Production and characterization of murine monoclonal anti-human DNase II antibodies, and their use for immunoaffinity purification of DNase II from human liver and urine

Nakajima, Tamiko; Yasuda, Toshihiro; Takeshita, Hitoshi; Mori, Shinjiro; Mogi, Kouichi; Kaneko, Yasushi; Nakazato, Emiko; Kishi, Koichiro

doi:10.1016/s0304-4165(02)00191-5

Cited by 2 publications

(1 citation statement)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several different molecular weights that have been reported for human DNase II differ between the reported data. These have been listed as 45 kDa [18], [19] and 38 kDa [20] forms in human cell lines, and a 32 kDa protein in the liver and urine [21]. Purified porcine DNase II was determined by gel filtration to have a molecular weight of 45 kDa, but SDS-PAGE showed molecular weights of 35 and 10 kDa [22].…”

Section: Introductionmentioning

confidence: 99%

Biogenesis and Proteolytic Processing of Lysosomal DNase II

et al. 2013

View full text Add to dashboard Cite

Deoxyribonuclease II (DNase II) is a key enzyme in the phagocytic digestion of DNA from apoptotic nuclei. To understand the molecular properties of DNase II, particularly the processing, we prepared a polyclonal antibody against carboxyl-terminal sequences of mouse DNase II. In the present study, partial purification of DNase II using Con A Sepharose enabled the detection of endogenous DNase II by Western blotting. It was interesting that two forms of endogenous DNase II were detected – a 30 kDa form and a 23 kDa form. Neither of those forms carried the expected molecular weight of 45 kDa. Subcellular fractionation showed that the 23 kDa and 30 kDa proteins were localized in lysosomes. The processing of DNase II in vivo was also greatly altered in the liver of mice lacking cathepsin L. DNase II that was extracellularly secreted from cells overexpressing DNase II was detected as a pro-form, which was activated under acidic conditions. These results indicate that DNase II is processed and activated in lysosomes, while cathepsin L is involved in the processing of the enzyme.

show abstract