Production and characterization of exopolysaccharides in mycelial culture ofCordyceps sinensisfungus Cs-HK1 with different carbon sources

Chen, Xia; Wu, Jianyong; Gui, Xiaoting

doi:10.1016/j.cjche.2015.06.016

Cited by 17 publications

(9 citation statements)

References 30 publications

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“…The monosaccharides Glc, Gal, and Man were found in all EPS samples, with Glc being the major component (50–75% of total). The addition of Gal or Man to the culture medium resulted in no notable increase (in some cases, even a slight decrease) in their content in EPS [ 84 ].…”

Section: Factors Regulating Mushroom Polysaccharide Synthesismentioning

confidence: 99%

Bioactive Mushroom Polysaccharides: A Review on Monosaccharide Composition, Biosynthesis and Regulation

Wang

et al. 2017

Molecules

152

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Mushrooms are widely distributed around the world and are heavily consumed because of their nutritional value and medicinal properties. Polysaccharides (PSs) are an important component of mushrooms, a major factor in their bioactive properties, and have been intensively studied during the past two decades. Monosaccharide composition/combinations are important determinants of PS bioactivities. This review summarizes: (i) monosaccharide composition/combinations in various mushroom PSs, and their relationships with PS bioactivities; (ii) possible biosynthetic pathways of mushroom PSs and effects of key enzymes on monosaccharide composition; (iii) regulation strategies in PS biosynthesis, and prospects for controllable biosynthesis of PSs with enhanced bioactivities.

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Section: Factors Regulating Mushroom Polysaccharide Synthesismentioning

confidence: 99%

Bioactive Mushroom Polysaccharides: A Review on Monosaccharide Composition, Biosynthesis and Regulation

Wang

et al. 2017

Molecules

152

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“…In this work, it was observed that different carbon sources do affect the yield or structure of EPS. As studied by Chen et al [20], who found different carbon source may influence the EPS yield of Cordyceps sinensis Cs-HK1. Their results indicated that the addition of galactose improved the total yield of EPS in fermentation culture while mannose could increase the content of glucose in EPS.…”

Section: Discussionmentioning

confidence: 94%

“…Wu et al also reported similar results when different carbon source were used to produce polysaccharide by Cordyceps sinensis fungus Cs-HK1. The glucose contents of EPS were 76% with the addition of mannose, 59% with galactose, compared with 62% with glucose only [20]. Sutherland stated that some enzymes should be involved in EPS biosynthesis [21].…”

Section: Discussionmentioning

confidence: 99%

Production, structural characterization, and antiproliferative activity of exopolysaccharide produced by Scleroderma areolatum Ehrenb with different carbon source

Jia

Huang

et al. 2019

Braz J Microbiol

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The effects of different three carbon sources, that is, glucose, fructose, and sucrose, on production, molecular properties and antiproliferative activity of exopolysaccharide (EPS), were evaluated in the submerged culture of Scleroderma areolatum Ehrenb. Among carbon sources examined, the addition of sucrose maximizes the mycelia production, while fructose could maximize the EPS yield. Although the predominant carbohydrate compositions identified were gluconic acid and mannose, the monosaccharide composition of EPSs was also different significantly. FT-IR spectral analysis revealed there was no significant difference among the prominent characteristic groups in three EPSs. The molecular weight of EPSs was also affected by carbon source, being generally lower compared with that with glucose. However, all EPSs molecule existed as nearly globular shape form in aqueous solution. The variation of carbon sources also affected antiproliferative activity examined in vitro using cell proliferation assay. Fructose was optimal carbon source giving higher antiproliferative activity probably due to the relatively high contents of xylose in the EPS with low molecular weight.

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“…Subsequently, to further development, the resulting liquid culture (2.5 mL) was transformed to a 250 mL flask fermentation culture medium and incubated for 5, 6,7 d at 25°C on a shaking table at a speed of 120 rpm [5][6][7][8]. We set up three groups parallel to avoid the occurrence of contingency and named them group 1, group 2, group 3.…”

Section: B Liquid Culturementioning

confidence: 99%

Using A New Mushroom Shake Flask Culture Device to Develop Suillellus Luridus

Hu¹,

Duan²,

You³

et al. 2017

Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)

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Abstract-A new shake flask culture device was introduced in this paper, whose main part was comprised with mouth, body and bottom. Besides, it also included cork; pump; small bacteria filter and etc. So, the utility model had the advantages of simple structure, artful design, and it was more convenient to use. Compared with the ordinary shake flask, it solved the problem of using shaking table, occupying too much space, and avoided the low yield of mycelium. Furthermore, the culture success rate was significantly higher than that of the shaking table culture. This device can also prevent the sealing gauze from being easily polluted by medium in the fermentation process, ensuring good ventilation from fermentation flask and avoiding pollution at the same time. Suillellus luridus were cultured by the two kinds of fermentation devices respectively, and the highest mycelia biomass from the new shake flask culture device could achieve 2.32 g/L, while that of the normal fermentation device only could achieve 2.11 g/L.

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Production and characterization of exopolysaccharides in mycelial culture ofCordyceps sinensisfungus Cs-HK1 with different carbon sources

Cited by 17 publications

References 30 publications

Bioactive Mushroom Polysaccharides: A Review on Monosaccharide Composition, Biosynthesis and Regulation

Bioactive Mushroom Polysaccharides: A Review on Monosaccharide Composition, Biosynthesis and Regulation

Production, structural characterization, and antiproliferative activity of exopolysaccharide produced by Scleroderma areolatum Ehrenb with different carbon source

Using A New Mushroom Shake Flask Culture Device to Develop Suillellus Luridus

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