Production and characterization of Hantaan virus-like particles from baculovirus expression system

Dai, Xiaoxia; Jian, Can; Na, Li; Li, Dexin

doi:10.1016/j.bej.2019.107373

Cited by 3 publications

(4 citation statements)

References 25 publications

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“…A baculovirus expression system was also used for the development of a combined genetic engineering vaccine for Porcine Circovirus type 2 and Mycoplasma hyopneumoniae (Tao et al, 2019). In one study, Hantaan virus-like particles were successfully produced by co-expressing Hantaan virus nucleocapsid (N) protein and glycoproteins (Gn and Gc) in Sf9 cells for vaccine studies, and it was shown that the purified VLPs provided protection from virus challenge in mice (Dai et al, 2019).…”

Section: Insect Cellsmentioning

confidence: 99%

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Tripathi

Shrivastava

2019

Front. Bioeng. Biotechnol.

400

243

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Infectious diseases, along with cancers, are among the main causes of death among humans worldwide. The production of therapeutic proteins for treating diseases at large scale for millions of individuals is one of the essential needs of mankind. Recent progress in the area of recombinant DNA technologies has paved the way to producing recombinant proteins that can be used as therapeutics, vaccines, and diagnostic reagents. Recombinant proteins for these applications are mainly produced using prokaryotic and eukaryotic expression host systems such as mammalian cells, bacteria, yeast, insect cells, and transgenic plants at laboratory scale as well as in large-scale settings. The development of efficient bioprocessing strategies is crucial for industrial production of recombinant proteins of therapeutic and prophylactic importance. Recently, advances have been made in the various areas of bioprocessing and are being utilized to develop effective processes for producing recombinant proteins. These include the use of high-throughput devices for effective bioprocess optimization and of disposable systems, continuous upstream processing, continuous chromatography, integrated continuous bioprocessing, Quality by Design, and process analytical technologies to achieve quality product with higher yield. This review summarizes recent developments in the bioprocessing of recombinant proteins, including in various expression systems, bioprocess development, and the upstream and downstream processing of recombinant proteins.

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Section: Insect Cellsmentioning

confidence: 99%

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Tripathi

Shrivastava

2019

Front. Bioeng. Biotechnol.

400

243

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“…BVs and ODVs are responsible for cell and oral infections, respectively, and they are widely used as high-efficiency expression vectors and biological pesticides because of their high safety, environmental friendliness, and simplicity of genetic manipulation [3][4][5]. Baculoviral vectors have been widely used in the fields of antigen presentation, gene therapy, and DNA vaccines [6,7]. Based on the baculovirus display system (BDS), a target protein is displayed on the surface of a baculovirus capsule and cell membrane with high efficiency and activity using a co-expression technology [8].…”

Section: Introductionmentioning

confidence: 99%

Impact of Molecular Modification on the Efficiency of Recombinant Baculovirus Vector Invasion to Mammalian Cells and Its Immunogenicity in Mice

Zheng

Pan

Wang

et al. 2022

Viruses

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The baculovirus display system (BDS), an excellent eukaryotic surface display technology that offers the advantages of safety, efficiency, and economy, is widely used in biomedicine. A previous study using rBacmid-Δgp64-ires-gp64 expressed in low copy numbers of the gp64 gene achieved high-efficiency expression and co-display of three fluorescent proteins (GFP, YFP, and mCherry). However, low expression of GP64 in recombinant baculoviruses also reduces the efficiency of recombinant baculovirus transduction into mammalian cells. In addition, the baculovirus promoter has no expression activity in mammalian cells and thus cannot meet the application requirements of baculoviral vectors for the BDS. Based on previous research, this study first determined the expression activity of promoters in insect Spodoptera frugiperda 9 cells and mammalian cells and successfully screened the very early promoter pie1 to mediate the co-expression of multiple genes. Second, utilizing the envelope display effect of the INVASIN and VSVG proteins, the efficiency of transduction of recombinant baculovirus particles into non-host cells was significantly improved. Finally, based on the above improvement, a recombinant baculovirus vector displaying four antigen proteins with high efficiency was constructed. Compared with traditional BDSs, the rBacmid-Δgp64 system exhibited increased display efficiency of the target protein by approximately 3-fold and induced an approximately 4-fold increase in the titer of serum antibodies to target antigens in Bal B/c mice. This study systematically explored the application of a new multi-gene co-display technology applicable to multi-vaccine research, and the results provide a foundation for the development of novel BDS technologies.

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“…Since its development in the early 1980s, the BEVS has experienced an unprecedented evolution 1 and is nowadays considered a robust technology to produce high levels of recombinant proteins in short times 2 . A variety of products have been produced with this system, ranging from very simple recombinant proteins 3 to more complex products including adeno‐associated virus vectors 4 and virus‐like particles (VLPs) 5 …”

Section: Introductionmentioning

confidence: 99%

“…2 A variety of products have been produced with this system, ranging from very simple recombinant proteins 3 to more complex products including adeno-associated virus vectors 4 and virus-like particles (VLPs). 5 VLPs are self-assembling nanoparticles made of one or several structural proteins of the native virus. These nanoparticles closely resemble the original virus structure but, unlike wild-type viruses, are devoid of viral genetic material.…”

Section: Introductionmentioning

confidence: 99%

Bioprocess characterization of virus‐like particle production with the insect cell baculovirus expression system at nanoparticle level

Puente‐Massaguer

Strobl

Grabherr

et al. 2022

J of Chemical Tech & Biotech

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Background. Virus-like particles (VLPs) are a multivalent platform showing great promise for the development of vaccines, gene therapy, diagnostic, and drug delivery approaches. Particularly, HIV-1 Gag VLPs provide a robust and flexible scaffold for the presentation of a variety of antigens. The insect cell baculovirus expression vector system (BEVS) is nowadays one of the reference systems to produce these complex nanoparticles, but information about VLP quality, quantity, stability, as well as cell performance is scarce, especially at bioreactor scale. Results. VLPs produced in the reference High Five and Sf9 insect cell lines share similar physicochemical properties, with VLPs produced in Sf9 cells showing lower levels of double stranded DNA and protein contaminants, and a higher degree of VLP assembly. Besides VLPs, other nanoparticle populations are divergently encountered in each cell line. Hi5 supernatants contain a higher load of extracellular vesicles, while Sf9 supernatants exhibit higher concentrations of baculovirus particles. Similar titers are achieved when comparing Gag to Gag-eGFP VLP production, with the size of most of the nanoparticles produced comprised at the 150-250 nm range. Eventually, Gag VLP production in a 2 L stirred tank bioreactor is successfully demonstrated, validating bioprocess transference to the final product candidate. Conclusions. This work provides two potentially valuable strategies for the production of HIV-1 Gag VLPs and a detailed analysis of the different nanoparticle populations produced.

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Production and characterization of Hantaan virus-like particles from baculovirus expression system

Cited by 3 publications

References 25 publications

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Impact of Molecular Modification on the Efficiency of Recombinant Baculovirus Vector Invasion to Mammalian Cells and Its Immunogenicity in Mice

Bioprocess characterization of virus‐like particle production with the insect cell baculovirus expression system at nanoparticle level

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