2009
DOI: 10.1270/jsbbs.59.109
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Production and characterization of Brassica napus-Raphanus sativus monosomic addition lines mediated by the synthetic amphidiploid "Raphanobrassica"

Abstract: In order to breed Brassica napus-Raphanus sativus monosomic addition lines (MALs), hybridizations between two synthetic amphidiploid (RRAA and RRCC) and B. napus (AACC) were performed. Two allooctoploids (RRAAAACC and AACCRRCC) were produced from each F 1 hybrid by chromosome doubling. From successive backcrosses to B. napus, MAL plants were first obtained in BC 2 generation and the R. sativus-derived chromosome was identified by genomic in situ hybridization (GISH). The nine chromosomes of R-genome in the MAL… Show more

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Cited by 13 publications
(15 citation statements)
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“…The c-type MAL with the genetic background of rape and the clubroot resistance of radish should be useful as a bridge plant to introduce the resistance gene of radish into rape. However, it is difficult to introduce the resistance gene(s) through recombination between the c-chromosome of radish and the corresponding chromosome of rape because of the rare syndesis between these chromosomes (Akaba et al 2009). To promote the introgression of the resistance gene(s) on the c-chromosome, translocation between the c-chromosome of radish and the corresponding one of rape could be induced by γ-ray irradiation (Kaneko et al 1992).…”
Section: Resultsmentioning
confidence: 99%
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“…The c-type MAL with the genetic background of rape and the clubroot resistance of radish should be useful as a bridge plant to introduce the resistance gene of radish into rape. However, it is difficult to introduce the resistance gene(s) through recombination between the c-chromosome of radish and the corresponding chromosome of rape because of the rare syndesis between these chromosomes (Akaba et al 2009). To promote the introgression of the resistance gene(s) on the c-chromosome, translocation between the c-chromosome of radish and the corresponding one of rape could be induced by γ-ray irradiation (Kaneko et al 1992).…”
Section: Resultsmentioning
confidence: 99%
“…To promote the introgression of the resistance gene(s) on the c-chromosome, translocation between the c-chromosome of radish and the corresponding one of rape could be induced by γ-ray irradiation (Kaneko et al 1992). In addition, many c-chromosome specific RAPD markers were developed in the previous study (Akaba et al 2009) that could be helpful for the development of DNA markers linked to the resistance loci.…”
Section: Resultsmentioning
confidence: 99%
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