Production and composition of Pleurotus ostreatus cultivated on Lithovit®-Amino25 supplemented spent substrate

Abstract

Supplementation of the spent oyster substrate enhances its nutritional properties to produce a new mushroom cropping cycle. The study investigated the potential of a nano-fertilizer (Lithovit®-Amino25) with an admixture of 25% l-amino acids on Pleurotus ostreatus production, proteins, and amino acid contents. The product applied at spawning (t1), after the first harvest (t2), and at both timings (t3), in two doses: 3 g/kg (C1) or 5 g/kg (C2). Compared with control (C0t0), the first harvest was earlier by 2.3–3.3 days in C1t1 and C2t1. The biological yield of the second harvest was improved by 28.0% in C2t2. Superior results were in C1t3 where the number of crops increased to four, biological efficiency was optimized (117.3%) at the third harvest, and biological and economic yields increased by 36.7% and 36.4%, respectively. Lignin was the most degraded in C1t3, while residual cellulose, hemicellulose, neutral detergent fiber, and acid detergent fiber were higher in all treated substrates than in control. In C2t1, mushrooms were the richest in proteins, while in C1t1, they were the richest in the essential amino acids threonine, valine, isoleucine, leucine, and histidine. Lithovit®-Amino25 has a high potential for use in P. ostreatus production.

Combined effect of olive pruning residues and spent coffee grounds on Pleurotus ostreatus production, composition, and nutritional value

“…In global commercial production, the mushroom is grown on wheat straw (WS) substrate [11], however, some constraints related to the high cost of straw may arise in a number of countries where wheat straw is scarce, such as in Lebanon. Successful implementation of agro-industrial wastes in mushroom production has been delineated in a number of studies [12][13][14][15][16]. Particularly, the study conducted by Abou Fayssal et al [17] succeeded in reducing the dangerous hazard left over of olive pruning residues (OLPR) represented by open-field burning and fire risks.…”

Spent coffee grounds influence on Pleurotus ostreatus production, composition, fatty acid profile, and lignocellulose biodegradation capacity

“…The expensive importation and acquisition of commercial substrate had previously led to the use of locally available agro-industrial wastes (Abou Fayssal et al, 2020;Naim et al, 2020aNaim et al, , 2020bSassine et al, 2007;El Sebaaly et al, 2018a, 2018c, 2018b in mushroom production. At industrial scale, P. ostreatus is cultivated on cereal straw, mainly wheat straw (Labuschagne et al, 2000), which is becoming less available in many world regions causing constraints for oyster production (Picornell-Buendía et al, 2016).…”

Nano urea effects on Pleurotus ostreatus nutritional value depending on the dose and timing of application

“…Among all treatments, essential amino acids were the highest in mushrooms of the treatment C 1 t 1 , where threonine, valine, isoleucine, leucine, phenylalanine, histidine, lysine, and methionine contents increased by 0.31, 0.10, 0.05, 0.21, 0.18, 2.05, 0.27, and 0.09%, respectively compared to control. Another nano-supplement, rich in amino acids (Lithovit-Amino25) improved most of the essential amino acids in P. ostreatus mushroom when applied at spawning 65 . On the other hand, at the second timing of application (after the first harvest), both product doses have caused significant reduction in essential amino acids contents in mushrooms, compared to control, with few exceptions (respective increase of histidine content by 1.11 and 1.37% in C 1 t 2 and C 2 t 2 , and of methionine content by 0.02% in C 1 t 2 ).…”

“…Among all treatments, essential amino acids were the highest in mushrooms of the treatment C 1 t 1 , where threonine, valine, isoleucine, leucine, phenylalanine, histidine, lysine, and methionine contents increased by 0.31, 0.10, 0.05, 0.21, 0.18, 2.05, 0.27, and 0.09%, respectively compared to control. Another nano-supplement, rich in amino acids (Lithovit-Amino25) improved most of the essential amino acids in P. ostreatus mushroom when applied at spawning 65 .…”