Production and characterization of monoclonal antibodies for aflatoxin B1

Devi, K T; Mayo, M. A.; Reddy, Karen L.; Delfosse, Philippe; Reddy, Gopal; Reddy, S. V.; Reddy, D. V. R.

doi:10.1046/j.1472-765x.1999.00685.x

Cited by 60 publications

(32 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The resulting monoclonal antibodies all show similar selectivities. The affinity to the aflatoxins usually follows the order AFB1 > AFG1 > AFB2 > AFG2 [10][11][12][13]. For this type of conjugate the cross-reactivity pattern is in accordance with the hapten structure shown in Figure 2.…”

Section: Introductionmentioning

confidence: 69%

Novel Aflatoxin Derivatives and Protein Conjugates

et al. 2007

View full text Add to dashboard Cite

Aflatoxins, a group of structurally related mycotoxins, are well known for their toxic and carcinogenic effects in humans and animals. Aflatoxin derivatives and protein conjugates are needed for diverse analytical applications. This work describes a reliable and fast synthesis of novel aflatoxin derivatives, purification by preparative HPLC and characterisation by ESI-MS and one-and two-dimensional NMR. Novel aflatoxin bovine serum albumin conjugates were prepared and characterised by UV absorption and MALDI-MS. These aflatoxin protein conjugates are potentially interesting as immunogens for the generation of aflatoxin selective antibodies with novel specificities.

show abstract

Section: Introductionmentioning

confidence: 69%

Novel Aflatoxin Derivatives and Protein Conjugates

et al. 2007

View full text Add to dashboard Cite

show abstract

“…All other chemicals were of reagent grade or chemically pure. Highly specific polyclonal antibodies for AFB1 were purchased from International Crop Research Institute for the Semi Arid Tropics, Patancheru, India (Devi et al 1999). …”

Section: Materials For Elisamentioning

confidence: 99%

Genetic variability of aflatoxin B1 producing Aspergillus flavus strains isolated from discolored rice grains

Reddy

Kumar

Reddy

et al. 2008

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Twenty-two aflatoxin B 1 (AFB1) producing Aspergillus flavus strains were isolated from 1,200 discolored rice grain samples collected from 20 states across India and tested their potential to produce AFB1 on different agar media. Further these isolates were characterized through randomly amplified polymorphic DNA method. All the strains of A. flavus were produced AFB1 on yeast extract sucrose agar media and none of the strains on A. flavus and A. parasiticus agar. Among the 22 strains, two strains from Tamil Nadu (DRAf 009) and Maharashtra (DRAf 015) produced high amount of AFB1 in all the media tested. To assess the genetic variability in A. flavus, the isolates were analyzed by using random amplified polymorphic DNA markers. Isolates showed 17-80% similarity with standard culture of A. flavus (MTCC 2799).

show abstract

“…Some sensitive antibodies were produced for the determination of AFB 1 in different agricultural products (Candlish et al 1987;Devi et al 1999;Lee 2004;Kolosova et al 2006;Wang and Liu 2006;Li et al 2009). In addition, some ELISA methods were developed for the determination of AFM 1 in dairy products (Magliulo et al 2005;Rosi et al 2007;Anfossi et al 2008;Pei et al 2009;Zhang et al 2009;Guan et al 2011).…”

Section: Sample Preparation and Assay Validationmentioning

confidence: 99%

“…The lateral flow strips are simple, rapid, and qualitative methods, while ELISA was a sensitive and semi-quantitative method. During the last two decades, several ELISA methods and other immunoassays were developed for the determination of AFB 1 or AFM 1 (Candlish et al 1987;Devi et al 1999;Magliulo et al 2005;Wang and Liu 2006;Anfossi et al 2008;Li et al 2009;He et al 2012;Puiu et al 2012;Jinap et al 2012;Rossi et al 2012). The aim of our study was generation of a broad-specificity MAb, enabling development of a sensitive and reliable immunoassay capable of detecting a broader range of aflatoxins.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Aflatoxin B1 and Aflatoxin M1 in Food Matrices by Enzyme-Linked Immunosorbent Assay

et al. 2012

View full text Add to dashboard Cite

Aflatoxins are a class of highly toxic chemical contaminants occurring in food. Consumption of aflatoxincontaminated food can lead to harmful effects on human health. A rapid and reliable analysis of aflatoxins in food is crucial. In this study, we generated a broad-specific monoclonal antibody (MAb) 3 C10 against aflatoxin B 1 (AFB 1 ). The MAb 3 C10 binds specifically to AFB 1 and AFM 1 and has a IC 50 of 0.13 μg L −1 for AFB 1 and 0.16 μg L −1 for AFM 1 . Furthermore, the MAb showed high cross-reactivity to AFB 2 , AFG 1 , and AFG 2 . To enable simultaneous AFB 1 and AFM 1 detection in different food matrices, an indirect competitive enzyme-linked immunosorbent assay (icELISA) based on MAb 3 C10 has been developed and optimized. In addition, the extraction methods of different food matrices (peanut, corn, soybean, wheat flour, rice, soy sauce, vinegar, wine, raw milk, pure milk, skimmed milk, and yogurt) were established. The average recovery ranged from 73 to 121 %, with relative standard deviation values less than 15 %. The limit of detection was 0.52+0.36 μg kg −1 (mean+3SD) for AFB 1 in eight agricultural products and 0.031+0.015 μg kg −1 (mean+3SD) for AFM 1 in four dairy products. The sensitivity of icELISA was below the limit set by the European Commission for aflatoxin detection in different food matrices and similar to LC-MS/MS method. We demonstrate a rapid, simple, and reliable method for simultaneous screening of AFB 1 and AFM 1 in different food matrices.

show abstract

Production and characterization of monoclonal antibodies for aflatoxin B1

Cited by 60 publications

References 8 publications

Novel Aflatoxin Derivatives and Protein Conjugates

Novel Aflatoxin Derivatives and Protein Conjugates

Genetic variability of aflatoxin B1 producing Aspergillus flavus strains isolated from discolored rice grains

Simultaneous Determination of Aflatoxin B1 and Aflatoxin M1 in Food Matrices by Enzyme-Linked Immunosorbent Assay

Contact Info

Product

Resources

About