Production and characterization of ECM powder: implications for tissue engineering applications

Gilbert, Thomas W.; Stolz, Donna B.; Biancaniello, Frank S.; Simmons-Byrd, Abby; Badylak, Stephen F.

doi:10.1016/j.biomaterials.2004.04.042

Cited by 130 publications

(87 citation statements)

References 18 publications

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“…The frozen sheets were comminuted into a particulate form using a Waring commercial blender and Wiley Mill with a #60 mesh screen. 25 …”

Section: Preparation Of Ecm Powdersmentioning

confidence: 99%

“…UBM-ECM was prepared as previously described. [24][25][26] In brief, connective and adipose tissue were removed from the serosal surface of the bladder, and the tunica serosa, tunica muscularis externa, tunica submucosa, and most of the tunica muscularis mucosa were mechanically removed. The luminal urothelial cells of the tunica mucosa were dissociated by soaking in a 1.0-N saline solution, leaving a biomaterial composed of only the basement membrane and the subjacent tunica propria of the tunica mucosa and the resident cell population of those 2 layers.…”

Section: Preparation Of Ecm Powdersmentioning

confidence: 99%

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Antibacterial Activity within Degradation Products of Biological Scaffolds Composed of Extracellular Matrix

Brennan

Reing²,

Chew³

et al. 2006

Tissue Engineering

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210

133

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Biological scaffolds composed of extracellular matrix (ECM) have been shown to be resistant to deliberate bacterial contamination in preclinical in vivo studies. The present study evaluated the degradation products resulting from the acid digestion of ECM scaffolds for antibacterial effects against clinical strains of Staphylococcus aureus and Escherichia coli. The ECM scaffolds were derived from porcine urinary bladder (UBM-ECM) and liver (L-ECM). These biological scaffolds were digested with acid at high temperatures, fractionated using ammonium sulfate precipitation, and tested for antibacterial activity in a standardized in vitro assay. Degradation products from both UBM-ECM and L-ECM demonstrated antibacterial activity against both S. aureus and E. coli. Specific ammonium sulfate fractions that showed antimicrobial activity varied for the 2 different ECM scaffold types. The results of this study suggest that several different lowmolecular-weight peptides with antibacterial activity exist within ECM and that these peptides may help explain the resistance to bacterial infection provided by such biological scaffolds.

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“…The frozen sheets were comminuted into a particulate form using a Waring commercial blender and Wiley Mill with a #60 mesh screen. 25 …”

Section: Preparation Of Ecm Powdersmentioning

confidence: 99%

Section: Preparation Of Ecm Powdersmentioning

confidence: 99%

Antibacterial Activity within Degradation Products of Biological Scaffolds Composed of Extracellular Matrix

Brennan

Reing²,

Chew³

et al. 2006

Tissue Engineering

Self Cite

210

133

View full text Add to dashboard Cite

show abstract

“…Peracetic acid residue was removed by washing twice with sterile PBS (pH 7.4) and twice with sterile water for 15 min each time. UBM sheets were subsequently lyophilized and comminuted to form a powder [23]. 1 g lyophilized UBM powder and 100 mg pepsin were added to 100 ml of 0.01 M HCl under mechanical stirring for 48 h at room temperature.…”

Section: Urinary Bladder Matrix Preparationmentioning

confidence: 99%

Hybrid nanofibrous scaffolds from electrospinning of a synthetic biodegradable elastomer and urinary bladder matrix

Stankus

Freytes

Badylak

et al. 2008

Journal of Biomaterials Science, Polymer Edition

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101

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Synthetic materials can be electrospun into submicron or nanofibrous scaffolds to mimic extracellular matrix (ECM) scale and architecture with reproducible composition and adaptable mechanical properties. However, these materials lack the bioactivity present in natural ECM. ECM-derived scaffolds contain bioactive molecules that exert in vivo mimicking effects as applied for soft tissue engineering, yet do not possess the same flexibility in mechanical property control as some synthetics. The objective of the present study was to combine the controllable properties of a synthetic, biodegradable elastomer with the inherent bioactivity of an ECM derived scaffold. A hybrid electrospun scaffold composed of a biodegradable poly(ester-urethane)urea (PEUU) and a porcine ECM scaffold (urinary bladder matrix, UBM) was fabricated and characterized for its bioactive and physical properties both in vitro and in vivo. Increasing amounts of PEUU led to linear increases in both tensile strength and breaking strain while UBM incorporation led to increased in vitro smooth muscle cell adhesion and proliferation and in vitro mass loss. Subcutaneous implantation of the hybrid scaffolds resulted in increased scaffold degradation and a large cellular infiltrate when compared with electrospun PEUU alone. Electrospun UBM/PEUU combined the attractive bioactivity and mechanical features of its individual components to result in scaffolds with considerable potential for soft tissue engineering applications.

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“…Several combination ECM materials isolated from mammalian sources are commercially available [52,77] , while others are in various stages of development and characterization [19,[78][79][80] .…”

Section: Polymersmentioning

confidence: 99%

Biomaterials for spinal cord repair

Haggerty

Oudega

2013

Neurosci. Bull.

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Spinal cord injury (SCi) results in permanent loss of function leading to often devastating personal, economic and social problems. A contributing factor to the permanence of SCi is that damaged axons do not regenerate, which prevents the re-establishment of axonal circuits involved in function. Many groups are working to develop treatments that address the lack of axon regeneration after SCi. The emergence of biomaterials for regeneration and increased collaboration between engineers, basic and translational scientists, and clinicians hold promise for the development of effective therapies for SCi. A plethora of biomaterials is available and has been tested in various models of SCi. Considering the clinical relevance of contusion injuries, we primarily focus on polymers that meet the specific criteria for addressing this type of injury. Biomaterials may provide structural support and/or serve as a delivery vehicle for factors to arrest growth inhibition and promote axonal growth. Designing materials to address the specific needs of the damaged central nervous system is crucial and possible with current technology. Here, we review the most prominent materials, their optimal characteristics, and their potential roles in repairing and regenerating damaged axons following SCi.Keywords: spinal cord injury; axon regeneration; biodegradable materials; extracellular matrix proteins; functional recovery; growth factor; guidance; injury and repair; spinal motor neuron IntroductionSpinal cord injury (SCi) causes loss of neurons and axons resulting in motor and sensory function impairments. There are thousands of new cases of SCi in the world annually [1,2] occurring often in young adults. The lack of endogenous repair and the significant costs to the individual, family and society [1] are important motivations behind the continuing efforts to develop effective therapies. Promoting axonal regeneration is considered a potential repair strategy because it could lead to recovery of axonal circuits involved in motor and/or sensory function [3] .The central nervous system (CNS) neurons are intrinsically capable of some regeneration of damaged axons [4] but their attempts after SCi are frustrated by structural and chemical obstructions in the damaged nervous tissue [5] . Spinal cord repair approaches typically lead to small functional gains. one feature that most of these approaches have in common is a poor axonal regeneration response, suggesting that promoting axonal regeneration, including synapse formation, is essential to achieve substantial functional repair after SCi. if so, it is rational to anticipate that effective spinal cord therapies will need to include interventions addressing the axon growth-inhibition that leads to the poor axonal growth responses. As introduced above, axonal regeneration is considered an important repair mechanism for the injured spinal cord. Therefore, this review focuses on materials that have shown most promise to elicit an axonal regeneration response to foster functional restoration after ...

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Production and characterization of ECM powder: implications for tissue engineering applications

Cited by 130 publications

References 18 publications

Antibacterial Activity within Degradation Products of Biological Scaffolds Composed of Extracellular Matrix

Antibacterial Activity within Degradation Products of Biological Scaffolds Composed of Extracellular Matrix

Hybrid nanofibrous scaffolds from electrospinning of a synthetic biodegradable elastomer and urinary bladder matrix

Biomaterials for spinal cord repair

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