Production and fingerprinting of virus-free clones in a reflowering globe artichoke

Acquadro, Alberto; Papanice, M.A.; Lanteri, Sergio; Bottalico, Giovanna; Portis, Ezio; Campanale, A.; Finetti-Sialer, M.; Mascia, Tiziana; Sumerano, P.; Gallitelli, D.

doi:10.1007/s11240-009-9654-3

Cited by 33 publications

(24 citation statements)

References 36 publications

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“…Indeed, a set of thirty of the most promising genotypes has been vegetatively propagated by isolating basal growing shoots, and are currently under evaluation in different environments with the goal of identifying the most valuable for future marketing. Micropropagation is a further option, as protocols for meristem culture have been well established for the species, also starting from meristem tips, with the goal to obtain virus free plants for the production of sanitary controlled propagative material (Acquadro et al 2010;Barba et al 2004;Papanice et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Morphology and SSR fingerprinting of newly developed Cynara cardunculus genotypes exploitable as ornamentals

et al. 2011

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The species Cynara cardunculus includes the globe artichoke (var. scolymus), the cultivated cardoon (var. altilis) and the wild cardoon (var. sylvestris). The three taxa are sexually compatible and originate fertile F 1 progenies, which, given the high heterozygosity of the species, are highly segregating. We report the characterization of two F 1 populations, one bred from a cross between globe artichoke and cultivated cardoon, and the other between globe artichoke and wild cardoon. Both populations featured a wide array of phenotypes in relation to several traits, and some of the newly developed genotypes are of interest for the ornamental market. The two populations were genotyped at 50 microsatellite (SSR) loci: in the globe artichoke 9 wild cardoon and globe artichoke 9 cultivated cardoon progenies 116 and 97 alleles were respectively detected. SSR pattern scores were used to produce an UPGMA dendrogram and a PCoA plot. A set of nine SSR loci, evenly dispersed across the genome, was shown to be sufficient to unambiguously identify each segregant. The molecular fingerprinting is useful for establishing the true to type correspondence of propagative materials in nurseries and ensures the effective correspondence between the real and the declared identity of a clone.

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Section: Discussionmentioning

confidence: 99%

Morphology and SSR fingerprinting of newly developed Cynara cardunculus genotypes exploitable as ornamentals

et al. 2011

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“…Molecular markers such as AFLP (Lanteri et al 2004a;Pagnotta et al 2004;Portis et al 2005;Mauro et al 2009;Acquadro et al 2010), RAPD (Lanteri et al 2001;Sonnante et al 2002), ISSR (Crinò et al 2008;Lo Bianco et al 2011), and SSR (Acquadro et al 2005;Sonnante et al 2008;Mauro et al 2009) have been developed and are already applied to globe artichoke. ISSR, RAPD and AFLP markers have been used for assessments of genetic diversity in the different globe artichoke types i.e.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Italian spring globe artichoke germplasm: morphological and molecular profiles

et al. 2012

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Globe artichoke is native to the Mediterranean basin with Italy being the leading world producer. Italian autochthonous germplasm is at risk of genetic erosion due to the large cultivation of only a few varietal types, such as the micropropagated clone C3, and to the introduction of new seed-propagated genetic materials. The aim of our study is to assess the genetic variability existing in nine Italian globe artichoke landraces of spring type, using morphological descriptors and DNA markers (Amplified Fragment Lenght Polymorphism and Inter-Simple Sequence Repeats). A significant genetic differentiation among genotypes was found for many traits. Although some genetic variability found within each genotype was probably due to their multiclonal origin, most of them were genetically welldefined. Morphological and molecular profiles give important information to complete the genotype characterization and proceed with the selection of clones to be propagated and to develop lines and/or varieties. The present paper underlines the differences existing in the analyzed germplasm both within and among landraces and the genetic relations among different material, which is an important starting point for future plant breeding programs.

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“…Molecular markers can be used to identify populations and landraces, clustering populations and genotypes, as well as genotype fingerprinting. In the last decade several molecular markers have been utilized in Cynara cardunculus populations, such as AFLP (Lanteri et al 2004a;Pagnotta et al 2004;Portis et al 2005;Mauro et al 2009;Acquadro et al 2010), RAPD (Lanteri et al 2001;Sonnante et al 2002), ISSR (Crinò et al 2008;Lo Bianco et al 2011) and SSR (Acquadro et al 2005;Sonnante et al 2008;Mauro et al 2009). The molecular markers were used mainly for variety identification and genetic diversity and relatedness investigations (Tivang et al 1996;Sonnante et al 2002Sonnante et al , 2008Crinò et al 2008), plant breeding as selection tools and to characterize parental lines in F1 hybrid constitution (Lo Bianco et al 2011), as well as to identify the most probable progenitors of a given hybrid (Messmer et al 2002).…”

Section: Molecular Assessmentmentioning

confidence: 99%

Genetic Diversity Assessment in European Cynara Collections

Pagnotta

Noorani²

2013

Genomics of Plant Genetic Resources

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Production and fingerprinting of virus-free clones in a reflowering globe artichoke

Cited by 33 publications

References 36 publications

Morphology and SSR fingerprinting of newly developed Cynara cardunculus genotypes exploitable as ornamentals

Morphology and SSR fingerprinting of newly developed Cynara cardunculus genotypes exploitable as ornamentals

Characterization of Italian spring globe artichoke germplasm: morphological and molecular profiles

Genetic Diversity Assessment in European Cynara Collections

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