Production and extraction of carotenoids produced by microorganisms

Mussagy, Cassamo U.; Winterburn, James; Santos-Ebinuma, Valéria de Carvalho; Pereira, Jorge Fernando Brandão

doi:10.1007/s00253-018-9557-5

Cited by 165 publications

(103 citation statements)

References 135 publications

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“…All the reports that have studied the effect of metal on carotenoid production confirmed its stimulatory role on carotenoid production, explaining this by hypothesising a possible activation or inhibition mechanism of selected metal ions on specific carotenogenic enzymes, in particular, on a specific desaturase involved in carotenoid biosynthesis [14]. Elsewhere, it was noted that the determination of physiological and biochemical changes of Rhodotorula mucilaginosa AN5 after progressive copper treatment stated a significant increase in the antioxidative reagents content and enzymes activity, which quenched the active oxygen species to 3 concentrations on growth, and lipid and carotenoid production by Rhodotorula glutinis. Yeast cells were grown in the shaken flask using the optimised medium as a control with C/N equal to 146 (using ammonium sulfate as a sole nitrogen source) and C/S equal to 120, and incubated at180 rpm and 28 • C for six days: (a) dry cell weight (DCW) (g/L), (b) total lipid (TL (g/L)) and lipid relative productivity (LRP = (g lipid /g DCW ) × 100), and (c) total pigment (TP mg/L) and carotenoid relative productivity (Car-RP mg pigment /100 g DCW ).…”

Section: Impact Of Aluminium Sulfate On Lipogenesis and Carotenoid Prmentioning

confidence: 82%

“…This is the first report on the role of Al 2 (SO 4 ) 3 for enhancing torulene production under lipogenesis condition, which could be used as a potential tool for torulene production. microorganisms are algae, bacteria, filamentous fungi, and yeasts, such as Streptomyces chrestomyceticus, Blakeslea trispora, Phycomyces blakesleeanus, Flavobacterium sp., Phaffia sp., and Rhodotorula sp., have been described as highly carotenoid-producing microorganisms [3,4].Rhodotorula glutinis (R. glutinis) is capable of synthesizing numerous valuable compounds with a wide industrial usage. This yeast is a red oleaginous yeast that produces a significant amount of both lipids and carotenoids.…”

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confidence: 99%

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Lipid and Carotenoid Production by Rhodotorula glutinis with a Combined Cultivation Mode of Nitrogen, Sulfur, and Aluminium Stress

et al. 2019

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Torulene is a promising pink pigment, produced only by yeasts and fungi, and its production is still in a developing stage due to the low production rate. Accordingly, this study focuses on maximizing torulene production by Rhodotorula glutinis using shaken flask fermentation. The effect of different nitrogen sources, and C/N and C/S ratios on lipid and carotenoid production by R. glutinis was studied using 60 g/L glucose. The largest cells filled with golden fluorescence lipid bodies were observed using fluorescence microscopy when peptone was used as a nitrogen source. The highest total pigment (0.947 mg/L) and carotenoid relative productivity (Car-RP) (89.04 µg/g) were obtained at C/N 146 and C/S 120, and with ammonium sulfate as a nitrogen source, with 62% torulene domination using High Performance Liquid Chromatography (HPLC) for identification. Under a high C/N ratio, regardless of the C/S ratio, the carotenoid synthesis rate decreased after three days while the lipid synthesis rate kept increasing to the sixth day. Interestingly, after adding 0.7 mM Al2(SO4)3 to the optimized medium, the total pigment and Car-RP (2.2 mg/L and 212.9 µg/g) sharply increased, producing around 2.16 mg/L torulene (98%) with around 50% decrease in lipid yield. This is the first report on the role of Al2(SO4)3 for enhancing torulene production under lipogenesis condition, which could be used as a potential tool for torulene production.

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Section: Impact Of Aluminium Sulfate On Lipogenesis and Carotenoid Prmentioning

confidence: 82%

mentioning

confidence: 99%

Lipid and Carotenoid Production by Rhodotorula glutinis with a Combined Cultivation Mode of Nitrogen, Sulfur, and Aluminium Stress

et al. 2019

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“…5 ). Carotenoids are yellow, orange, and red organic pigments that are widely distributed in diverse lineages of organisms on Earth ( Fraser and Bramley 2004 ; Mussagy et al. 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomics Underlines Multiple Roles of Profftella, an Obligate Symbiont of Psyllids: Providing Toxins, Vitamins, and Carotenoids

Nakabachi

Piel

Malenovský

et al. 2020

Genome Biology and Evolution

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The Asian citrus psyllid Diaphorina citri (Insecta: Hemiptera: Psylloidea), a serious pest of citrus species worldwide, harbors vertically transmitted intracellular mutualists, Candidatus Profftella armatura (Profftella_DC, Gammaproteobacteria: Burkholderiales) and Candidatus Carsonella ruddii (Carsonella_DC, Gammaproteobacteria: Oceanospirillales). Whereas Carsonella_DC is a typical nutritional symbiont, Profftella_DC is a unique defensive symbiont with organelle-like features including intracellular localization within the host, perfect infection in host populations, vertical transmission over evolutionary time, and drastic genome reduction down to much less than 1 Mb. Large parts of the 460 kb genome of Profftella_DC are devoted to genes for synthesizing a polyketide toxin; diaphorin. To better understand the evolution of this unusual symbiont, the present study analyzed the genome of Profftella_Dco, a sister lineage to Profftella_DC, using Diaphorina cf. continua, a host psyllid congeneric with D. citri. The genome of co-residing Carsonella (Carsonella_Dco) was also analyzed. The analysis revealed nearly perfect synteny conservation in these genomes with their counterparts from D. citri. The substitution rate analysis further demonstrated genomic stability of Profftella which is comparable to that of Carsonella. Profftella_Dco and Profftella_DC shared all genes for the biosynthesis of diaphorin, hemolysin, riboflavin, biotin, and carotenoids, underlining multiple roles of Profftella, which may contribute to stabilizing symbiotic relationships with the host. However, acyl carrier proteins (ACPs) were extensively amplified in polyketide synthases DipP and DipT for diaphorin synthesis in Profftella_Dco. This level of ACP augmentation, unprecedented in modular polyketide synthases of any known organism, is not thought to influence the polyketide structure but may improve the synthesis efficiency.

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“…SCF temperature and pressure are above the critical point, thereby presenting properties that explain its greater ability to diffuse into the matrix than conventional organic solvents. Many studies deal with SC‐CO 2 extraction of carotenoids (Bustamante, Roberts, Aravena, & Del Valle, ; Hosseini, Tavakoli, & Sarrafzadeh, ; Machmudah, Shotipruk, Goto, Sasaki, & Hirose, ; Macías‐Sanchez, Serrano, Rodríguez, & de la Ossa, ; Mussagy, Winterburn, Santos‐Ebinuma, & Pereira, ) and lipids (Mendes, Reis, & Palavra, ; Sajilata, Singhal, & Kamat, ) from microalgae and yeasts (Hasan, Azhar, Nangia, Bhatt, & Panda, ; Lim, Lee, Lee, Haam, & Kim, ; Wang, Chen, Rakesh, Qin, & Lv, ). Co‐solvents that enhance the solubilizing power of SC‐CO 2 such as ethanol or vegetable oils are occasionally needed to increase the extraction yield (Lim et al., ).…”

Section: Obtaining Highly Valuable Compounds From Microbial Biomassmentioning

confidence: 99%

Pulsed electric field‐assisted extraction of valuable compounds from microorganisms

Martínez

Delso

Álvarez

et al. 2020

Comp Rev Food Sci Food Safe

137

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Microorganisms (bacteria, yeast, and microalgae) are a promising resource for products of high value such as nutrients, pigments, and enzymes. The majority of these compounds of interest remain inside the cell, thus making it necessary to extract and purify them before use. This review presents the challenges and opportunities in the production of these compounds, the microbial structure and the location of target compounds in the cells, the different procedures proposed for improving extraction of these compounds, and pulsed electric field (PEF)‐assisted extraction as alternative to these procedures. PEF is a nonthermal technology that produces a precise action on the cytoplasmic membrane improving the selective release of intracellular compounds while avoiding undesirable consequences of heating on the characteristics and purity of the extracts. PEF pretreatment with low energetic requirements allows for high extraction yields. However, PEF parameters should be tailored to each microbial cell, according to their structure, size, and other factors affecting efficiency. Furthermore, the recent discovery of the triggering effect of enzymatic activity during cell incubation after electroporation opens up the possibility of new implementations of PEF for the recovery of compounds that are bounded or assembled in structures. Similarly, PEF parameters and suspension storage conditions need to be optimized to reach the desired effect. PEF can be applied in continuous flow and is adaptable to industrial equipment, making it feasible for scale‐up to large processing capacities.

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Production and extraction of carotenoids produced by microorganisms

Cited by 165 publications

References 135 publications

Lipid and Carotenoid Production by Rhodotorula glutinis with a Combined Cultivation Mode of Nitrogen, Sulfur, and Aluminium Stress

Lipid and Carotenoid Production by Rhodotorula glutinis with a Combined Cultivation Mode of Nitrogen, Sulfur, and Aluminium Stress

Comparative Genomics Underlines Multiple Roles of Profftella, an Obligate Symbiont of Psyllids: Providing Toxins, Vitamins, and Carotenoids

Pulsed electric field‐assisted extraction of valuable compounds from microorganisms

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