Production and function of cytokines in natural and acquired immunity to Candida albicans infection.

Ashman, R. B.; Papadimitriou, John

doi:10.1128/mmbr.59.4.646-672.1995

Cited by 95 publications

(46 citation statements)

References 244 publications

(289 reference statements)

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“…Similarly, the synthesis of anti-K and anti-L oligomannoside antibodies has been shown to be di¡erently regulated in patients colonized and infected by C. albicans [23]. Together, these data emphasize the importance of a comprehensive approach involving structure/immunoreactivity of C. albicans mannan derived oligomannoside for rationalization of both pathophysiological approaches [24,25] and diagnostic procedures [2].…”

Section: Discussionmentioning

confidence: 91%

Nature of Candida albicans-derived carbohydrate antigen recognized by a monoclonal antibody in patient sera and distribution over Candida species

Jacquinot

1998

FEMS Microbiology Letters

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The minimal epitope of an anti-Candida albicans mannan monoclonal antibody (MAb) EB-CA1, used to detect mannanemia in patient sera, was determined. MAb EB-CA1 exhibited reactivity with oligomannosides released from the mannan acid stable domain, converted into neoglycolipids (NGLs) and coated onto ELISA plates. Reactivity occurred with mannopentaose and higher oligomers, whereas mannotriose and mannotetraose were unreactive. MAb EB-CA1 binding to mannan acid stable mannopentaose NGL displayed a dose dependent and saturable specific reactivity curve whereas there was a complete absence of binding, even at high concentrations, with NGLs constructed from the L-1,2-linked mannopentaose derived from the mannan acid labile fraction. MAb EB-CA1 binding to acid stable mannopentaose NGL was inhibited by the homologous oligomannoside but not by mannotriose and mannotetraose. NMR analysis showed that mannotriose and mannotetraose contained exclusively K-1,2-linked D-mannopyranose units and that mannopentaose was a mixture of a mannopentaose K-1,2-linked and an isomer in which the fifth mannose was K-1,6-linked to the reducing unit of manno-K-1,2 tetraose. Western blot analysis has shown that MAb EB-CA1 epitope was expressed on a wide range of C. albicans manno-glycoconjugate as well as on manno-glycoconjugates of other pathogenic species of the genus Candida, viz. C. tropicalis, C. glabrata, C. parapsilosis and C. krusei. z

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Section: Discussionmentioning

confidence: 91%

Nature of Candida albicans-derived carbohydrate antigen recognized by a monoclonal antibody in patient sera and distribution over Candida species

Jacquinot

1998

FEMS Microbiology Letters

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“…Because macrophages play a crucial role in the defence against C. albicans (Ashman and Papadimitriou, 1995), we further investigated synergistic signalling between dectin-1 and TLR2 or TLR4 in human monocyte-derived macrophages. Similar to the effects in monocytes, the combinations of curdlan with Pam3Cys or LPS exerted robust synergistic effects on cytokine production in human primary macrophages ( Fig.…”

Section: Synergy Between Dectin-1 and Tlrs In Monocyte-derived Macropmentioning

confidence: 99%

Dectin-1 synergizes with TLR2 and TLR4 for cytokine production in human primary monocytes and macrophages

Ferwerda¹,

Meyer‐Wentrup²,

Kullberg³

et al. 2008

Cellular Microbiology

306

241

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SummaryThe b-glucan receptor dectin-1 and Toll-like receptors TLR2 and TLR4 are the main receptors for recognition of Candida albicans by the innate immune system. It has been reported that dectin-1 amplifies TLR2-dependent induction of cytokines in mouse models. In the present study we hypothesized that dectin-1 has potent synergistic effects with both TLR2 and TLR4 in human PBMCs and macrophages. Human PBMCs and monocyte-derived macrophages were stimulated with curdlan, a linear b-1,3-glucan-polymer derived from Alcaligenes faecalis with specific ligand affinity for dectin-1, in combination with the synthetic TLR2 ligand Pam3Cys and the ultrapure TLR4 ligand LPS. TNF-a and IL-10 production was measured in the supernatants with ELISA. Curdlan is a specific dectin-1 ligand without TLR2-or TLR4-stimulating properties. Human primary monocytes and macrophages express dectin-1 on the cell membrane. Stimulation of human PBMCs with curdlan in combination with Pam3Cys or LPS leads to synergistic increase in TNF-a production that was inhibited by GE2, a neutralizing dectin-1 antibody. Dectin-1-dependent synergy between curdlan and TLR agonists was also apparent in human monocyte-derived macrophages. Conclusively, dectin-1 synergizes with both TLR2 and TLR4 pathways for the production of TNF-a in human primary PBMCs and in monocyte-derived macrophages.

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“…A variety of mammalian species have been employed to elucidate the role of the immune system in combating fungal infections [1^6] and have contributed to our understanding of the interactions between pathogenic fungi and host tissue in cases of human disease [7,8]. In addition, animal models have proved invaluable in establishing the pathogenicity of various fungi through in vivo tests [9,10].…”

Section: Introductionmentioning

confidence: 99%

Development of an insect model for the in vivo pathogenicity testing of yeasts

Cotter

2000

FEMS Immunology and Medical Microbiology

111

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Conventional in vivo assays to determine the relative pathogenicity of yeast isolates rely upon the use of a range of mammalian species. The purpose of the work presented here was to investigate the possibility of using an insect (Galleria mellonella) as a model system for in vivo pathogenicity testing. The haemolymph of G. mellonella larvae was inoculated with PBS containing different concentrations of stationary phase yeasts of the genus Candida by injection at the last pro-leg. Larvae were incubated at 30 degrees C and monitored over 72 hours. Results indicate that G. mellonella can be killed by the pathogenic yeast Candida albicans and by a range of other Candida species but not to a significant extent by the yeast Saccharomyces cerevisiae. The kill kinetics for larvae inoculated with clinical and laboratory isolates of C. albicans indicate the former class of isolates to be more pathogenic. Differences in the relative pathogenicity of a range of Candida species may be distinguished using G. mellonella as a model. This work indicates that G. mellonella may be employed to give results consistent with data previously obtained using mammals in conventional in vivo pathogenicity testing. Larvae of G. mellonella are inexpensive to culture, easy to manipulate and their use may reduce the need to employ mammals for routine in vivo pathogenicity testing with a concomitant reduction in mammalian suffering.

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Production and function of cytokines in natural and acquired immunity to Candida albicans infection.

Cited by 95 publications

References 244 publications

Nature of Candida albicans-derived carbohydrate antigen recognized by a monoclonal antibody in patient sera and distribution over Candida species

Nature of Candida albicans-derived carbohydrate antigen recognized by a monoclonal antibody in patient sera and distribution over Candida species

Dectin-1 synergizes with TLR2 and TLR4 for cytokine production in human primary monocytes and macrophages

Development of an insect model for the in vivo pathogenicity testing of yeasts

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