Production and characterization of novel self-assembling biosurfactants from Aspergillus flavus

Abstract

Aim This work was conducted to produce, purify and characterize biosurfactants from Aspergillus flavus AF612 isolated from citrus fruit. Methods and results Biosurfactant named ‘Uzmaq’ was isolated from A. flavus AF612. The chemical characterization of the biosurfactant was conducted. Biosurfactant Uzmaq produced by A. flavus, was composed of methoxy phenyl oxime glycosides. Two molecular forms of the biosurfactant, Uzmaq‐A and Uzmaq‐B were isolated. Biological properties (antifungal activity) were evaluated. The fractions of the biosurfactant were isolated and their surface properties were analysed. Uzmaq‐A and Uzmaq‐B had critical micelle concentration (CMC) around 170 and 80 mg l−1, and lowered surface tension of water up to 20 and 25 m Nm−1 respectively. The biosurfactants were stable at pH 3–12 and temperature up to 80°C. Growth and biosurfactant production kinetics were also analysed. Conclusion Novel biosurfactant Uzmaq was produced from A. flavus, which was composed of methoxy phenyl oxime glycosides. The surface activity of Uzmaq was better than the maximum values of synthetic chemical surfactants. The biosurfactant showed antifungal activity and self‐assembling properties. Significance and Impact of the Study Aspergillus flavus AF612 can be used for commercial production of Uzmaq that may be employed for controlled drug release applications and bioremediation.

Eco-Friendly Bioemulsifier Production by Mucor circinelloides UCP0001 Isolated from Mangrove Sediments Using Renewable Substrates for Environmental Applications

“…Emulsification activities (EA) obtained by filamentous fungi are considered to correlate strongly with bioemulsifier production [23]. In this sense, the response surface method (RSM) was used to construct a model.…”

SIMULTANEOUS PRODUCTION OF BIOSURFACTANTS AND LIPASES FROM Aspergillus niger AND OPTIMIZATION BY RESPONSE SURFACE METHODOLOGY AND DESIRABILITY FUNCTIONS

“…The biosurfactant production was monitored using as parameters the emulsifying activities EA w/o and EA w/o . It is also known that emulsification activities from Aspergillus strains strongly correlate with the production of biosurfactants (Castiglioni et al, 2009;Ishaq et al, 2015). The response of EA w/o was impacted negatively by the factors AG and SO, while ME was not significant (at the level of 5%).…”

Improved biosurfactant production from Aspergillus niger through chemical mutagenesis: characterization and RSM optimization

“…There are two basic strategies that are being adopted by the biotechnologists for economical biosurfactant production, which corresponds, the use of low-cost waste materials to reduce the overall substrate cost and the efficient bioprocesses, including optimized fermentation parameters involving multiple physical and nutritional factors [45]. The selection of appropriate substrate is a crucial step in the production of biosurfactant, as the substrate account for overall 10-30% cost of the experiment [45,46]. Therefore, in this context, herein, a cost-effective process was developed for the production of biosurfactant using agro-industrial raw-material (banana stalks powder) with waste-to-value approach.…”