Producing alcohol and salt stress tolerant strain of Saccharomyces cerevisiae by heterologous expression of pprI gene

Helalat, Seyed Hossein; Bidaj, Shirin; Samani, Sadaf; Moradi, Mohammad

doi:10.1016/j.enzmictec.2019.01.008

Cited by 21 publications

(15 citation statements)

References 19 publications

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“…In the fermentation process, carbon sources from organic wastes are preferred over chemical substrates (pure glucose) due to their lower costs [5][6][7][8]14]. In this study, optimal conditions for the enzymatic hydrolysis of biomass, which can be used as a carbon source, were investigated to hydrolyze the substrates in high yields.…”

Section: Selection Of Enzyme Concentrationmentioning

confidence: 99%

“…These food wastes affect public health as well as the environment because they are disposed of by landfilling or incineration [4,5]. Sustainable development has been remarked to deal with environmental concerns and economic growth in the long term [6][7][8]. To this end, a biorefinery system has received considerable attention, as it is a continuous eco-friendly process that uses renewable biomass, such as food waste and microalgae [9,10].…”

Section: Introductionmentioning

confidence: 99%

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Improved Sugar Recovery from Orange Peel by Statistical Optimization of Thermo-Alkaline Pretreatment

2021

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Orange peel, which is a by-product of oranges, contains carbohydrates that can be converted into sugars and used in the fermentation process. In this study, the thermal alkaline pretreatment process was chosen because of its simplicity and lesser reaction time. In addition, the reaction factors were optimized using response surface methodology. The determined optimal conditions were as follows: 60.1 g/L orange peels loading, 3% KOH and 30 min. Under the optimal conditions, glucan content (GC) and enzymatic digestibility (ED) were found to be 32.8% and 87.8%, respectively. Enzymatic hydrolysis was performed with pretreated and non-pretreated orange peels using three types of enzyme complex (cellulase, cellobiase and xylanase). The minimum concentrations of enzyme complex required to obtain maximum ED were 30 FPU (filter paper unit), 15 CBU (cellobiase unit), and 30 XNU (xylanase unit) based on 1 g-biomass. Additionally, ED of the treated group was approximately 3.7-fold higher than that of the control group. In conclusion, the use of orange peel as a feedstock for biorefinery can be a strategic solution to reduce wastage of resources and produce sustainable bioproducts.

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Section: Selection Of Enzyme Concentrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improved Sugar Recovery from Orange Peel by Statistical Optimization of Thermo-Alkaline Pretreatment

2021

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“…Fermented microbes can still survive in such ethanol concentrations. At 7.5 percent ethanol levels, normal cell growth stopped, while the mutant strain tolerated up to 11 percent ethanol [26]. All voltages and longer treatment time in the electrodialysis process had a significant influence on the levels of ethanol.…”

Section: Fermentationmentioning

confidence: 97%

Desalination Technique on Seaweeds Hydrolysate Eucheuma Cottonii for Bioethanol Production

Fakhrudin¹,

Setyaningsih²,

Rahayuningsih³

2021

Int. J. Eng. Scie. and Inform. Technology.

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The dissolved salts on Eucheuma cottonii hydrolysate interfere the growth of S. cerevisiae in the fermentation as it is considered as inhibitors. These salts are derived from biomass and formed from the chemicals used for hydrolysis processes such as H2SO4 and CaOH2. Ions and cations of the salts are potential as inhibitors such as Na+, Cl-, NH4+, SO42-. Osmotic pressure is raised due to the presence of salt. The efforts had been made to reduce salinity level through electrodialysis. The objective of this study was to eliminate dissolved salts in the hydrolysate in order to optimize the process of fermentation and increase ethanol yield. The results showed that the process of desalination by electrodialysis was able to reduce the salinity of 20% on the voltage of 5 V for 30 minutes; the voltage 9 V for 15 minutes; the voltage 12 V for 30 minutes. The lowest decline of SO42- occurred at treatment of 12 V for 45 minutes. The content of sulfate in the treatment decreased to 2.97 g/l or 46.22%. The more sulfate is reduced through the electrodialysis process, the better the fermentation process is carried out. Desalination treatment at a voltage of 5 V for 30 minutes had been the best treatment because it produced the highest ethanol yield of 2.06%. All the voltage treatments and the length of time on the electrodialysis process had a significant influence on the levels of ethanol yield. The process of desalination by electrodialysis was able to reduce the levels of dissolved salts, then it had a significant effect on ethanol yield.

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“…Application of laccase from Yarrowia lipolytica removed phenolic inhibitors in vivo during rice straw hydrolysis . More recently, the pprI gene from Deinococcus radiodurans was overexpressed in S. cerevisiae CEN.PK 113‐5D, which significantly improved not only salt, ethanol, and butanol tolerance, but also increased ethanol production from wheat bran and straw hydrolysates …”

Section: Expanding S Cerevisiae Inhibitor Resistancementioning

confidence: 99%

Overcoming lignocellulose‐derived microbial inhibitors: advancing the Saccharomyces cerevisiae resistance toolbox

Brandt

Jansen

Görgens

et al. 2019

Biofuels Bioprod Bioref

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Lignocellulose-derived biofuels present an attractive carbon-neutral alternative to fossil fuels amidst growing global energy and climate concerns. Bioethanol in particular, has been shown to be a viable additive to and / or replacement for petroleum in countries such as Brazil. The bioconversion of lignocellulose biomass to bioethanol is a developing technology with the yeast Saccharomyces cerevisiae playing a pivotal role in the fermentation-based processes. This yeast however, is challenged to ferment under harsh conditions, specifically, during exposure to lignocellulose-derived microbial inhibitors that are formed during pretreatment processes. This detrimentally affects biocatalyst performance, ultimately decreasing ethanol productivity and yield. To this end, S. cerevisiae strains are in development to increase the yeast microbial inhibitor resistance towards cost-effective cellulosic bioethanol production. This review discusses the current status of inhibitor resistance in S. cerevisiae strains and perspectives on the future of multi-tolerant phenotypes with a specific focus on existing and emerging strain development strategies designed to improve resistance phenotypes.

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Producing alcohol and salt stress tolerant strain of Saccharomyces cerevisiae by heterologous expression of pprI gene

Cited by 21 publications

References 19 publications

Improved Sugar Recovery from Orange Peel by Statistical Optimization of Thermo-Alkaline Pretreatment

Improved Sugar Recovery from Orange Peel by Statistical Optimization of Thermo-Alkaline Pretreatment

Desalination Technique on Seaweeds Hydrolysate Eucheuma Cottonii for Bioethanol Production

Overcoming lignocellulose‐derived microbial inhibitors: advancing the Saccharomyces cerevisiae resistance toolbox

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