1987
DOI: 10.1016/0378-1119(87)90033-3
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Production and detection of coliphage T4 endonuclease V polyclonal and monoclonal antibodies using staphylococcal protein-A hybrid proteins

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Cited by 14 publications
(3 citation statements)
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“…One was T4 PDG (pyrimidine dimer glycosylase) endonuclease V (T4-endoV), prepared in our laboratory from an E. coli strain carrying a plasmid encoding the phage T4 DEN V gene, 26 and the other formamidopyrimidine [fapy]-DNA glycosylase (Fpg) (from New England BioLabs, Inc). 27 For the digestion assay, 100-200 ng of plasmid DNA was diluted in the corresponding enzyme buffer.…”
Section: Dna Damage Detectionmentioning
confidence: 99%
“…One was T4 PDG (pyrimidine dimer glycosylase) endonuclease V (T4-endoV), prepared in our laboratory from an E. coli strain carrying a plasmid encoding the phage T4 DEN V gene, 26 and the other formamidopyrimidine [fapy]-DNA glycosylase (Fpg) (from New England BioLabs, Inc). 27 For the digestion assay, 100-200 ng of plasmid DNA was diluted in the corresponding enzyme buffer.…”
Section: Dna Damage Detectionmentioning
confidence: 99%
“…By this approach, we hoped to produce enough protein for effective immunizations and subsequent screening of hybridomas. Similar protocols have been used by others to generate antibodies to proteins that are difficult to purify from cells where they are expressed naturally (22)(23)(24). We report the production of 4 mabs to human perforin.…”
mentioning
confidence: 90%
“…Endonuclease V was detected on nitrocellulose filters essentially as described (21) except that the mouse monoclonal antibodies (2-F4 and 1-C3; ref. 21) specific for endonuclease V were detected by incubation with rabbit anti-mouse IgG (heavy and light chain) antibody (Cooper Biomedical), followed by incubation with 125I-labeled protein A (30-70,aCi/jug; ICN; 1 pCi = 37 kBq).…”
mentioning
confidence: 99%