Production and characterization of a monoclonal antibody against the β-subunit of bullfrog lutropin

Park, Min Kyun; Tanaka, Shigeyasu; Hayashi, Hiroaki; Hanaoka, Yoichi; Wakabayashi, Keiji; Kurosumi, Kazumasa

doi:10.1016/0016-6480(87)90063-3

Cited by 19 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sections were counterstained with hematoxylin for 10 s, and coverslipped. The specificities of the MCAs used were described by Park et al (1987) and Tanaka et al (1990). In all cases two controls of immunocytochemical method were done: (1) Primary antibody was omitted and replaced by Phosphate Buffered Saline (PBS); (2) Primary antibody was omitted and replaced by antiserum preabsorbed with homogenate of Bufo arenarum pituitaries (1 mg with 1 ml antiserum).…”

Section: Methodsmentioning

confidence: 99%

Immunocytochemical study of pituitary FSHß and LHß cells during the ontogeny of the toad Bufo arenarum

1998

View full text Add to dashboard Cite

The ontogeny of gonadotrophs in the pituitary of Bufo arenarum was studied by immunocytochemical techniques using monoclonal antibodies against bullfrog lutropin ß-subunit and bullfrog follitropin ß-subunit. The first appearance of immunoreactive gonadotropic cells was at midprometamorphosis in the caudal zone of the pars distalis. During metamorphosis, in juveniles and especially in adult animals, follicle stimulating hormone (FSH) and luteinizing hormone (LH) producing cells were distributed throughtout the gland. The number of these cells was greatly increased in older stages. In all the stages studied colocalization of FSH and LH was observed. At least three types of gonadotrophs were identified: the first cell type containing only FSH; the second only LH; and the third, FSH and LH in the same cell.

show abstract

Section: Methodsmentioning

confidence: 99%

Immunocytochemical study of pituitary FSHß and LHß cells during the ontogeny of the toad Bufo arenarum

1998

View full text Add to dashboard Cite

show abstract

“…To identify the pituitary cells, we employed mouse monoclonal antibody against the bullfrog luteinizing hormone β-subunit (LHβ) (Park et al 1987), mouse monoclonal anti-rat PRL (Chemicon, Temecula, CA, USA), guinea pig anti-human growth hormone (GH) (Kato et al 2004), guinea pig anti-bullfrog adrenocorticotropin (ACTH) 1-39 ) and goat anti-human thyroid-stimulating hormone (TSH) β-subunit (Biogenesis, New Fields, UK).…”

Section: Antibodiesmentioning

confidence: 99%

Expression of a mammalian aquaporin 3 homolog in the anterior pituitary gonadotrophs of the tree frog, Hyla japonica

et al. 2011

Self Cite

View full text Add to dashboard Cite

Aquaporins (AQPs) are a family of water channel proteins that play a major role in maintaining water homeostasis in various organisms. Several AQPs have been identified in the tree frog, Hyla japonica. Of these, AQP-h3BL, which is expressed in the basolateral membrane of the epithelial cells, is a homolog of mammalian AQP3. Using immunohistochemistry and in situ RT-PCR, we have demonstrated that AQP-h3BL is expressed in the anterior pituitary gonadotrophs of the tree frog but not in the other hormone-producing cells of the anterior pituitary. In gonadotrophs labeled for luteinizing hormone subunit-β (LHβ), AQP-h3BL protein was found to reside in the plasma membrane, the nuclear membrane and the cytoplasm. Double-labeling of AQP-h3BL mRNA and LHβ protein revealed that AQP-h3BL mRNA is expressed in the gonadotrophs. Following stimulation by gonadotropin-releasing hormone (GnRH), the label for AQP-h3BL localized in the plasma membrane became more intense, concomitant with the transport of LHβ-positive materials to the plasma membrane. These developments coincided with a decrease in the labeling density in the cytoplasm and near the nuclear membrane, suggesting that the latter localizations may function as "storage area" for AQP-h3BL. Immunoelectron microscopy also confirmed these localizations of AQP-h3BL protein. Based on these results, we suggest that AQP-h3BL protein in the frog gonadotrophs is involved in the formation of secretory granules, the swelling and increase in the volume of the granules and exocytosis.

show abstract

Distribution of Endothelin 3-Like Immunoreactivity in Gonadotrophs of the Bullfrog (Rana catesbeiana) Pituitary

Suzuki

Yamamoto

Kikuyama

et al. 1997

General and Comparative Endocrinology

View full text Add to dashboard Cite

Production and characterization of a monoclonal antibody against the β-subunit of bullfrog lutropin

Cited by 19 publications

References 18 publications

Immunocytochemical study of pituitary FSHß and LHß cells during the ontogeny of the toad Bufo arenarum

Immunocytochemical study of pituitary FSHß and LHß cells during the ontogeny of the toad Bufo arenarum

Expression of a mammalian aquaporin 3 homolog in the anterior pituitary gonadotrophs of the tree frog, Hyla japonica

Distribution of Endothelin 3-Like Immunoreactivity in Gonadotrophs of the Bullfrog (Rana catesbeiana) Pituitary

Contact Info

Product

Resources

About