Product evaluation: blood compatibility studies

Bhatt, Anugya; Nair, Renjith P.; Ramakrishnan, Rashmi; Raju, Riya; Geeverghese, Rency; Lekshmi, P

doi:10.1016/b978-0-12-823966-7.00022-0

Cited by 3 publications

(5 citation statements)

References 57 publications

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“…Blood–biomaterials interactions are dependent on various pathological/health biological parameters, and also are strongly connected with the surface properties and the end application of the medical devices [ 44 ]. Blood contains cells (platelets, red cells, and white cells) and plasma; the liquid phase is abundant in proteins and other small molecules, and the surface of the biomaterial interacts with these components.…”

Section: Resultsmentioning

confidence: 99%

Xanthan-Based Materials as a Platform for Heparin Delivery

et al. 2023

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Heparin (Hep), with its anticoagulant activity, antiangiogenic and apoptotic effects, and growth factor binding, plays an important role in various biological processes. Formulations as drug delivery systems protect its biological activity, and limit the potential side effects of faulty administration. The objective of this study was to develop novel xanthan-based materials as a delivery carrier for heparin. The materials exhibited remarkable elastic behavior and toughness without any crack development within the network, which also support their application for tissue engineering. It was found that all materials possessed the ability to control the release of heparin, according to the Korsmeyer–Peppas release model. All Hep-containing materials caused significant exchanges of the activated partial thromboplastin time (aPTT) and prothrombin time (PT) parameters, indicating that formulated natural/natural synthetic polymeric networks conserved heparin’s biological activity and its ability to interrupt the blood coagulation cascade. The obtained results confirmed that developed materials could be carriers for the controlled release of heparin, with potential applications in topical administration.

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Section: Resultsmentioning

confidence: 99%

Xanthan-Based Materials as a Platform for Heparin Delivery

et al. 2023

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“…There are physicochemical properties, which estimate biocompatibility of hydrogel, relating to cell adhesion, and proliferation. 93,94 Previous studies of the bacterial surface have reported the hydrophobic properties of bacteria, due to components of bacterial structure such as peptidoglycan, lipopolysaccharide, and formation of polysaccharide biofilm by EPS. 1,95,96 Therefore, bacteria can attach on both hydrophobic and hydrophilic surfaces, depending on the component of cell membrane structures.…”

Section: Impact Of Wettability and Extracellular Polymeric Substancesmentioning

confidence: 99%

“…Wettability is described as hydrophobic and hydrophilic properties of surface materials. There are physicochemical properties, which estimate biocompatibility of hydrogel, relating to cell adhesion, and proliferation 93,94 . Previous studies of the bacterial surface have reported the hydrophobic properties of bacteria, due to components of bacterial structure such as peptidoglycan, lipopolysaccharide, and formation of polysaccharide biofilm by EPS 1,95,96 .…”

Section: Impact Of Wettability and Extracellular Polymeric Substancesmentioning

confidence: 99%

Impact of polymeric films and hydrogels: Physical characteristics on bacterial growth

Techakasikornpanich,

Jangpatarapongsa,

Polpanich

et al. 2024

Polymers for Advanced Techs

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Hydrogels find diverse applications in manipulating bacteria, and serving purposes like elevation, maintenance, and elimination. Several factors of hydrogel have been studied in the benefits of antibacterial activity. Factors such as hydrogel stiffness and roughness gain significance in surface coating, influencing bacterial behavior. However, the intricate interplay of hydrogel stiffness, roughness, polymer types, and bacterial species necessitates further exploration. The choice of polymer is dictated by the specific objectives, particularly in antibacterial scenarios where polymers with positive charge, hydrophilicity, and acidity prove effective. These properties induce robust electrostatic and hydrophobic/hydrophilic interactions, along with pH‐induced cell membrane damage, collectively contributing to hindered bacterial adhesion and growth. Additionally, extracellular polymeric substances (EPS) emerge as pivotal influencers in bacterial adhesion and proliferation. EPS production alters bacterial surfaces, fostering connections between bacteria and facilitating biofilm formation. The hydrophobic nature of EPS further complicates bacterial interactions with surface materials, emphasizing the nuanced interplay of hydrophilic and hydrophobic forces in bacterial adhesion. Herein, this work article has reviewed the related study of each physical property related to antibacterial property on the surface of the hydrogel. Moreover, this work also illustrates applications of the antibacterial properties of hydrogel for medical and surface treatment, including wound healing, food packaging, and surface coating. Additionally, the bacteria growing on hydrogel for engineered living materials, have been updated in various applications.

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“…Biological tests are considered to be safe alternative assays that mimic cell interactions in vitro when examining the biological properties of a treatment due to their “natural” status [ 15 ]. Hemocompatibility evaluates the compatibility of plain materials or loaded materials with drugs, proteins, oils, etc., with blood [ 15 , 16 ]. In detail, hemolysis determines the degree of red cell lysis and the release of hemoglobin [ 15 , 16 , 17 ] caused by materials or their extracts in vitro.…”

Section: Introductionmentioning

confidence: 99%

“…Hemocompatibility evaluates the compatibility of plain materials or loaded materials with drugs, proteins, oils, etc., with blood [ 15 , 16 ]. In detail, hemolysis determines the degree of red cell lysis and the release of hemoglobin [ 15 , 16 , 17 ] caused by materials or their extracts in vitro. Moreover, reactive oxygen species (ROS) are frequently produced by aquatic organisms in reaction to stressful factors [ 18 ], which has an impact on the quality of the end bio-product.…”

Section: Introductionmentioning

confidence: 99%

Oil from Mullet Roe Byproducts: Effect of Oil Extraction Method on Human Erythrocytes and Platelets

Tsamesidis,

Tzika,

Georgiou

et al. 2023

Foods

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Background: The valorization of byproducts to obtain high nutritional value foods is of utmost importance for our planet where the population is booming. Among these products are oils rich in ω-3 fatty acids produced from fishery byproducts. Recently, mullet roe oil from roe byproducts was produced that was rich in the ω-3 fatty acids eicosatetraenoic acid (EPA) and docosahexaenoic acid (DHA). Oils are customarily characterized for their composition and degree of oxidation but little is known of their biological effects, especially the effect of the extraction method. Methods: The purpose of this study was to evaluate the effects of freshly extracted mullet roe oil from mullet roe byproducts and the effect of the extraction method on human red blood cells (hRBCs) and platelets. To this end, the hemocompatibility (cytotoxicity), oxidative effects, and erythrocyte membrane changes were examined after 1 and 24 h of incubation. Antiplatelet effects were also assessed in vitro. Results: The expeller press oil extraction method and alcalase-assisted extraction produced the most biocompatible oils, as shown by hemocompatibility measurements and the absence of erythrocyte membrane alterations. Solvent extracts and protease-assisted extraction oils resulted in the rupture of red blood cells at different examined dilutions, creating hemolysis. Conclusions: It seems that the proper functioning of oil–erythrocyte interactions cannot be explained solely by ROS. Further investigations combining chemical analysis with oil–cell interactions could be used as an input to design high nutritional value oils using green extraction technologies. All samples exhibited promising antiplatelet and antiblood clotting effects in vitro.

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Product evaluation: blood compatibility studies

Cited by 3 publications

References 57 publications

Xanthan-Based Materials as a Platform for Heparin Delivery

Xanthan-Based Materials as a Platform for Heparin Delivery

Impact of polymeric films and hydrogels: Physical characteristics on bacterial growth

Oil from Mullet Roe Byproducts: Effect of Oil Extraction Method on Human Erythrocytes and Platelets

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