Atopic dermatitis is a common skin disorder in dogs. This study was aimed to determine the effect of eco enzyme on dogs suffering from atopic dermatitis in terms of changes in total erythrocytes, hemoglobin levels, hematocrit values and erythrocyte indices. Two groups of dogs with atopic dermatitis were used in this study: concentration A (three dogs) were bathed with 10% concentration of eco enzyme at intervals of three days, and concentration B (two dogs) were bathed with 10% concentration of eco enzyme at intervals of three days, then continued a week later with eco enzyme concentration of 2% at intervals once a week. Both groups were given treatment for five weeks. Hematology data were analyzed through tiered pattern analysis of variance followed by Duncan’s multiple range test using SPSS which showed that the concentration of eco enzyme and the duration of observation (weeks) had no significant effect on total erythrocytes, hemoglobin, hematocrit, MCH and MCHC, although they influenced MCV. Also, there was a real interaction between the concentration of eco enzyme and the duration of observation at MCH. It can be concluded that therapy with eco enzyme has not been able to improve total erythrocytes, hemoglobin, hematocrit, MCH and MCHC, but has been able to improve MCV in dogs with atopic dermatitis.