Production and characteristics of protein hydrolysate from tra fish (Pangasius hypophthalmus)

Trinh, Khanh Son; Nguyễn, Thùy Linh

doi:10.18067/jbfs.v6i4.264

Cited by 1 publication

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References 27 publications

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“…Short-chain peptides appear to be more beneficial than both intact proteins and free amino acids (Clemente, 2000). Both pH and temperature strongly affected the enzymatic hydrolysis of fish protein (Trinh and Nguyen, 2019). Generally, FPH can be produced from fish meat and by-products such as skin, bone, etc.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant properties of hydrolyzate produced from the crude extract of <em>Hilsa kelee</em>

Meepegamage¹,

Egodauyana²,

Abeyrathne³

2022

AGRIEAST: J. Agri. Sci.

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Hilsa kelee also known as "Karattaya" is a seasonal fish mainly found in tropical coastal waters and used for fish feed production due to its low market value. Therefore, this study was designed to determine the functional properties of fish protein hydrolyzate (FPH) produced from the crude extract of Hilsa kelee. For extraction, 20 mg/mL concentrated water-soluble and salt-soluble crude extracts were mixed with protease, papain, α-chymotrypsin, and elastase in 1:100 (enzyme: substrate) ratios. Each sample was incubated at different time intervals (0, 3, 6, 9, 12, and 24 hours) under predetermined optimum pH and temperatures. SDS-PAGE, TBARS, and DPPH assays and ferrous chelation activity were measured in the selected best hydrolysates. According to SDS-PAGE analysis for water and salt extracted, the following hydrolysis time lags were selected, protease (3 h), α-chymotrypsin (6 h), Elastase (6 h) and papain (6 h for water and 3 h for salt extraction). In the TBARS assay, the FPHs from salt soluble proteins with papain showed the highest antioxidant properties (5.84±0.05 MDA mg/L) compared to other treatments (p<0.05). The DPPH assay showed the highest radical scavenging activity with salt soluble proteins hydrolyzed with α-chymotrypsin (p<0.05). Analysis of ferrous chelation activity showed that FPHs produced from water-soluble proteins with α-chymotrypsin (20.81±0.02%) had the highest chelation (p<0.05). This concludes, FPHs produced from Hilsa kelee using papain and α-chymotrypsin enzymes contain antioxidant properties, and iron-binding activity.

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Section: Introductionmentioning

confidence: 99%

Antioxidant properties of hydrolyzate produced from the crude extract of <em>Hilsa kelee</em>

Meepegamage¹,

Egodauyana²,

Abeyrathne³

2022

AGRIEAST: J. Agri. Sci.

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Production and characteristics of protein hydrolysate from tra fish (Pangasius hypophthalmus)

Abstract: Produção e características do hidrolisado de proteínas de peixepanga (Pangasius hypophthalmus

Cited by 1 publication

References 27 publications

Antioxidant properties of hydrolyzate produced from the crude extract of <em>Hilsa kelee</em>

Antioxidant properties of hydrolyzate produced from the crude extract of <em>Hilsa kelee</em>

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