Production and Kinetics of Salmonella enterica serovar enteritidis in Vibrofermentor

Lipopolysaccharide (LPS) and flagellin are the major antigenic structures of Salmonella Enteritidis. Purified antigens are essential components for the diagnosis of and research on Salmonella Enteritidis. In this study, LPS were isolated and purified with modified hot phenol-water procedure in a yield of 6%. The flagellin isolation and purification were done by strong shaking with glass pearl beads with 3.9 mg/ml protein content. Purity was demonstrated on SDS-PAGE, ELISA and with colorimetric assay for both isolated antigens. The antigen-mediated ELISA results exhibited immunoreactive properties for both LPS and flagellar antigen specific for Salmonella Enteritidis O:9 and H:g,m, respectively. The findings demonstrated low reactivity with Mab O:9 for isolated LPS due to their monoepitopic specificity. However, isolated flagellin reactivity was high with Mab H:g,m related to their cross-reactivity potential between H:g and H:m. Additionally in this study.the results indicated that similar antigenic structures have alternative potential for antigenic isolation of Salmonella Enteritidis, such as Salmonella Gallinarum for LPS antigen and Salmonella Adeyo for flagellar antigen isolation.

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Isolation and purification of O and H antigens from Salmonella Enteritidis as diagnostic tool

Nalbantsoy

Karaboz

Ivanova

et al. 2010

Ann Microbiol

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Production of Monoclonal Antibodies in a Mouse Model via Lipopolysaccharide Conjugates with Synthetic Polymers Specific toSalmonellaEnteritidis O Antigen

Nalbantsoy

Karaboz²,

Gürhan³

2010

Foodborne Pathogens and Disease

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Monoclonal antibodies (Mabs) specific for lipopolysaccharide (LPS) of Salmonella Enteritidis were evaluated in a model of LPS conjugated synthetic polymer immunization of Balb/c mice by conventional hybridoma method. Nine hybridoma cell lines were determined as antibody positive against LPS. The clone 5A8 secreting the highest antibody was selected for further characterization. Evaluated results indicate that the synthetic polymer can be used as an effective adjuvant in immunization with LPS, because the 5A8 Mab were obtained using synthetic polymer as an adjuvant. 5A8 Mab was classified as IgG2a isotype by antibody capture enzyme-linked immunosorbent assay. The reactivity of the Mab against lipid A and different LPS of Salmonella were investigated using an indirect enzyme-linked immunosorbent assay. Mab presented a wide spectrum of reactivity, coupling with antigens against Salmonella. The hybridoma 5A8 determined in this study has a great potential to be used in the development of diagnostic, prophylactic, and therapeutic agents specific for Salmonella Enteritidis and Salmonella LPS.

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Production and Kinetics of Salmonella enterica serovar enteritidis in Vibrofermentor

Cited by 2 publications

References 8 publications

Isolation and purification of O and H antigens from Salmonella Enteritidis as diagnostic tool

Isolation and purification of O and H antigens from Salmonella Enteritidis as diagnostic tool

Production of Monoclonal Antibodies in a Mouse Model via Lipopolysaccharide Conjugates with Synthetic Polymers Specific toSalmonellaEnteritidis O Antigen

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