Production and Characterization of Antimicrobial Active Compounds From the Cyanobacterium Nostoc Commune Vauch

Abstract

In this investigation, antimicrobial activity of Nostoc commune Vauch (isolated from agricultural wastewater canal, Beni Suef Governorate, Egypt) organic extracts were examined against nine selected microbial isolates. Four of them were Gram positive bacterial isolates (Bacillus subtilis, Mycobacterium phlei, Sarcina maxima and Staphylococcus aureus), four Gram negative bacteria (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa and Salmonella arizonae) and one unicellular fungus (Candida albicans) were evaluated for their resistance against these extracts. Methanol was the best organic solvent for extraction of active material, rather than the other organic solvents. This material was produced, maximally, after 10 days of incubation in aerated shaken culture at 30ºC and pH 8.0 when N. commune was grown in Medium 18 growth medium. The antagonistic material was purified using thin layer chromatography then identified using chromatographic and spectroscopic techniques including UV, FT-IR, mass spectrophotometer and proton-NMR. Four unknown compounds were extracted that had long chain alcohol, sterol, long chain fatty acid and triterpen. These compounds were tested for their antimicrobial activity against B. subtilis. Only the long chain fatty acid (compound C) had an inhibitory effect on the growth of B. subtilis while the other compounds were not active.

Effect of Cyanobacteria (Nostoc species) Extracts on Osteogenesis Activities

“…It has also been reported to have interesting biological activities such as anti-oxidant, anti-microbial, anti-bacterial, anti-fungal, cholinesterase inhibitory, a cytotoxic effect on cancer cells, and hypercholesterolemic effects [8]. It also has a wide range of bioactive compounds, which include carotenoids, phycocyanin, total phenols, tannin, alkaloids, flavonoids, and terpenoids similar to previous literature on phytochemical compounds [9][10][11][12]. However, studies on cyanobacteria especially in Nostoc species for the osteogenensis effect have been very limited.…”

Cyanobacteria: A Promising Source of Antifungal Metabolites

“…Some bioproducts of cyanobacteria are dependent on the limitations of one or more nutrients [ 307 , 308 , 309 , 310 , 311 ]. For instance, the Scyto accumulation in S. ocellatum ATCC 55232 noticeably varies depending on the growing medium.…”