Production and Characterization of Indole Acetic Acid of Endophytic Bacteria isolated from Kalanchoe pinnata (Lam.) in Optimized Media

Abstract

Objectives: To optimize, quantify and characterize indole acetic acid (IAA) production from endophtic bacteria isolated from the leaves of Kalanchoe pinnata (Lam.). Methods: The selected endophytic bacteria are identified by biochemical, morphological and 16SrRNA sequencing analysis. Endophytes are screened for IAA using Salkowski reagent and media optimization is carried out for maximum IAA yield and quantification was done with UV Spectrophotometer at 30 and 120 minutes. The mean ± standard error of each sample for three triplicates is calculated from mean value of the samples followed by normal distribution. The characterization of IAA was studied with ethyl acetate extract of endophytes using TLC and HPLC. Findings: The selected endophytes are identified as Bacillus thuringiensis, Bacillus paranthracis, Staphylococcus xylosus and Bacillus cereus. Optimized yeast malt dextrose (YMD) broth containing mannitol, ammonium nitrate, and 0.2% of L-tryptophan at pH 8 and 30• C temperature