Production and Biological Effects of Extracellular Vesicles from Adipose-Derived Stem Cells Were Markedly Increased by Low-Intensity Ultrasound Stimulation for Promoting Diabetic Wound Healing

Zheng, Yi; Xu, Peng; Pan, Chuqiao; Wang, Yikai; Liu, Zibo; Chen, Yahong; Chen, Chuhsin; Fu, Shibo; Xue, Ke; Zhou, Qimin; Liu, K

doi:10.1007/s12015-022-10487-w

Cited by 15 publications

(11 citation statements)

References 77 publications

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“…Numerous studies have identified MVs as an indicator of risk and having the power to promote or positively impact wound healing. In a study by Zheng et al, 28 the use of low-intensity ultrasound stimulation as a new method for promoting significant EV secretion in diabetic wounds promoted markedly increased wound healing in vitro and in vivo. In diabetic mice, Wei et al 29 found that EVs promote wound healing by reducing yes-associated protein 1 phosphorylation and activating the phosphoinositide 3-kinase, protein kinase B oncogene, mammalian target of rapamycin kinase pathway, therefore increasing vascular markers and fibroblast proliferation.…”

Section: Discussionmentioning

confidence: 99%

Wound Fluid Extracellular Microvesicles: A Potential Innovative Biomarker for Wound Healing

Mari,

Younes,

Sheehan

et al. 2024

Plastic and Reconstructive Surgery - Global Open

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Background: Extracellular vesicles, or microvesicles, are a large family of membrane-bound fluid-filled sacs that cells release into the extracellular environment. Extracellular microvesicles (EMVs) are essential for cell-to-cell communications that promote wound healing. We hypothesize a correlation between the concentration of EMVs in wound fluid and the percentage of wound healing in treated chronic, nonhealing, wounds. A prospective, multicenter, randomized, single-blind clinical trial was conducted to evaluate EMV concentration in relation to wound healing percentages. Methods: Wound fluid samples were obtained from 16 patients with stage IV trunk pressure ulcers. Patients were divided equally into two groups: (1) control group on negative pressure wound therapy (NPWT) alone and (2) study group with NPWT plus porcine extracellular matrix dressing. NPWT was replaced two times a week, and porcine extracellular matrix applied once weekly for all subjects. An NPWT canister device, called a wound vacuum-assisted closure, containing wound fluid was collected from each patient every 4 weeks. EMVs were isolated and the concentration measured by nanoparticle tracking analysis. Results: The study group’s total healing percentage was around 89% after 12 weeks compared with the control group’s percentage of about 52% (P ≤ 0.05). Using R programming software, simple linear regression was carried out to investigate the hypothesis. Data demonstrated significant positive correlation (R2 = 0.70; P = 0.05) between EMV concentrations and the healing percentage. Conclusions: There is a positive correlation between EMV concentration and wound healing percentages. Results propose that the EMVs in wound fluid could serve as a biomarker for healing.

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Section: Discussionmentioning

confidence: 99%

Wound Fluid Extracellular Microvesicles: A Potential Innovative Biomarker for Wound Healing

Mari,

Younes,

Sheehan

et al. 2024

Plastic and Reconstructive Surgery - Global Open

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“…[219,220] Second, their yields are so low that they cannot meet clinical needs. [221] Third, their in vivo half-life is unsatisfactory (e.g., unmodified EVs be rapidly cleared within 10 min). [222] Fourth, from a technical point of view, it is extremely challenging to maintain the activity and viability of cells, as well as the natural structure and function of EVs and cell membranes during isolation, purification, storage, and transportation.…”

Section: Discussionmentioning

confidence: 99%

Cell‐Based Biomaterials for Coronavirus Disease 2019 Prevention and Therapy

Wang

Xie

et al. 2023

Adv Healthcare Materials

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Coronavirus disease 2019 (COVID-19) continues to threaten human health, economic development, and national security. Although many vaccines and drugs have been explored to fight against the major pandemic, their efficacy and safety still need to be improved. Cell-based biomaterials, especially living cells, extracellular vesicles, and cell membranes, offer great potential in preventing and treating COVID-19 owing to their versatility and unique biological functions. In this review, the characteristics and functions of cell-based biomaterials and their biological applications in COVID-19 prevention and therapy are described. First the pathological features of COVID-19 are summarized, providing enlightenment on how to fight against COVID-19. Next, the classification, organization structure, characteristics, and functions of cell-based biomaterials are focused on. Finally, the progress of cell-based biomaterials in overcoming COVID-19 in different aspects, including the prevention of viral infection, inhibition of viral proliferation, anti-inflammation, tissue repair, and alleviation of lymphopenia are comprehensively described. At the end of this review, a look forward to the challenges of this aspect is presented.

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“…For instance, low-intensity ultrasound could enhance the generation of wound healing-related miRNAs in ADSC-exos, including let-7b-5p, miR-17-5p, miR-99b-3p, miR-106b-3p, miR-140-3p, miR-143-5p, miR-145-5p, miR-222-3p, miR-320a-3p, miR-423-5p, miR-493-3p, miR-589-5p, miR-94.Additionally, ultrasound-pretreated ADSC-exos could facilitate FB, KC, and EC proliferation and migration and increase diabetic wound healing in db/db mice in vivo through re-epithelialization, collagen production, and angiogenesis process. 63 Whereas, Wang et al found that hypoxia-preconditioned ADSC-exos could enhance the secretion of vascular growth factors and ECM via PI3K/AKT pathway activation, thereby promoting fibroblast proliferation and migration ability. Additionally, these EVs could also upregulate inflammatory factors and chemokines to improve the velocity and efficiency of diabetic wound healing 64 ( Figure 3 ).…”

Section: The Mechanism Of Stem Cell-evs In Diabetic Wound Healingmentioning

confidence: 99%

Stem Cell-Derived Extracellular Vesicles: Promising Therapeutic Opportunities for Diabetic Wound Healing

Zhang,

Bi,

Wang

et al. 2024

IJN

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Wound healing is a sophisticated and orderly process of cellular interactions in which the body restores tissue architecture and functionality following injury. Healing of chronic diabetic wounds is difficult due to impaired blood circulation, a reduced immune response, and disrupted cellular repair mechanisms, which are often associated with diabetes. Stem cell-derived extracellular vesicles (SC-EVs) hold the regenerative potential, encapsulating a diverse cargo of proteins, RNAs, and cytokines, presenting a safe, bioactivity, and less ethical issues than other treatments. SC-EVs orchestrate multiple regenerative processes by modulating cellular communication, increasing angiogenesis, and promoting the recruitment and differentiation of progenitor cells, thereby potentiating the reparative milieu for diabetic wound healing. Therefore, this review investigated the effects and mechanisms of EVs from various stem cells in diabetic wound healing, as well as their limitations and challenges. Continued exploration of SC-EVs has the potential to revolutionize diabetic wound care.

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Production and Biological Effects of Extracellular Vesicles from Adipose-Derived Stem Cells Were Markedly Increased by Low-Intensity Ultrasound Stimulation for Promoting Diabetic Wound Healing

Cited by 15 publications

References 77 publications

Wound Fluid Extracellular Microvesicles: A Potential Innovative Biomarker for Wound Healing

Wound Fluid Extracellular Microvesicles: A Potential Innovative Biomarker for Wound Healing

Cell‐Based Biomaterials for Coronavirus Disease 2019 Prevention and Therapy

Stem Cell-Derived Extracellular Vesicles: Promising Therapeutic Opportunities for Diabetic Wound Healing

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