Production and estimation of alkaline protease by immobilized Bacillus licheniformis isolated from poultry farm soil of 24 Parganas and its reusability

Abstract

Microbial alkaline protease has become an important industrial and commercial biotech product in the recent years and exerts major applications in food, textile, detergent, and pharmaceutical industries. By immobilization of microbes in different entrapment matrices, the enzyme produced can be more stable, pure, continuous, and can be reused which in turn modulates the enzyme production in an economical manner. There have been reports in support of calcium alginate and corn cab as excellent matrices for immobilization of Bacillus subtilis and Bacillus licheniformis, respectively. This study has been carried out using calcium alginate, κ-carrageenan, agar-agar, polyacrylamide gel, and gelatin which emphasizes not only on enzyme activity of immobilized whole cells by different entrapment matrices but also on their efficiency with respect to their reusability as first attempt. Gelatin was found to be the best matrix among all with highest enzyme activity (517 U/ml) at 24 h incubation point and also showed efficiency when reused.

Characterization of thermostable alkaline proteases from Bacillus infantis SKS1 isolated from garden soil

“…Alkaline protease producing bacteria are ubiquitously found in natural [ 13 – 16 ] and, anthropogenic man-made environment [ 17 ]. As compared to plants and animals as a source of proteases [ 6 , 7 ], it is more propitious to use microbes because of their enormous diversity, expeditious growth, requirement for limited space during cultivation and easy genetic manipulation [ 18 , 19 ]. Bacillus is one of the most vital genera that have been used for alkaline proteases production because of their chemoorganotrophic characteristics, tremendous growth rates, secretion of extracellular enzyme into media and are safe to handle [ 2 , 20 ].…”

Purification and biochemical characterization of a novel thermostable serine alkaline protease from Aeribacillus pallidus C10: a potential additive for detergents

“…Scientists are quite intensively interested in alkaline proteases that are produced by microorganisms and are quite important in terms of biotechnology 9 . The reason for this is that the enyzmes originating from microorganisms have high-catalytic activities, that they do not cause any undesired by-product, that their growth conditions can be easily optimized, that they are quite inexpensive, and that they can be produced in quite large amounts with high purity and at a single production process 10 , 11 . Apart from the fact that alkaline proteases are produced by a large microorganism group in which fungi and bacteria are also included, a great majority of commercial alkaline proteases in particular are obtained from the bacteria of Bacillus sp.…”

Immobilization of Bacilli in Various Matrices to Enhance the Utilization of Keratinase and its Comparison

“…Immobilized beads displayed high level of heat stability and increased tolerance towards acidic pH compared with the free keratinase (R. Manju, 2013). Immobilization of alkaline protease producing Bacillus licheniformis in calcium alginate, κ-carrageenan, agar-agar, polyacrylamide gel, and gelatin was successful and reusable (Shamba Chatterjee, 2015). The results of our study clearly indicate that with immobilization of whole cell has application potential and can greatly enhance the recycling potential of the enzyme for 3 batches spanning for more 21 days (Fig.…”