Production and characterization of a high molecular weight levan and fructooligosaccharides from a rhizospheric isolate of Bacillus aryabhattai

Nasir, Anam; Sattar, Fazal; Ashfaq, Iram; Lindemann, Stephen R.; Chen, Ming Hsu; Ende, Wim Van den; Öner, Ebru Toksoy; Kırtel, Onur; Khaliq, Shazia; Ghauri, Muhammad Afzal; Anwar, Munir A.

doi:10.1016/j.lwt.2020.109093

Cited by 31 publications

(15 citation statements)

References 45 publications

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“…The linkage analysis method of Nasir et al [ 11 ] was used, with minor modifications. In total, 10 mg of the LPB8-1 sample was dissolved in anhydrous dimethyl sulfoxide and methylated with 0.5 mL methyl iodine.…”

Section: Methodsmentioning

confidence: 99%

Extraction, Structural Analysis, and Biofunctional Properties of Exopolysaccharide from Lactiplantibacillus pentosus B8 Isolated from Sichuan Pickle

Jiang

et al. 2022

Foods

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Two novel exopolysaccharides, named LPB8-0 and LPB8-1, were isolated and purified from Lactiplantibacillus pentosus B8. Moreover, their structure and bioactivities were evaluated through chemical and spectral means. The study results demonstrated that LPB8-0 was primarily composed of mannose and glucose and had an average molecular weight of 1.12 × 104 Da, while LPB8-1 was composed of mannose, glucose, and galactose and had an average molecular weight of 1.78 × 105 Da. Their carbohydrate contents were 96.2% ± 1.0% and 99.1% ± 0.5%, respectively. The backbone of LPB8-1 was composed of (1→2)-linked α-D-Manp and (1→6)-linked α-D-Manp. LPB8-0 and LPB8-1 had semicrystalline structures with good thermal stability (308.3 and 311.7 °C, respectively). SEM results displayed that both LPB8-0 and LPB8-1 had irregular thin-slice shapes and spherical body structures. Additionally, an emulsifying ability assay confirmed that LPB8-0 and LPB8-1 had good emulsifying activity against several edible oils, and this activity was retained under acidic, neutral, and high temperature conditions. Furthermore, an antioxidant assay confirmed that LPB8-1 had stronger scavenging activity than LPB8-0. Overall, these results provide a theoretical basis for the potential application of these two novel exopolysaccharides as natural antioxidants and emulsifiers in the food and pharmaceutical industries.

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Section: Methodsmentioning

confidence: 99%

Extraction, Structural Analysis, and Biofunctional Properties of Exopolysaccharide from Lactiplantibacillus pentosus B8 Isolated from Sichuan Pickle

Jiang

et al. 2022

Foods

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“…13 C NMR spectroscopy was performed to identify the glycosidic linkages in the Apilactobacillus waqarii EPS by the method previously used for microbial EPS [ 80 ].…”

Section: Methodsmentioning

confidence: 99%

High-Molecular-Weight Dextran-Type Exopolysaccharide Produced by the Novel Apilactobacillus waqarii Improves Metabolic Syndrome: In Vitro and In Vivo Analyses

Ahmad

Boyajian

Abosalha

et al. 2022

IJMS

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Metabolic syndrome is a leading medical concern that affects one billion people worldwide. Metabolic syndrome is defined by a clustering of risk factors that predispose an individual to cardiovascular disease, diabetes and stroke. In recent years, the apparent role of the gut microbiota in metabolic syndrome has drawn attention to microbiome-engineered therapeutics. Specifically, lactic acid bacteria (LAB) harbors beneficial metabolic characteristics, including the production of exopolysaccharides and other microbial byproducts. We recently isolated a novel fructophilic lactic acid bacterium (FLAB), Apilactobacillus waqarii strain HBW1, from honeybee gut and found it produces a dextran-type exopolysaccharide (EPS). The objective of this study was to explore the therapeutic potential of the new dextran in relation to metabolic syndrome. Findings revealed the dextran’s ability to improve the viability of damaged HT-29 intestinal epithelial cells and exhibit antioxidant properties. In vivo analyses demonstrated reductions in body weight gain and serum cholesterol levels in mice supplemented with the dextran, compared to control (5% and 17.2%, respectively). Additionally, blood glucose levels decreased by 16.26% following dextran supplementation, while increasing by 15.2% in non-treated mice. Overall, this study displays biotherapeutic potential of a novel EPS to improve metabolic syndrome and its individual components, warranting further investigation.

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“…Levan’s molecular weight varies with the type of bacteria used for its synthesis and the cultivation parameters. Levan produced by the Bacillus aryabhattai GYC2-3 strain had a high average molecular weight (5.317 × 10 7 Da) [ 166 ], while levan from Bacillus licheniformis 8-37-0-1 had a low molecular weight (2.826 × 10 4 Da) [ 160 ]. The sucrose concentration was found to be the critical component in modulating the molecular weight of synthesized levan.…”

Section: Types and Properties Of Bacterial Exopolysaccharidesmentioning

confidence: 99%

Biomedical Applications of Bacterial Exopolysaccharides: A Review

et al. 2021

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Bacterial exopolysaccharides (EPSs) are an essential group of compounds secreted by bacteria. These versatile EPSs are utilized individually or in combination with different materials for a broad range of biomedical field functions. The various applications can be explained by the vast number of derivatives with useful properties that can be controlled. This review offers insight on the current research trend of nine commonly used EPSs, their biosynthesis pathways, their characteristics, and the biomedical applications of these relevant bioproducts.

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Production and characterization of a high molecular weight levan and fructooligosaccharides from a rhizospheric isolate of Bacillus aryabhattai

Cited by 31 publications

References 45 publications

Extraction, Structural Analysis, and Biofunctional Properties of Exopolysaccharide from Lactiplantibacillus pentosus B8 Isolated from Sichuan Pickle

Extraction, Structural Analysis, and Biofunctional Properties of Exopolysaccharide from Lactiplantibacillus pentosus B8 Isolated from Sichuan Pickle

High-Molecular-Weight Dextran-Type Exopolysaccharide Produced by the Novel Apilactobacillus waqarii Improves Metabolic Syndrome: In Vitro and In Vivo Analyses

Biomedical Applications of Bacterial Exopolysaccharides: A Review

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