Production and Characterization of α-Amylase from Aspergillus niger

Characterization and optimization of extracellular enzymes production by Aspergillus niger strains isolated from date by-products

“…These results are in agreement with Varalakshmi et al [ 22 ] indicating that the best enzyme production in A. niger is obtained at room temperature both in submerged fermentation (SmF) and solid-state fermentation (SSF). Various studies reported that this parameter is very important during the enzymatic production, and they found that 30 °C is the optimal temperature for some fungus, especially Aspergillus species [ 23 , 24 ]. However, the optimal production of crude cellulase was determined at 40 °C [ 25 ].…”

USE OF COMPOSITE AGRO-SUBSTRATES FOR AMYLOGLUCOSIDASE SYNTHESIS AND CHARACTERIZATION BY Aspergillus niger OTF AND Aspergillus flavus CLOR1 USING SOLID STATE FERMENTATION

“…Fermented substrate composition (0.5 g) was added to 15 ml acetate buffer solution (pH 5) agitated and mixed thoroughly. The suspension was filtered out and then centrifuged at 4000 rpm (Gupta et al, 2008). This solid-free supernatant was used as crude enzyme source for assaying amyloglucosidase activity.…”

“…The reaction was stopped by the addition 0.5 ml dinitrosalicylic acid reagent and heated for 5 min. Absorbance was recorded at 540 nm (Gupta et al, 2008). The enzyme was assayed and amyloglucosidase activity was recorded and calculated as follows:…”

Qualitative estimation of amylase enzyme activity of fungal species isolated from iron ore mined overburden soil

“…The distribution of these organisms is influenced by the abundance and nature of the organic content of the soil, as well as by other soil texture (Waksman 1944). Fungi are known agents of decomposition of organic matter, by producing extracellular enzymes (Reese & Levinson 1952, Lynd et al 2002, Chi et al 2007, Gupta et al 2008. Due to wide diversity, fungi have been recognized as a source of the new enzyme with useful and /or novel characteristics.…”