Product of
            <i>Staphylococcus aureus</i>
            Responsible for the Scalded-Skin Syndrome

Kapral, Frank A.; Miller, Margaret

doi:10.1128/iai.4.5.541-545.1971

Cited by 152 publications

(72 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, reducing the total volume of bacterial cell culture supernatant on the surface of the dialysis sac to only 2^3 ml per £ask (compared to previously reported volumes of up to 50 l [11,14,16,17]) produced a very concentrated crude toxin preparation, which was easier to handle. Two main puri¢cation procedures for the toxins have been described previously^one based on ion exchange chromatography [7,11,12] and the other on isoelectric focusing [8,15^17]. The DEAE^ion exchange was preferred in this case because it removed a larger number of contaminating proteins.…”

Section: Discussionmentioning

confidence: 99%

A novel method for rapid production and purification of exfoliative toxin A of Staphylococcus aureus

Ladhani

2002

FEMS Microbiology Letters

View full text Add to dashboard Cite

The exfoliative toxins of Staphylococcus aureus are the causative agents of the scalded-skin syndrome. Previously described methods of toxin production and purification require large quantities of culture medium, take a long time and often produce low yields of toxin. A novel method of toxin production and purification using a dialysis sac to separate the culture medium from the staphylococci is described. This method produces up to 12 mg of crude toxin per ml of bacterial cell culture bathing the surface of the dialysis sac within 36 h and almost 10 mg of purified toxin per ml of cell culture within 3 days, in contrast to previous procedures that took over a week to produce 0.1^1.0 mg ml 31 crude toxin and less than 0.01 mg ml 31 purified toxin. This rapid method of toxin production should speed up future research into the pathogenesis of the staphylococcal scalded-skin syndrome. ß

show abstract

Section: Discussionmentioning

confidence: 99%

A novel method for rapid production and purification of exfoliative toxin A of Staphylococcus aureus

Ladhani

2002

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…The isolation of the toxic factor now known as epidermolytic toxin followed soon thereafter (Arbuthnott et al 1971(Arbuthnott et al , 1972Kapral & Miller 1971. The toxin has a molecular weight of approximately 25000 and the main form has an isoelectric point of 7-0 (Arbuthnott et al 1974).…”

Section: Syndromementioning

confidence: 99%

Guest Lecture*

1978

Journal of Applied Bacteriology

View full text Add to dashboard Cite

“…Since Melish & Glasgow (1) demonstrated that staphylococcal scalded skin syndrome (SSSS) could be reproduced in newborn mice by injecting staphylococci cultured from a SSSS patient, it has been well established that intraepidermal splitting is caused by the action of staphylococcal exfoliative toxin (ET) (2)(3)(4). However, no precise mechanism of action has been elucidated.…”

Section: Introductionmentioning

confidence: 99%

Partial Purification and Some Characteristics of Proteinase(s) Induced by Staphylococcal Exfoliative Toxin

Suzuki

Teramoto

Tazawa

et al. 1989

The Journal of Dermatology

View full text Add to dashboard Cite

After 8 hrs incubation with epidermis of newborn mice and exfoliative toxin, a marked increase in caseinolytic activity was detected, which reached a maximum at 12 hrs. Casein-hydrolyzing enzyme(s) induced by ET were partially purified by chromatography. A substantial increase in caseinolytic activity was detected in the fractions obtained from Sephadex G-50, while practically no caseinolytic activity was observed when the extract obtained from the epidermis incubated without ET was applied. The caseinolytic activity appeared as a single peak eluted from DEAE-Sepharose CL-6B and Sephadex G-75. However, on SDS-PAGE, the partially purified fractions exhibited several protein bands. The molecular weights of these band were estimated as 78 KD, 68 KD, 45 KD, 14.5 KD and 8.8 KD. When the partially purified enzyme(s) was preincubated with EGTA or EDTA, substantial inhibition of the activity was observed; however, no recovery of the activity was detected after the addition of CaCl2. Treatment of the enzyme(s) with PMSF and NEM caused little inactivation of the activity. Enzyme activity retained about 57% of the initial activity following 3 min incubation at 60 degrees C, but was completely inactivated after 4 min.

show abstract

Product of Staphylococcus aureus Responsible for the Scalded-Skin Syndrome

Cited by 152 publications

References 9 publications

A novel method for rapid production and purification of exfoliative toxin A of Staphylococcus aureus

A novel method for rapid production and purification of exfoliative toxin A of Staphylococcus aureus

Guest Lecture*

Partial Purification and Some Characteristics of Proteinase(s) Induced by Staphylococcal Exfoliative Toxin

Contact Info

Product

Resources

About