Production and characterization of monoclonal antibodies against dog immunoglobulin isotypes

Production and characterization of monoclonal antibodies specific for Pseudorabies virus

“…The literature data indicate that the quantities of immunoglobulin from 1 ng/mL to 50 ng/mL were sufficient to establish reactions with appropriate antigens. These quantities are smaller than those necessary for ELISA assay (Arce et al, 2002;Sulimenko and Draber, 2004). Our experiments with both PrV viral antigens bonded to PVDF membranes showed minimal quantities of bound immunoglobulin (2.58 to 165 ng/mm 2 ) and comply with literature data (Fleming and Pen, 1988).…”

“…Therefore, specific monoclonal antibodies (MAbs), if used together with other techniques, would be an alternative tool for a rapid and accurate diagnostic test for the early detection of Aujeszky's disease. (Eloit et al, 1989;Qvist et al, 1990;Zsak et al, 1992;Morenkov et al, 1999;Arce et al, 2002).…”

Two cDNAs coding for the porcine CD51 (αv) integrin subunit: Cloning, expression analysis, adhesion assays and chromosomal localization

“…5H2 monoclonal antibody was produced using previously described immunization and cell fusion procedures (Arce et al, 2002). Briefly, female BALB/c mice were immunized with 50 μg of rpCD51.…”

Molecular characterization and expression analysis of the gene coding for the porcine β3 integrin subunit (CD61)

“…AJ2G11 monoclonal antibody was produced using previously described immunization and cells fusion procedures (Arce et al, 2002). Briefly, female BALB/c mice were immunized with 50 μg of rpCD61.…”