Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion

Abstract

Symmetric somatic hybrid plants between Gossypium hirsutum Coker 201 and G. klotzschianum were obtained through electrofusion. The fusion products were cultured in KM8P medium supplemented with 2.685 microM alpha-naphthaleneacetic acid and 0.465 microM kinetin, and the regenerated plants were morphologically, genetically, and cytologically characterized. Nuclear-DNA flow cytometric analysis revealed that the plants tested (31 of 40) had a relative DNA content close to the total DNA contents of the two parents. Subsequent genome DNA analysis using random amplified polymorphic DNA markers revealed 16 of 18 plants were true somatic hybrids. Cytological investigation of the metaphase root-tip cells of seven hybrids revealed there were 72-81 chromosomes in the hybrids, a value close to the expected 78 chromosomes. The morphology of the hybrids was distinct from that of the parents and from that of the regenerants from protoplasts of Coker 201. Somatic hybridization represents a potent and novel tool for transferring genomes of wild cottons containing economically important traits to cultivars in breeding programs. This is the first report on the regeneration of somatic hybrids via protoplast fusion in cotton.

Date Palm Cell and Protoplast Culture

“…Recently nurse culture technique has been successfully used to address the regeneration issues in recalcitrant protoplasts (Assani et al 2006;Horita et al 2002;Sun et al 2004). The number of microcalli we obtained was close to those obtained in earlier study in banana (Assani et al 2001).…”

Laccase GhLac1 Modulates Broad-Spectrum Biotic Stress Tolerance via Manipulating Phenylpropanoid Pathway and Jasmonic Acid Synthesis

“…The cell suspensions used in this experiment was derived from YZ1 embryogenic callus after two weeks subculture in dark (Sun et al, 2004). The 5-mL cell suspension cultures were treated with 5 mL of the water soluble fraction from wild type or the transgenic lines in 50 mL Erlenmeyer flasks, using the equal volume of distilled water or PGA (final concentration 0.5 mg/mL) as negative or positive control, respectively.…”

Gossypium

“…The coker varieties are now the varieties of choice for genetic transformation (Firoozabady et al 1987;Umbeck et al 1987;Rathore et al 2006). The first successful somatic hybridization through protoplast fusion was achieved between G. hirsutum Coker 201 and G. klotzschianum (Sun et al 2004) and such methodologies represent potential and novel tools for transferring the agronomically important characteristics of wild cottons to cultivated cotton in global breeding programs.…”