Production and Antioxidant Activity of Intracellular Polysaccharide by Hypsizigus Marmoreus Sk-01

Abstract

The extraction parameters of intracellular polysaccharide (IPS) from Hypsizigus marmoreus SK-01 mycelia were optimized, and the antioxidant activities of IPS were investigated. The optimum conditions of IPS extraction were predicted to be: an ultrasonic treatment time of 618.07 s, an extraction temperature of 84.53 °C, a pH of 7.57, and an IPS yield of ca. 6.84%. The in vitro inhibition effects of IPS on hydroxyl, superoxide anion, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 52.63 ± 3.19%, 68.21 ± 5.09%, and 63.43 ± 5.27%, respectively, and the EC 50 values of IPS were 0.85 ± 0.05 g/L, 0.47 ± 0.03 g/L, and 0.62 ± 0.04 g/L, respectively. The activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) in mice blood were 2.52 ± 0.02 U/mL, 453.19 ± 38.43 U/mL, and 118.59 ± 9.64 U/mL, respectively. The results provide a reference for large-scale extraction of IPS by H. marmoreus SK-01 in industrial fermentation, suggesting that IPS can be used as a potential antioxidant that enhances adaptive immune responses.

Protective effects on liver, kidney and pancreas of enzymatic- and acidic-hydrolysis of polysaccharides by spent mushroom compost (Hypsizigus marmoreus)

“…Among the various nutrient substances obtained from mushrooms, polysaccharides have been demonstrated to possess a diversity of useful biological properties including anti-oxidant, anti-tumor, anti-bacterial, anti-inflammatory, immunomodulatory, anti-hyperglycemic and anti-hypercholesterolemic activities 14 . In light of these scientific findings, many investigators have devoted themselves to assessing the anti-diabetic effects of polysaccharides from either the fruiting body or mycelia, such as Phellinus baumii 15 , and Catathelasma ventricosum 16 .…”

Antioxidants from Natural Sources

“…Phenols [88] and polysaccharides are the major bioactive components present in H. marmoreus that show the antioxidant activity by scavenging reactive oxygen species and strengthening its reducing power [55]. Liu et al investigated the intracellular polysaccharides of H. marmoreus and revealed that these polysaccharides can be utilized as an antioxidant agent that improves adaptive immune reactions [89].…”

Antioxidant and anti-ageing activities of mycelia zinc polysaccharide fromPholiota namekoSW-03

“…content where A is the absorbance of the sample and A 0 is the absorbance of TEP. The content of malondialdehyde (MDA) was determined according to the method of Liu et al 24 with some modification. The reaction mixture, comprising 0.5 mL of sample, 1.5 mL of distilled water and 2 mL of TBA (6 g L −1 ), was heated in boiling water for 15 min.…”