho

et al. 2018

PeerJ

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BackgroundTissue engineering has been shown to exhibit great potential for the creation of biomaterials capable of developing into functional tissues. Cellular expansion and integration depends on the quality and surface-determinant factors of the scaffold, which are required for successful biological implants. The objective of this research was to characterize and evaluate the in vitro characteristics of rabbit bone marrow mesenchymal stem cells (BM-MSCs) associated with a bacterial cellulose membrane (BCM). We assessed the adhesion, expansion, and integration of the biomaterial as well as its ability to induce macrophage activation. Finally, we evaluated the cytotoxicity and toxicity of the BCM.MethodsSamples of rabbit bone marrow were collected. Mesenchymal stem cells were isolated from medullary aspirates to establish fibroblast colony-forming unit assay. Osteogenic, chondrogenic, and adipogenic differentiation was performed. Integration with the BCM was assessed by scanning electron microscopy at 1, 7, and 14 days. Cytotoxicity was assessed via the production of nitric oxide, and BCM toxicity was assessed with the MTT assay; phagocytic activity was also determined.ResultsThe fibroblastoid colony-forming unit (CFU-F) assay showed cells with a fibroblastoid morphology organized into colonies, and distributed across the culture area surface. In the growth curve, two distinct phases, lag and log phase, were observed at 15 days. Multipotentiality of the cells was evident after induction of osteogenic, chondrogenic, and adipogenic lineages. Regarding the BM-MSCs’ bioelectrical integration with the BCM, BM-MSCs were anchored in the BCM in the first 24 h. On day 7 of culture, the cytoplasm was scattered, and on day 14, the cells were fully integrated with the biomaterial. We also observed significant macrophage activation; analysis of the MTT assay and the concentration of nitric oxide revealed no cytotoxicity of the biomaterial.ConclusionThe BCM allowed the expansion and biointegration of bone marrow progenitor cells with a stable cytotoxic profile, thus presenting itself as a biomaterial with potential for tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Behavior and biocompatibility of rabbit bone marrow mesenchymal stem cells with bacterial cellulose membrane

Silva

Leite

Carvalho

et al. 2018

PeerJ

View full text Add to dashboard Cite

show abstract

“…The ability of bacterial cellulose to be degraded has not yet been fully elucidated. In animal and human tissues, it is considered limited due to the absence of hydrolases that rupture the ß (1,4) binding of the cellulose chain, which is responsible for the solubility of the biomaterial (Oliveira, Rambo & Porto, 2013). Manuscript to be reviewed mechanical properties acquired by the newly formed tissue have led researchers to believe that a material with a low rate of degradation may respond better when the scarring process requires more time-consuming conditions (Bhattacharjee et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Behavior and biocompatibility of rabbit bone marrow mesenchymal stem cells with bacterial cellulose membrane (v0.1)"

2018

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Background. Tissue engineering has been shown to exhibit great potential for the creation of biomaterials capable of developing into functional tissues. Cellular expansion and integration depends on the quality and surface-determinant factors of the scaffold, which are required for successful biological implants. The objective of this research was to characterize and evaluate the in vitro characteristics of rabbit bone marrow mesenchymal stem cells (BM-MSCs) associated with a bacterial cellulose membrane (BCM). We assessed the adhesion, expansion, and integration of the biomaterial as well as its ability to induce macrophage activation. Finally, we evaluated the cytotoxicity and toxicity of the BCM. Methods. Samples of rabbit bone marrow were collected. Mesenchymal stem cells were isolated from medullary aspirates to establish fibroblast colony-forming unit assay. Osteogenic, chondrogenic, and adipogenic differentiation was performed. Integration with the BCM was assessed by scanning electron microscopy at 1, 7, and 14 days. Cytotoxicity was assessed via the production of nitric oxide, and BCM toxicity was assessed with the MTT assay; phagocytic activity was also determined. Results. The fibroblastoid colonyforming unit (CFU-F) assay showed cells with a fibroblastoid morphology organized into colonies, and distributed across the culture area surface. In the growth curve, two distinct phases, lag and log phase, were observed at 15 days. Multipotentiality of the cells was evident after induction of osteogenic, chondrogenic, and adipogenic lineages. Regarding the BM-MSCs' bioelectrical integration with the BCM, BM-MSCs were anchored in the BCM in the first 24 h. On day 7 of culture, the cytoplasm was scattered, and on day 14, the cells were fully integrated with the biomaterial. We also observed significant macrophage activation; analysis of the MTT assay and the concentration of nitric oxide revealed no cytotoxicity of the biomaterial. Conclusion. The BCM allowed the expansion and

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“…Tissue engineering approaches are being used to develop palliative methods for these pathologies such as the construction of artificial blood vessels. The purpose of this study was to biosynthesize artificial vessels using Gluconacetobacter hasenii 's bacterial cellulose (BC) as scaffolding [ 3 ]. Functional and structural characteristics of the vessels were evaluated, as well as the coating of the cellulolytic tubular scaffolding with human aortic smooth muscle cells (HASMC).…”

Section: Introductionmentioning

confidence: 99%