Production and characterization of murine monoclonal antibody against synthetic peptide of CD34

Maleki, Leili Aghebati; Majidi, Jafar; Baradaran, Behzad; Abdolalizadeh, Jalal; Akbari, Aliakbar Movassaghpour

doi:10.3233/hab-130265

Cited by 14 publications

(7 citation statements)

References 15 publications

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“…Peptide Abs to cell surface markers are often used at fluorescent conjugates in fluorescence microscopy for IHC or flow cytometry (FC) for cells in suspension [63][64][65]143].…”

Section: Applicationsmentioning

confidence: 99%

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Peptides, Antibodies, Peptide Antibodies and More

Trier

Hansen

Houen

2019

IJMS

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The applications of peptides and antibodies to multiple targets have emerged as powerful tools in research, diagnostics, vaccine development, and therapeutics. Antibodies are unique since they, in theory, can be directed to any desired target, which illustrates their versatile nature and broad spectrum of use as illustrated by numerous applications of peptide antibodies. In recent years, due to the inherent limitations such as size and physical properties of antibodies, it has been attempted to generate new molecular compounds with equally high specificity and affinity, albeit with relatively low success. Based on this, peptides, antibodies, and peptide antibodies have established their importance and remain crucial reagents in molecular biology.

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“…Peptide Abs to cell surface markers are often used at fluorescent conjugates in fluorescence microscopy for IHC or flow cytometry (FC) for cells in suspension [63][64][65]143].…”

Section: Applicationsmentioning

confidence: 99%

“…Peptide Abs are used for numerous research purposes as well. For example, peptide Abs are often used for detecting cell surface markers, e.g., CD3, CD8, CD14, CD20, and CD34 in FC [63][64][65]143].…”

Section: Applicationsmentioning

confidence: 99%

Peptides, Antibodies, Peptide Antibodies and More

Trier

Hansen

Houen

2019

IJMS

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“…Therapeutic antibodies are significant drugs for the treatment of autoimmune diseases and infections. Furthermore, antibodies are important substances in diagnostic and medical researches including therapeutic effects [9,10] they are a combination of monoclonal Abs produced against different epitopes of antigens and have great avidity to polyvalent antigens [11,12]. Sheeppox virus, Goatpox viruses, and Lumpy skin disease viruses are surprisingly alike to one another, sharing 97% nucleotide identity and studies also revealed that Sheeppox virus and Goatpox viruses share about 96% nucleotide identity over their entire length [13].…”

Section: Discussionmentioning

confidence: 99%

Production and assessment of polyclonal antibody against the whole virion of Goatpox virus and sheeppox virus in the rabbit

Hurisa¹,

Jia²,

Chen³

et al. 2019

Preprint

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Background: Antibodies have emerged as essential tools of biomedical researches and are of great commercial and medical values. They are the fastest growing product segments of the pharmaceutical industry. Polyclonal antibodies are antibodies that secreted by different B cell lineages within the body. In this study production of polyclonal antibody against Goatpox and Sheeppox virion in rabbit was performed and subsequently; it will be used as the development of ELISA for detection of poxvirus in different species of animals. Results: After 0.5mg/ml of the whole virion of the poxvirus was injected subcutaneously at multiple sites, the blood was collected at an interval of 14 days and antibody titration was conducted. GTPV A27 antigen was coated and indirect ELISA method was used for the titration of antibody then the mean of OD values for positive and negative results were analyzed by Microsoft excel window 7. The blood was collected and serum was prepared for IgG purification. The IgG was purified by Ammonium sulfate precipitation method which was dialyzed against 5lire PBS overnight at +4oc. The protein concentration was determined spectrophotometrically at 280nm wavelength and estimated as 2.29µg/µl and 2.18µg/µl against Goatpox virus and sheeppox virus respectively. Protein bands were checked by SDS PAGE and the molecular weights of 67kDa and 25kDas were estimated for IgG. similarly, antigen-antibody detection was checked by western blot. Conclusions: Based on the results of this study, the virion of Goatpox virus and Sheeppox virus is strong enough to produce antibody to develop ELISA kit for detection of poxviruses by coating GPTV A27 antigen. This study is the first to be conducted for the production of antibody from virions of Goatpox and sheeppox virus which was tittered by coating GTPV A27 antigen.

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“…The mAbs have several applications including Cancer Immunotherapy, 91 , 94 Bone Marrow and Organ Transplantation, 95 , 96 Protein Purification, 97 - 99 Disease Diagnosis in immunoassay tests including enzyme-linked immune sorbent assay (ELISA), western blotting, immunohistochemistry and immunofluorescence tests. 100 , 101 Our team has been developed the mAbs against a wide range of antigens including TNF-α, 102 CD20, 103 CD34, 104 , 105 receptor tyrosine kinase-like orphan receptor proteins, 106 human epidermal growth factor receptor, 107 heat shock protein 70, 108 vascular endothelial growth factor receptor 2, 77 , 78 human IgG and IgA. 109 , 110 …”

Section: Monoclonal Antibodiesmentioning

confidence: 99%

PCSK9: A Key Target for the Treatment of Cardiovascular Disease (CVD)

et al. 2020

Self Cite

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Proprotein convertase subtilisin/kexin type 9 (PCSK9), as a vital modulator of low-densitylipoprotein cholesterol (LDL-C) , is raised in hepatocytes and released into plasma where it bindsto LDL receptors (LDLR), leading to their cleavage. PCSK9 adheres to the epidermal growthfactor-like repeat A (EGF-A) domain of the LDLR which is confirmed by crystallography. LDLRexpression is adjusted at the transcriptional level through sterol regulatory element bindingprotein 2 (SREBP-2) and at the post translational stages, specifically through PCSK9, and theinducible degrader of the LDLR PCSK9 inhibition is an appealing new method for reducing theconcentration of LDL-C. In this review the role of PCSK9 in lipid homeostasis was elucidated, theeffect of PCSK9 on atherosclerosis was highlighted, and contemporary therapeutic techniquesthat focused on PCSK9 were summarized. Several restoration methods to inhibit PCSK9 havebeen proposed which concentrate on both extracellular and intracellular PCSK9, and theyinclude blockage of PCSK9 production by using gene silencing agents and blockage of it’sbinding to LDLR through antibodies and inhibition of PCSK9 autocatalytic processes by tinymolecule inhibitors.

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Production and characterization of murine monoclonal antibody against synthetic peptide of CD34

Abstract: This antibody is highly specific and functional in biomedical applications such as ELISA, flowcytometry, immunofluorescence, and western blot assays.

Cited by 14 publications

References 15 publications

Peptides, Antibodies, Peptide Antibodies and More

Peptides, Antibodies, Peptide Antibodies and More

Production and assessment of polyclonal antibody against the whole virion of Goatpox virus and sheeppox virus in the rabbit

PCSK9: A Key Target for the Treatment of Cardiovascular Disease (CVD)

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