Production and Characterization of Monoclonal Antibodies Against the VP7 Protein of Epizootic Hemorrhagic Disease Virus

Armillotta, Gisella; Febo, Tiziana Di; Ulisse, Simonetta; Laguardia, C.; Iorio, Mariangela; Krasteva, Ivanka; Tittarelli, Manuela; Mercante, Maria Teresa; Luciani, Mirella

doi:10.1089/mab.2021.0019

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…Thus, the absence of immunopathology may be also an indicative of virus neutralization induced by the vaccine. Shared B- and T-cell epitopes and cross-reactive responses between BTV and EHDV are known 85 , 86 . We evaluated the protective potential against EHDV of a recombinant MVA-based vaccine against BTV.…”

Section: Discussionmentioning

confidence: 99%

IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation

Jiménez-Cabello,

Utrilla-Trigo,

Benavides-Silván

et al. 2024

Int. J. Biol. Sci.

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Epizootic hemorrhagic disease (EHD), caused by Epizootic hemorrhagic disease virus (EHDV), is an emerging and severe livestock disease. Recent incursion and distribution of EHDV in Europe have outlined the emerging character of EHD. Despite its worldwide impact, numerous knowledge gaps exist. A range of inconveniences restricts utilization of natural hosts of EHDV. Here, we show that adult mice deficient in type I IFN receptor (IFNAR(-/-)) are highly susceptible to EHDV-6 and EHDV-8 infection when the virus is administered subcutaneously. Disease was characterized by ruffled hair, reluctance to move, dehydration and conjunctivitis, with viraemia detected from day 5 post-infection. A deeper characterization of EHDV-8 infection showed viral replication in the lung, liver, spleen, kidney, testis and ovaries. Importantly, increased expression levels of pro-inflammatory cytokines IL-1β, IL-6 and CXCL2 were observed in spleen after EHDV-8 infection. Furthermore, IFNAR(-/-) adult mice immunized with a EHDV-8 inactivated vaccine elicited neutralizing antibodies specific of EHDV-8 and full protection against challenge with a lethal dose of this virus. This study also explores the possibilities of this animal model for study of BTV and EHDV coinfection. In summary, the IFNAR(-/-) mouse model faithfully recapitulates EHD and can be applied for vaccine testing, which can facilitate progress in addressing the animal health challenge posed by this virus.

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Section: Discussionmentioning

confidence: 99%

IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation

Jiménez-Cabello,

Utrilla-Trigo,

Benavides-Silván

et al. 2024

Int. J. Biol. Sci.

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“…A recent article highlighted the existence of shared B- and T-cell epitopes between the sequence of the EHDV and BTV VP7 and VP5 proteins, by in silico analysis [ 179 ]. Further, monoclonal antibodies directed against VP7 of EHDV also reacted against BTV VP7, which highlights the presence of discontinuous epitopes shared between these two orbiviruses [ 180 ]. Nonetheless, without dismissing this approach, experimental infection of EHDV-2 convalescent WTD with BTV-10, or infection of BTV convalescent cattle with EHDV-7, proved that previous exposure to one orbivirus did not impair viral replication of the other one [ 118 , 144 ].…”

Section: Classic and Novel Vaccine Approaches Against Ehdvmentioning

confidence: 99%

Epizootic Hemorrhagic Disease Virus: Current Knowledge and Emerging Perspectives

Jiménez-Cabello¹,

Utrilla-Trigo²,

Lorenzo³

et al. 2023

Microorganisms

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Epizootic Hemorrhagic Disease (EHD) of ruminants is a viral pathology that has significant welfare, social, and economic implications. The causative agent, epizootic hemorrhagic disease virus (EHDV), belongs to the Orbivirus genus and leads to significant regional disease outbreaks among livestock and wildlife in North America, Asia, Africa, and Oceania, causing significant morbidity and mortality. During the past decade, this viral disease has become a real threat for countries of the Mediterranean basin, with the recent occurrence of several important outbreaks in livestock. Moreover, the European Union registered the first cases of EHDV ever detected within its territory. Competent vectors involved in viral transmission, Culicoides midges, are expanding its distribution, conceivably due to global climate change. Therefore, livestock and wild ruminants around the globe are at risk for this serious disease. This review provides an overview of current knowledge about EHDV, including changes of distribution and virulence, an examination of different animal models of disease, and a discussion about potential treatments to control the disease.

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Production and Characterization of Monoclonal Antibodies Against the VP7 Protein of Epizootic Hemorrhagic Disease Virus

Cited by 2 publications

References 17 publications

IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation

IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation

Epizootic Hemorrhagic Disease Virus: Current Knowledge and Emerging Perspectives

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