Producing pasture-like milk from goats in confinement

Hadaya, Oren; Landau, S.; Glasser, Tzach; Muklada, H.; Deutch, Tova; Shemesh, Moshe; Argov-Argaman, Nurit

doi:10.1016/j.livsci.2020.104056

Cited by 7 publications

(11 citation statements)

References 33 publications

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“…In particular, enhanced lipid production was concomitant with increased diameter of intracellular lipid droplets (the milk fat globule precursors); casein secretion was elevated; lactose synthesis and secretion was enhanced, and mitochondrial activity was more resilient to pro-oxidant load, as expressed by antioxidant activity, coupling efficiency and spare respiration capacity. In farm animals, the utilization of exogenous antioxidants was studied in several aspects: improvement of milk components and quality for human health 29,30 , vascular homeostasis during prooxidant challenge 31 , incidence of mastitis 32 semen quality 33 , plasma redox status 34 and weight gain of bull calves 35 . However, no cellular mechanism was investigated for these production traits till now.…”

Section: Discussionmentioning

confidence: 99%

“…In farm animals, the utilization of exogenous antioxidants was studied in several aspects: improvement of milk components and quality for human health 29 , 30 , vascular homeostasis during prooxidant challenge 31 , incidence of mastitis 32 semen quality 33 , plasma redox status 34 and weight gain of bull calves 35 . However, no cellular mechanism was investigated for these production traits till now.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of polyphenols and flavonoids in the hydrolysed and non-hydrolysed solutions was determined using reversed-phase HPLC analysis with binary gradient elution on a Thermo Scientific Finnigan Surveyor system equipped with a PDA Plus Detector (220-450 nm) (Thermo Fisher Scientific, Waltham, MA, USA). As recently published by Hadaya et al 29 Lipid extraction and analysis. After the 24-h treatment with PLEE, total lipids were extracted from harvested cells with trypsin (0.05%), washed with phosphate buffered saline (PBS) and stored at − 20 °C until lipid extraction as previously described 62 .…”

Section: Methodsmentioning

confidence: 99%

“…The presence of polyphenols and flavonoids in the hydrolysed and non-hydrolysed solutions was determined using reversed-phase HPLC analysis with binary gradient elution on a Thermo Scientific Finnigan Surveyor system equipped with a PDA Plus Detector (220–450 nm) (Thermo Fisher Scientific, Waltham, MA, USA). As recently published by Hadaya et al 29 , the chromatographic separation was performed on a Gemini 5µ C6-Phenyl 110 Å column (250 × 4.60 mm) (Phenomenex, Torrance, CA, USA) at 30 °C. The mobile phase consisted of 0.1% acetic acid in water (A) and 0.1% acetic acid in methanol (B), and the run was programmed for 35 min.…”

Section: Methodsmentioning

confidence: 99%

“…In dairy goats provided with P. lentiscus as a major source of forage, milk production and quality were much improved 26 , 27 . While some effects of P. lentiscus , such as elevated protein concentration in milk, can be explained by the polyphenol effect on rumen fermentation, digestion and absorption of proteins 28 , others, such as higher milk-fat production and phospholipid concentration in milk are most likely due to systemic effects of the Pistacia polyphenols 29 . Although lentisk has been widely investigated due to its mitigating effects on inflammation, to the best of our knowledge, the antioxidant role of P. lentiscus in mammary cell production traits has never been studied.…”

Section: Introductionmentioning

confidence: 99%

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Pistacia lentiscus extract enhances mammary epithelial cells’ productivity by modulating their oxidative status

Hadaya

Bransi-Nicola

Shalev

et al. 2020

Sci Rep

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We assessed the potential of phenolic compounds from Pistacia lentiscus (lentisk) to enhance production of milk constituents in bovine mammary epithelial cells (MEC). MEC were exposed to 0 (control), 1 or 10 ppm of polyphenols from lentisk ethanolic extract (PLEE) for 24 h. PLEE were absorbed by the MEC plasma membrane, but also penetrated the cell to accumulate in and around the nucleus. PLEE increased triglyceride content in the cell and its secretion to the medium, and significantly increased intracellular lipid droplet diameter. Compared to control, PLEE increased dose-dependently the lactose synthesis, secretion of whey proteins, and contents of casein. To evaluate mitochondrial activity under pro-oxidant load, MEC were preincubated with PLEE and exposed for 2 h to H2O2. Exposure to H2O2 increased the proportion of cells with impaired mitochondrial membrane potential twofold in controls, but not in PLEE-pre-treated cells. Accordingly, proton leakage was markedly decreased by PLEE, and coupling efficiency between the respiratory chain and ATP production was significantly enhanced. Thus, lentisk polyphenols divert energy to production of milk fat, protein and lactose, with less energy directed to cellular damage control; alternatively, PLEE enables MEC to maintain energy and oxidative status under extreme metabolic rate required for milk production and secretion, and reduces the limitation on energy required to support production.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The presence of polyphenols and flavonoids in the hydrolysed and non-hydrolysed solutions was determined using reversed-phase HPLC analysis with binary gradient elution on a Thermo Scientific Finnigan Surveyor system equipped with a PDA Plus Detector (220–450 nm) (Thermo Fisher Scientific, Waltham, MA, USA). As recently published by Hadaya et al 29 , the chromatographic separation was performed on a Gemini 5µ C6-Phenyl 110 Å column (250 × 4.60 mm) (Phenomenex, Torrance, CA, USA) at 30 °C. The mobile phase consisted of 0.1% acetic acid in water (A) and 0.1% acetic acid in methanol (B), and the run was programmed for 35 min.…”

Section: Methodsmentioning

confidence: 99%