Production and characterization of somatic hybrids between Solanum tuberosum L. and S. pinnatisectum Dun.

Sarkar, Debabrata; Tiwari, Jagesh Kumar; Sharma, Sushruti; Poonam, .; Sharma, Sanjeev; Gopal, J.; Singh, B. P.; Luthra, S. K.; Pandey, S. K. : Singh; Pattanayak, Debasis

doi:10.1007/s11240-011-9993-8

Cited by 47 publications

(23 citation statements)

References 39 publications

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“…RAPD primers used in the analysis are summarized in Supplementary Table 1. The polymerase chain reaction (PCR) amplification and analysis was performed as described by Sarkar et al (2011) and Tiwari et al (2010). Inter simple sequence repeat (ISSR) analysis was performed using genomic DNA by ISSR primers summarized in Supplementary Table 1.…”

Section: Dna Analysismentioning

confidence: 99%

Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers

Tiwari

Chandel

Gupta

et al. 2013

Physiol Mol Biol Plants

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The genetic stability of in vitro propagated potato microtubers was assessed using random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. Microtubers were developed through in vitro from potato microplants using standardized protocols. The microtubers were conserved for 1 year under three different culture media and consequently microplants were regenerated for the DNA analyses. During the study, a total of 38 (10 RAPD, 11 ISSR, 12 SSR and 5 AFLP) primers produced a total of 407 (58 RAPD, 56 ISSR, 96 SSR and 197 AFLP) clear, distinct and reproducible amplicons. Cluster analysis revealed 100 % genetic similarity among the mother plant and its derivatives within the clusters by SSR, ISSR and RAPD analyses, whereas AFLP analysis revealed from 85 to 100 % genetic similarity. Dendrogram analysis based on the Jaccard's coefficient classified the genotypes into five clusters (I-V), each cluster consisting of mother plant and its derivatives. Principal component analysis (PCA) also plotted mother plant and its genotypes of each cluster together. Based on our results, it is concluded that AFLP is the best method followed by SSR, ISSR and RAPD to detect genetic stability of in vitro conserved potato microtubers. The in vitro conservation medium (T2) is a safe method for conservation of potato microtubers to produce true-to-type plans.

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Section: Dna Analysismentioning

confidence: 99%

Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers

Tiwari

Chandel

Gupta

et al. 2013

Physiol Mol Biol Plants

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“…The plant materials for microarray were developed previously via protoplasts fusion between dihaploid Solanum tuberosum 'C-13' and late blight resistant wild S. pinnatisectum (Sarkar et al, 2011). In this study, the nucleotide sequences of the 153 putative known function potato transcripts were retrieved from the potato genome browser (http://potato.plantbiology.msu.edu) for computational analyses.…”

Section: Source Of Potato Transcriptsmentioning

confidence: 99%

In silico identification of candidate microRNAs and their targets in potato somatic hybrid Solanum tuberosum (+) S. pinnatisectum for late blight resistance

Singh¹,

Tiwari²,

Rawat³

et al. 2016

Plant Omics

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We employed computational tools to identify candidate miRNAs from 153 putative known genes obtained from a previous microarray experiment by Singh et al. (2016) for late blight resistance. In this study, we identified eight miRNAs families (miR395, 821, 1030, 1510, 2673, 3979, 5021 and 5213) to the nine potato genes. Mature miRNAs located at different positions of pre-miRNAs are varied from 19 to 21 nucleotides in length. Of the total 343 published miRNAs in potato (miRBase), all identified miRNAs family are new to potato crop except miR395. In particular, these novel miRNAs were identified for pathogen-induced late blight resistance mechanism in interspecific potato somatic hybrid. Majority of the predicted target genes (Table 2) of these miRNAs are involved in different biological functions, including disease resistance proteins (NBS-LRR domains) and transcription factors families. This study offers an insight to identify potential candidate miRNAs and their targets to dissect late blight resistance mechanism in potato somatic hybrid Solanum tuberosum (+) S. pinnatisectum.

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“…In addition, to obtain late blight resistance from other genetic resources, interspecific somatic hybrids between cultivated potato and S. pinnatisectum Dun. (Sarkar et al 2011) and S. tarnii Hawkes and Hjert. (Thieme et al 2008) were produced.…”

Section: Somatic Genome Manipulation Applications To Transfer Valuablmentioning

confidence: 99%

Protoplast Technology in Genome Manipulation of Potato Through Somatic Cell Fusion

Rokka

2015

Somatic Genome Manipulation

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Production and characterization of somatic hybrids between Solanum tuberosum L. and S. pinnatisectum Dun.

Cited by 47 publications

References 39 publications

Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers

Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers

In silico identification of candidate microRNAs and their targets in potato somatic hybrid Solanum tuberosum (+) S. pinnatisectum for late blight resistance

Protoplast Technology in Genome Manipulation of Potato Through Somatic Cell Fusion

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