Production and characterization of a bioflocculant by Proteus mirabilis TJ-1

Xia, Siqing; Zhang, Zhiqiang; Wang, Xuejiang; Aming, Yang; Chen, Ling; Zhao, Jianfu; Léonard, Didier; Jaffrézic‐Renault, Nicole

doi:10.1016/j.biortech.2007.11.031

Cited by 189 publications

(150 citation statements)

References 31 publications

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“…The growth curve of P. mirabilis TJ1 showed that the production of its bioflocculant was almost parallel with cell growth. The bioflocculant of P. mirabilis TJ1 reached its maximum flocculation rate (93.13 %) at stationary phase (Xia et al, 2008). In the same way, production of the bioflocculant by Enterobacter aerogenes was in parallel with cell growth and reached its maximum flocculation rate in early stationary phase (Lu et al, 2005).…”

Section: Pseudomonas Aeruginosa Fm207533mentioning

confidence: 71%

Characterization of bioflocculants produced by bacteria isolated from crude petroleum oil

Zaki

Farag

Elreesh

et al. 2011

Int. J. Environ. Sci. Technol.

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ABSTRACT:The aim of this study was to look for high efficient bioflocculant-producing microorganisms. Among 36 bacterial colonies isolated from a crude petroleum oil sample, three of them as Bacillus subtilis and Pseudomonas spp. exhibited flocculation activity exceeding 90 % after 3 days of cultivation. They were identified by 16 S rDNA sequence analysis as Bacillus subtilis and Pseudomonas sp. Spectroscopic analysis of the polymers by nuclear magnetic resonance and fourier-transform infrared revealed that the polymers were glycoproteins. These polymers were soluble in water and insoluble in any organic solvents tested. The effects of bioflocculant dosage, temperature and pH on the flocculation activity were evaluated. The maximum bioflocculation activities were observed at an optimum bioflocculant dosage of 3.5 mg/L (strains Bacillus subtilis and Pseudomonas) and 5.0 mg/L (strain CPO14), respectively. In addition, these biopolymers were able to flocculate kaolin suspension (5 g/L) over a wide range of pH (pH 3-9) and temperature (5-50 °C) tested in the presence of CaCl 2 . The highest flocculation activities of strains CPO8, CPO13 and CPO14 were 96.03 %, 92.17 % and 97.59 %, respectively in the early stationary phase (at 24 h), while the cell production reached its maximum in the stationary phase (at 72 h). Their efficient flocculation capabilities suggest potential applications in industries.

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Section: Pseudomonas Aeruginosa Fm207533mentioning

confidence: 71%

Characterization of bioflocculants produced by bacteria isolated from crude petroleum oil

Zaki

Farag

Elreesh

et al. 2011

Int. J. Environ. Sci. Technol.

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“…2,[20][21][22] The effects of the key factors, including culture time, carbon source, nitrogen source, C/N ratio, metal ions and ionic strength, initial pH of the production medium, culture temperature, inoculum size and shaking speed on the flocculating activity of CBF, were investigated to identify the optimal culture conditions for the CBF production.…”

Section: Cbf Productionmentioning

confidence: 99%

“…Many reported strains could effectively use organic nitrogen sources or organic nitrogen combined with inorganic nitrogen sources to produce bioflocculants. 22,23,26 For example, peptone (organic nitrogen source) and sodium nitrate (inorganic nitrogen source) provide the best nitrogen source to Aspergillus parasiticus for bioflocculant production, whereas with (NH 4 ) 2 SO 4 , no flocculant is produced. 26 Compared with inorganic sources, urea and yeast extract are more favorable for bioflocculant production by S. ficaria S-14, but a complex nitrogen source that consists of beef extract and urea is better than solely inorganic or organic nitrogen source.…”

Section: Cbf Productionmentioning

confidence: 99%

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Production of a novel bioflocculant and its flocculation performance in aluminum removal

Zuo

2016

Bioengineered

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A novel bioflocculant CBF with high flocculating activity, produced by mixed culture of Rhizobium radiobacter F2 and Bacillus sphaericus F6 from soil, was investigated with regard to its production and flocculation performance in Al(III) removal. The most preferred carbon source, nitrogen source and C/N ratio (w/w) for strains F2 and F6 to produce CBF were glucose, urea and 20, respectively. The optimal inoculum size for CBF production was 10 % (v/v). The optimal initial pH, culture temperature and shaking speed were 7-8, 30C and 140 r/min for 24 h, respectively, under which the flocculating activity of the bioflocculant reached 98.52 %. According to literature review, flocculant dosage, coagulant aid dosage, pH, hydraulic condition of coagulation and sedimentation time are considered as influencing parameters for CBF flocculation performance in Al(III) removal by L16 (4 5 ) orthogonal design. The optimal conditions for Al(III) removal obtained through analysis and verification experiments were as follows: CBF, 28 mg/L; coagulant aid, 1.5 mL/L; initial pH, 8.0; and hydraulic conditions of coagulation: stir speed, 160 r/min; stir time, 40 s; and sedimentation time, 30 min. Under the optimal conditions, the removal efficiency of Al(III) was 92.95 %. Overall, these findings indicate that bioflocculant CBF offers an effective alternative method of decreasing Al(III) during drinking water treatment.

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“…3 shows that pH 6 and 7 were more favourable for MBF-UFH production with the highest flocculating activity observed at pH 6. It has been well established in several studies that the initial pH of the production medium greatly influences bioflocculant production as it determines the electric charge of the cells and redox potential, which affects nutrient absorption as well as influencing the rate of enzymatic reaction [35,48]. Similarly, Noghabi et al [36] found that the production of extracellular biopolymer by Pseudomonas fluorescens BM07 was favourable between pH 6-8.…”

Section: Effect Of Nitrogen Source On Mbf-ufh Productionmentioning

confidence: 99%