Production and characterization of cellulolytic enzymes from the thermophilic fungus Thermoascus aurantiacus under solid state cultivation of agricultural wastes

Kalogeris, Emmanuel; Christakopoulos, Paul; Katapodis, Petros; Alexiou, A.; Vlachou, S; Kekos, Dimitris; Macris, Basil J.

doi:10.1016/s0032-9592(02)00242-x

Cited by 141 publications

(64 citation statements)

References 24 publications

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“…This stability in acid pH values shows the potential application of such enzyme in industrial bioprocesses such as the production of bioethanol (PEIXOTO-NOGUEIRA et al, 2009). The xylanase enzyme also showed high stability up to 55 °C ( Figure 3D) and kept 86% of the maximum activity at 50 °C, indicating a higher stability when compared to other studies (KALOGERIS et al, 2003;KANG et al, 2004). The effect of sugars which are released after the hydrolysis of lignocellulosic biomass was evaluated on xylanase activity, as well as the inhibitory effect of the ethanol as the final product of the alcoholic fermentation.…”

Section: Resultsmentioning

confidence: 66%

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Gomes¹,

Santos²,

Franciscon³

et al. 2016

Biosci. J.

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This study reports the optimization of xylanase production under solid state fermentation (SSF) by a thermotolerant Aspergillus fumigatus strain (SCB4) isolated from sugarcane bagasse piles of Brazilian Cerrado. Different combinations of low-cost agricultural byproducts in SSF were evaluated: sugarcane bagasse and wheat bran (1:1), sugarcane bagasse and corn straw (1:1) and only sugarcane bagasse. The enzyme biosynthesis by SSF was carried out at different temperatures (40, 45, 50 and 55 o C). The maximum levels of xylanase activity were obtained after 24 h at 45 °C using a culture medium containing sugarcane bagasse and wheat bran (1:1). Under optimal conditions, the fungal culture produced 574 U g -1 of xylanase (units/g of dry substrate). The crude enzyme showed optimal activity at 60 °C and pH 4.5. It exhibited thermostability up to 55 °C, wide range of pH stability and tolerance to ethanol, xylose and glucose. The physicochemical properties shown by this enzyme are appropriate for its application in hydrolysis of lignocellulosic residues for ethanol production and other bioproducts.

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Section: Resultsmentioning

confidence: 66%

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Gomes¹,

Santos²,

Franciscon³

et al. 2016

Biosci. J.

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“…In previous reports, inorganic nitrogen sources were optimal for the acquisition of maximum lignocellulolytic enzyme activity (Kalogeris et al 2003a). Urea, the most important inorganic nitrogen source, was used in this study.…”

Section: Effect Of Factor Levelmentioning

confidence: 97%

“…It is well known that fungi favor a moist environment as they grow. The optimal moisture content during the SSF depends on the natural requirements of the microbe, the type of the end products, and the nature of the substrate (Kalogeris et al 2003a). Hence, the influence of moisture content was investigated for VR degradation by consortium-29.…”

Section: Effect Of Factor Levelmentioning

confidence: 99%

Degradation of Lignocellulosic Components in Un-pretreated Vinegar Residue Using an Artificially Constructed Fungal Consortium

Cui¹,

Dong²,

Tong³

et al. 2015

BioResources

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The objective of this work was to degrade lignocellulosic components in un-pretreated vinegar residue (VR) using a fungal consortium. consisting of P. chrysosporium, T. koningii, A. niger, ), respectively. The cellulose, hemicellulose, and lignin contents decreased by 17.11%, 68.61%, and 14.44%, respectively, compared with that of the raw VR. The optimal fermentation conditions of consortium-29 were as follows: incubation temperature 25 °C, initial pH 6, initial moisture content 70%, inoculum size 1  10 6 spores/mL, incubation time 5 days, urea/VR 1%, and MnSO4H2O/VR 0.03%. This study suggests that consortium-29 is an efficient fungal consortium for un-pretreated VR degradation and has a potential application in lignocellulosic waste utilization with a low cost of operation.

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“…Previous studies reported the fungus T. aurantiacus as one of the largest producers of cellulases and hemicellulases, mainly when grown in wheat derivatives (PARRY et al, 2001;KALOGERIS et al, 2003;DA-SILVA et al, 2005), preventing the application of the enzymatic extract in biopulping processes. However, the T. aurantiacus strain evaluated in the present study showed low potential for the production of cellulases (Table 2).…”

Section: Catalytic Potential Of the Enzymatic Extractmentioning

confidence: 99%

“…Previous studies have described different strains of the filamentous fungi Thermoascus aurantiacus as excellent producers of xylanases and cellulases, particularly when grown in agroindustrial residues (KALOGERIS et al, 2003;DA-SILVA et al, 2005), precluding the application of these enzymatic extracts in cellulose pulp bleaching processes due to the high concentration of cellulolytic enzymes. Recently, our research group isolated a new strain of T. aurantiacus with considerable potential for the production of xylanase and reduced cellulolytic activity.…”

Section: Introductionmentioning

confidence: 99%

Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries

Costa¹,

Scalabrini²,

Silvestre³

et al. 2016

Biosci. J.

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ABSTRACT:Xylanases are useful in several industrial segments, including pulp and paper bleaching, animal feed, and bread-making processes. However, the industrial use of these enzymes is closely related to its production cost and its catalytic properties. The process of solid state fermentation enables the use of agro-industrial residues as substrates for microbial cultivation and enzymes production, reducing costs. In the present study, different cultivation parameters were evaluated for the xylanase production by the thermophilic fungus Thermoascus aurantiacus, by solid state fermentation, using agro-industrial residues as substrates. High production of xylanase (1701.9 U g -1 of dry substrate) was obtained using wheat bran containing 65% of initial moisture, at 120 h of cultivation, and 45°C. The xylanase showed optimal activity at pH 5.0 and 75°C; its stability was maintained at pH 3.0-11.0. The enzyme retained its catalytic potential after 1 h, at 75°C. The enzymatic extract produced under optimized conditions showed reduced activities of endoglucanase and FPase. Our results, including the xylanase production by T. aurantiacus in low-cost cultivation medium, high structural stability of the enzyme, and reduced cellulolytic activity, encourage the application of this enzymatic extract in pulp and paper bleaching processes.

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Production and characterization of cellulolytic enzymes from the thermophilic fungus Thermoascus aurantiacus under solid state cultivation of agricultural wastes

Cited by 141 publications

References 24 publications

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Degradation of Lignocellulosic Components in Un-pretreated Vinegar Residue Using an Artificially Constructed Fungal Consortium

Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries

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