Production and characterisation of exopolysaccharide from Streptomyces carpaticus isolated from marine sediments in Egypt and its effect on breast and colon cell lines

Abstract

Twenty streptomycete strains were isolated from marine sediment samples collected from Nabq area, Sharm El-Sheikh, Red Sea Coast, Egypt. Four of them produce exopolysaccharides (EPS) showing marked in vitro antitumor activities. Morphological and cultural characteristics of the most significant strain (No. 3) were shown. Moreover, the sequence of this strain showed similarity with Streptomyces carpaticus. The results reveal that EPS produced by Streptomyces carpaticus No. 3 had high cytotoxicity reaching 51.7% and 59.1% against human tumor cells of breast and colon lines respectively. A chemical analysis of EPS indicated that the composing monosaccharides were galactouronic acid, glucose, xylose, galactose, mannose, and fructose with relative ratio of 3:1:1:2:2:1 respectively, with an average molecular weight (Mw) 1.180 × 105 g/mol and of a number average molecular weight (Mn) 1.052 × 105 g/mol. Also the EPS contained uronic acid (0.5072%) and monosaccharide sulphates (21.753%).

Protective effect of some natural products against chemotherapy-induced toxicity in rats

“…Screening for natural substances with chemopreventive properties in order to reduce the negative effects of chemotherapeutic drugs is very urgent (Selim et al., 2018). So in this study three different natural product were used against chemotherapy-induced organ toxicity.…”

“…Thus, it is warranted to use different species for obtaining polysaccharides with better properties (Elsakhawy et al., 2017). Exopolysaccharides were first isolated from marine streptomycete in Egypt by Selim et al. (2018) and their antitumor effect have been tested only in colon and breast cancer cell lines.…”

Toward Rational Design of Novel Anti-Cancer Drugs Based on Targeting, Solubility, and Bioavailability Exemplified by 1,3,4-Thiadiazole Derivatives Synthesized Under Solvent-Free Conditions

“…This was done according to Selim et al [41], as described by Mosmann [42]. The cells were seeded at concentration of 10 × 10 3 cells per well in case of MCF-7, 20 × 10 3 cells/well in case of A-549 cell lines and 35–45 × 10 3 cells/well in case of BJ-1 using 96-well plates at 37 °C.…”

Protective Effect of Natural Products against Chemotherapy-Induced Cardiotoxicity: A Review

“…There is an urgent need to uncover the potential of natural products to ameliorate the negative effects of chemotherapeutic drugs. 87 Overall, most natural products have more than a single "target" and may affect multiple signaling pathways. Therefore, the development of natural products which can target at multiple sites may be useful in ameliorating the cardiotoxicity induced by chemotherapeutic agents.…”