Background, aim, and scope Packaging uses nearly 40% of all polymers, a substantial share of which is used for sensitive merchandise such as moisture-sensitive food. To find out if bio-based materials are environmentally advantageous for this demanding application, we compared laminated, printed film across the whole life cycle. Materials and methods We compared bio-based materials (paper, polylactic acid, bio-based polyethylene, and a biobased polyester) as well as conventional ones (polypropylene, polyethylene). Data stemmed from 13 companies that produce raw materials, films and/or laminates and which co-operated with us in a project commissioned by a large food producer. The functional unit chosen for this study is 1 m 2 of packaging film. This is (mostly) laminated, printed film that is delivered on reels to the food industry, where the laminate is cut, sealed and filled. The impact assessment is presented for non-renewable energy use, total energy use, global warming potential, depletion of abiotic resources, photo-oxidant formation, acidification, eutrophication, water use, and land use.