Production and characterization of polyclonal antibody against Bengal tiger (<i>Panthera tigris tigris</i>) immunoglobulin G

Areewong, Chanakan; Sangchantip, Rinrada; Rungphattanachaikul, Surapa; Rittipornlertrak, Amarin; Fhaikruae, Itsarapan; Wongkalasin, Waroot; Nomsiri, Ruksiri; Boontong, Pinich; Vongchan, Preeyanat; Sthitmatee, Nattawooti

doi:10.1080/09712119.2019.1629937

Cited by 3 publications

(3 citation statements)

References 54 publications

(57 reference statements)

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“…Purified Bengal tiger IgG was obtained from a previous study [32]. New Zealand white rabbits (Mlac: NZW, National Laboratory Animal Center, Mahidol University, Nakhon Pathom, Thailand) were weekly subcutaneously immunized with purified tiger IgG (1 mg/dose.1 ml) formulated with Montanide™ ISA 206 VG (Seppic, Paris, France; 1:1 v/v, 100 μg/ml) for 4 weeks.…”

Section: Production Of Rabbit Anti-tiger Igg Polyclonal Antibodymentioning

confidence: 99%

“…New Zealand white rabbits (Mlac: NZW, National Laboratory Animal Center, Mahidol University, Nakhon Pathom, Thailand) were weekly subcutaneously immunized with purified tiger IgG (1 mg/dose.1 ml) formulated with Montanide™ ISA 206 VG (Seppic, Paris, France; 1:1 v/v, 100 μg/ml) for 4 weeks. The rising titer of rabbit anti-tiger IgG was measured using indirect ELISA [32]. Due to the blood collection of rabbits, the generalized anesthesia was done by intravenous injection of pentobarbitone sodium (Nembutal, 20 mg/kg).…”

Section: Production Of Rabbit Anti-tiger Igg Polyclonal Antibodymentioning

confidence: 99%

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Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

et al. 2020

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Background: Feline panleukopenia virus (FPV) is an etiologic pathogen of feline panleukopenia that infects all members of Felidae including tigers (Panthera tigris). Vaccinations against FPV among wild felid species have long been practiced in zoos worldwide. However, few studies have assessed the tiger immune response post-vaccination due to the absence of a serological diagnostic tool. To address these limitations, this study aimed to develop an in-house indirect enzyme-linked immunosorbent assay (ELISA) for the monitoring of tiger antibody levels against the feline panleukopenia vaccine by employing the synthesized subunit capsid protein VP2. An in-house horseradish peroxidase (HRP) conjugated rabbit anti-tiger immunoglobulin G (IgG) polyclonal antibody (HRP-anti-tiger IgG) was produced in this study and employed in the assay. It was then compared to a commercial HRP-conjugated goat anti-cat IgG (HRPanti-cat IgG). Sensitivity and specificity were evaluated using the Bayesian model with preferential conditional dependence between HRP-conjugated antibody-based ELISAs and hemagglutination-inhibition (HI) tests. Results: The posterior estimates for sensitivity and specificity of two indirect ELISA HRP-conjugated antibodies were higher than those of the HI test. The sensitivity and specificity of the indirect ELISA for HRP-anti-tiger IgG and HRP-anticat IgG were 86.5, 57.2 and 86.7%, 64.6%, respectively, while the results of the HI test were 79.1 and 54.1%. In applications, 89.6% (198/221) and 89.1% (197/221) of the tiger serum samples were determined to be seropositive by indirect ELISA testing against HRP-anti-tiger and HRP-anti-cat, respectively.

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Section: Production Of Rabbit Anti-tiger Igg Polyclonal Antibodymentioning

confidence: 99%

Section: Production Of Rabbit Anti-tiger Igg Polyclonal Antibodymentioning

confidence: 99%

Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

et al. 2020

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“…Purified Bengal tiger IgG was obtained from a previous study [32]. New Zealand white rabbits were weekly subcutaneously immunized with purified tiger IgG formulated with Montanide ™ ISA 206 VG (Seppic, Paris, France; 1:1 v/v, 100 µg/ml) for 4 weeks.…”

Section: Production Of Rabbit Anti-tiger Igg Polyclonal Antibodymentioning

confidence: 99%

Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

Areewong

Rittipornlertrak

Nambooppha

et al. 2020

Preprint

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Background: Feline panleukopenia virus (FPV) is an etiologic pathogen of feline panleukopenia that infects all members of Felidae including tigers (Panthera tigris). Vaccination against FPV among wild felid species has long been practiced in zoos worldwide. However, few studies have assessed tiger immune response post-vaccination due to the absence of a serological diagnostic tool. To address these limitations, this study aimed to develop an in-house indirect enzyme-linked immunosorbent assay (ELISA) for the monitoring of tiger antibody levels against the feline panleukopenia vaccine by employing the synthesized subunit capsid protein VP2. An in-house horseradish peroxidase (HRP) conjugated rabbit anti-tiger immunoglobulin G (IgG) polyclonal antibody (HRP-anti-tiger IgG) was produced in this study and employed in the assay. It was then compared to a commercial HRP-conjugated goat anti-cat IgG (HRP-anti-cat IgG). Sensitivity and specificity were evaluated using the Bayesian model with conditional dependence being assumed between both HRP-conjugated antibody-based ELISAs and hemagglutination-inhibition (HI) tests. Results: The posterior estimates for sensitivity and specificity of two indirect ELISA HRP-conjugated antibodies were higher than those of the HI test. The sensitivity and specificity of indirect ELISA for HRP-anti-tiger IgG and HRP-anti-cat IgG were 86.5%, 57.2% and 86.7%, 64.6%, respectively, while the results of the HI test were 79.1 and 54.1%. In applications, 89.6% (198/221) of tiger serum samples were determined to be seropositive by indirect ELISA testing. Conclusion: The results support evidence that an in-house indirect ELISA developed in this study could be used as a serological tool for the effective detection of tiger antibodies.

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A Study on the Impact of Global Warming on Wildlife

Varis

2024

ShodhKosh J. Vis. Per. Arts

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Global warming, a pressing issue of our time, is exerting a profound influence on the delicate balance of our planet's ecosystems. Among its far-reaching consequences, the impact on wildlife is particularly alarming. As temperatures rise, sea levels climb, and weather patterns become increasingly erratic, countless species are facing unprecedented challenges to their survival. One of the most significant impacts of global warming on wildlife is habitat loss. As temperatures rise, ecosystems are shifting, and many species are struggling to adapt to these changes. For example, the melting of polar ice caps is threatening the existence of polar bears, seals, and other Arctic species. Similarly, coral reefs, which are vital habitats for countless marine organisms, are bleaching and dying due to rising ocean temperatures and acidification. Climate change is also disrupting the delicate timing of biological events, such as migration and breeding. Many species rely on specific environmental cues, such as temperature and day length, to trigger these behaviors. However, as these cues shift due to climate change, animals may miss critical opportunities for feeding, breeding, or migrating, leading to population declines.

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Production and characterization of polyclonal antibody against Bengal tiger (Panthera tigris tigris) immunoglobulin G

Cited by 3 publications

References 54 publications

Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

A Study on the Impact of Global Warming on Wildlife

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