Production and Characterization of β-glucosidase Obtained by the Solid-State Cultivation of the Thermophilic Fungus Thermomucor indicae-seudaticae N31

Pereira, Joelma; Leite, Rodrigo Simões Ribeiro; Prado, Heloiza Ferreira Alves do; Bocchini, Daniela Alonso; Gomes, Eleni; Silva, Roberto da

doi:10.1007/s12010-014-1332-1

Cited by 20 publications

(9 citation statements)

References 26 publications

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“…Therefore, the first experiment in the present study was aimed at the verification of the co-production of GLA and β-carotene during SSF. Several common cereal substrates, which are well known as a good matrix for SSF and are easily accessible, were selected and screened for suitability of using them in SSF (Table 1) (Bogar et al 2003;Čertík et al 2013;de Cassia Pereira et al 2015;El-Naggar et al 2009). In general, owing to their composition, cereals offer all the components necessary for fungal proliferation.…”

Section: Resultsmentioning

confidence: 99%

Production of high-value bioproducts enriched with γ-linolenic acid and β-carotene by filamentous fungi Umbelopsis isabellina using solid-state fermentations

et al. 2020

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Solid-state fermentation is a useful tool for utilizing different plant-based materials as cultivation substrates in order to produce potentially high-value fermented bioproducts. The aim of the present study was to successfully prepare various types of such bioproducts, using a zygomycetous strain Umbelopsis isabellina CCF2412. Various legume and cereal substrates were utilized effectively, while a few of them were obtained from agricultural waste, which is particularly advantageous from ecological and economic point of view. A common feature of the produced fermented materials was the increased content of different polyunsaturated fatty acids and carotenoid pigments in these bioproducts. Subsequent to the optimization of the solid-state fermentation process using cornmeal as the cultivation substrate, bioproducts enriched with γ-linolenic acid (11.45 mg γ-linolenic acid per gram of bioproduct), β-carotene (50.90 μg β-carotene per gram of bioproduct), and various microbial sterols were obtained. Appropriate n-6/n-3 acid ratio and enrichment of other microbial substances, such as the pigments and sterols mentioned above, in the fermented bioproducts widens the applicability of these bioproducts in different industries. The fermented cereal bioproducts produced in the present study from fermented wheat bran substrate were used for evaluating their application as feed for broiler chicken, and satisfactory results were obtained. Therefore, the present study creates novel opportunities for improving the quality of fermented bioproducts obtained during solid-state fermentation processes, especially for application in the feed industry.

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Section: Resultsmentioning

confidence: 99%

Production of high-value bioproducts enriched with γ-linolenic acid and β-carotene by filamentous fungi Umbelopsis isabellina using solid-state fermentations

et al. 2020

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“…[20,30,32] Interestingly, Thermomucor, which has been found in Daqu in only a few studies, can secrete large amounts of various hydrolyzing enzymes, including protease, glucosidase, glucoamylase, lipase, and pectinase, which can decompose macromolecules. [28,[33][34][35][36][37][38][39] Thermomucor is therefore expected to be an important contributor to the saccharification and liquefication activities and to cause small metabolites and volatile compounds to be produced during Baijiu production.…”

Section: Prokaryote Community Structure and Diversitymentioning

confidence: 99%

Comprehensive analysis of different grades of roasted-sesame-like flavored Daqu

Fan

Chen

et al. 2019

International Journal of Food Properties

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We identified physicochemical, volatile compound, and microbial community differences between three grades of roasted-sesame-like flavored Daqu. The Daqu grades had different physicochemical characteristics. The concentrations and proportions of various esters reflected sensory evaluations of the Daqu classes but no rule linking the flavor compound types and concentrations to Daqu grade was identified. The prokaryotic microorganism communities were similar in premium-and first-grade Daqu but very different in general-grade Daqu. The eukaryotic microorganism communities in premium-and general-grade Daqu were similar, but the genus abundances were more similar in the premium-and first-grade Daqu samples than in premium-and general-grade Daqu. ARTICLE HISTORY

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“…The microbial suspension was obtained by adding 50 mL of nutrient solution containing 0.1% ammonium sulfate, 0.1% magnesium sulfate heptahydrate, and 0.1% ammonium nitrate (m/v), followed by gentle scraping of the medium surface. Inoculation of the microorganism was performed by transferring 5 mL of the microbial suspension to the flasks containing the agro-industrial residues (PEREIRA et al, 2015).…”

Section: Inoculummentioning

confidence: 99%

“…The reducing sugars released were quantified using the DNS method (MILLER, 1959). The activities of β-glucosidase and β-xylosidase were measured with the respective synthetic substrates, including 4 mM p-NP-β-Dglucopyranoside and 4 mM p-NP-β-Dxylopyranoside (Sigma) as described by Pereira et al (2015). All assays were performed at 50°C in 0.1 M sodium acetate buffer, pH 5.0.…”

Section: Determination Of Enzymatic Activitiesmentioning

confidence: 99%

Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries

Costa¹,

Scalabrini²,

Silvestre³

et al. 2016

Biosci. J.

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ABSTRACT:Xylanases are useful in several industrial segments, including pulp and paper bleaching, animal feed, and bread-making processes. However, the industrial use of these enzymes is closely related to its production cost and its catalytic properties. The process of solid state fermentation enables the use of agro-industrial residues as substrates for microbial cultivation and enzymes production, reducing costs. In the present study, different cultivation parameters were evaluated for the xylanase production by the thermophilic fungus Thermoascus aurantiacus, by solid state fermentation, using agro-industrial residues as substrates. High production of xylanase (1701.9 U g -1 of dry substrate) was obtained using wheat bran containing 65% of initial moisture, at 120 h of cultivation, and 45°C. The xylanase showed optimal activity at pH 5.0 and 75°C; its stability was maintained at pH 3.0-11.0. The enzyme retained its catalytic potential after 1 h, at 75°C. The enzymatic extract produced under optimized conditions showed reduced activities of endoglucanase and FPase. Our results, including the xylanase production by T. aurantiacus in low-cost cultivation medium, high structural stability of the enzyme, and reduced cellulolytic activity, encourage the application of this enzymatic extract in pulp and paper bleaching processes.

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Production and Characterization of β-glucosidase Obtained by the Solid-State Cultivation of the Thermophilic Fungus Thermomucor indicae-seudaticae N31

Cited by 20 publications

References 26 publications

Production of high-value bioproducts enriched with γ-linolenic acid and β-carotene by filamentous fungi Umbelopsis isabellina using solid-state fermentations

Production of high-value bioproducts enriched with γ-linolenic acid and β-carotene by filamentous fungi Umbelopsis isabellina using solid-state fermentations

Comprehensive analysis of different grades of roasted-sesame-like flavored Daqu

Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries

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