Production and Molecular Cytogenetic Identification of Wheat-Alien Hybrids and Introgression Lines

Molnár-Láng, Márta; Molnár, István; Szakács, Éva; Linc, Gabriella; Bedö, Z.

doi:10.1007/978-94-007-7572-5_11

Cited by 22 publications

(10 citation statements)

References 164 publications

(155 reference statements)

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“…It has been reported that some Ae. biuncialis accessions have good tolerance of heat and drought stress (Molnár et al, 2004; Dulai et al, 2014) and others are promising for resistance to rust diseases (Molnár-Láng et al, 2014; Olivera et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Unlocking the Genetic Diversity and Population Structure of a Wild Gene Source of Wheat, Aegilops biuncialis Vis., and Its Relationship With the Heading Time

et al. 2019

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Understanding the genetic diversity of Aegilops biuncialis, a valuable source of agronomical useful genes, may significantly facilitate the introgression breeding of wheat. The genetic diversity and population structure of 86 Ae. biuncialis genotypes were investigated by 32700 DArT markers with the simultaneous application of three statistical methods— neighbor-joining clustering, Principal Coordinate Analysis, and the Bayesian approach to classification. The collection of Ae. biuncialis accessions was divided into five groups that correlated well with their eco-geographic habitat: A (North Africa), B (mainly from Balkans), C (Kosovo and Near East), D (Turkey, Crimea, and Peloponnese), and E (Azerbaijan and the Levant region). The diversity between the Ae. biuncialis accessions for a phenological trait (heading time), which is of decisive importance in the adaptation of plants to different eco-geographical environments, was studied over 3 years. A comparison of the intraspecific variation in the heading time trait by means of analysis of variance and principal component analysis revealed four phenotypic categories showing association with the genetic structure and geographic distribution, except for minor differences. The detailed exploration of genetic and phenologic divergence provides an insight into the adaptation capacity of Ae. biuncialis, identifying promising genotypes that could be utilized for wheat improvement.

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Section: Discussionmentioning

confidence: 99%

Unlocking the Genetic Diversity and Population Structure of a Wild Gene Source of Wheat, Aegilops biuncialis Vis., and Its Relationship With the Heading Time

et al. 2019

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“…Common wheat ( Triticum aestivum L.) is 1 of the most important crops in the world. For higher yield and better resistance to diseases, many useful genes or factors have been introduced into wheat through chromosome translocation or substitution from many different relative genera ( Molnár-Láng et al, 2014 ; Feldman and Levy, 2015 ) such as Pm53, Yr40 , and Yr42 from Aegilops spp. ( Marais et al, 2009 ; Kuraparthy et al, 2009 ; Petersen et al, 2015 ), Pm40 and Yr50 from Thinopyrum spp.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cytogenetic Characterization of Novel Wheat-rye T1RS.1BL Translocation Lines with High Resistance to Diseases and Great Agronomic Traits

Ren

Tang

et al. 2017

Front. Plant Sci.

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Rye has been used worldwide as a source for the genetic improvement of wheat. In this study, two stable wheat-rye primary T1RS.1BL translocation lines were selected from the progeny of the crossing of the wheat cultivar Mianyang11-1 and a Chinese local rye variety, Weining. These two novel translocation lines were identified by molecular cytogenetic analysis. PCR results, multi-color fluorescence in situ hybridization (MC-FISH), and acid polyacrylamide gel electrophoresis (A-PAGE) indicated that both new translocation lines harbor a pair of T1RS.1BL translocation chromosomes, and have been named RT828-10 and RT828-11, respectively. The cytogenetic results also indicated that the pSc119.2 signals of 5AL were absent in both lines along with the pSc119.2 signals of 4AL of RT828-11. When inoculated with different stripe rust and powdery mildew isolates, both lines expressed high resistance to Puccinia striiformis f. sp. tritici and Blumeria graminis f. sp. tritici pathotypes, which are prevalent in China and are virulent on Yr9 and Pm8. The line RT828-11 also exhibited excellent agronomic traits in the field. The present study indicates that this rye variety may carry untapped variations that could potentially be used for wheat improvement.

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“…The development of new wheat cultivars with higher yield and good resistance to diseases is the eternal goal of breeders. The alien substitutions or translocations of chromosomes between wheat and its relative species have played an important role in wheat improvement [ 1 , 2 , 3 , 4 ]. Many alien genes have been transferred into bread wheat through chromosome translocations from its different relative genera, such as Secale cereale [ 5 , 6 ], Hordeum californicum [ 7 ], Leymus mollis [ 8 ], Agropyron elongatum [ 9 ], Haynaldia villosa [ 10 ], Thinopyrum [ 2 , 11 ], and Aegilops peregrina [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Cytogenetic Characterization of New Wheat-Rye 1R(1B) Substitution and Translocation Lines from a Chinese Secale cereal L. Aigan with Resistance to Stripe Rust

Ren

Tan

et al. 2016

PLoS ONE

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Secale cereale L. has been used worldwide as a source of genes for agronomic and resistance improvement. In this study, a stable wheat-rye substitution line and 3 primary 1RS.1BL translocation lines were selected from the progeny of the crossing of the Chinese local rye Aigan variety and wheat cultivar Mianyang11. The substitution and translocation lines were identified by molecular cytogenetic analysis. PCR results, fluorescence in situ hybridization and acid polyacrylamide gel electrophoresis indicated that there were a pair of 1R chromosomes in the substitution line which have been named RS1200-3, and a pair of 1RS.1BL translocation chromosomes in the other 3 translocation lines, which have been named RT1163-4, RT1217-1, and RT1249. When inoculated with stripe rust isolates, these 4 lines expressed high resistance to several Puccinia striiformis f. sp Tritici pathotypes that are virulent on Yr9. Moreover, the different response pattern of resistance among them suggested that the diversity of resistance genes for wheat stripe rust exists in the rye. These 4 lines also showed better agronomic performances than their wheat parent. The GS indices also showed the genetic diversity of the 1RS which derived from same rye variety. The present study indicates that rye cultivars may carry untapped variations that could potentially be used for wheat improvement.

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Production and Molecular Cytogenetic Identification of Wheat-Alien Hybrids and Introgression Lines

Cited by 22 publications

References 164 publications

Unlocking the Genetic Diversity and Population Structure of a Wild Gene Source of Wheat, Aegilops biuncialis Vis., and Its Relationship With the Heading Time

Unlocking the Genetic Diversity and Population Structure of a Wild Gene Source of Wheat, Aegilops biuncialis Vis., and Its Relationship With the Heading Time

Molecular Cytogenetic Characterization of Novel Wheat-rye T1RS.1BL Translocation Lines with High Resistance to Diseases and Great Agronomic Traits

Molecular Cytogenetic Characterization of New Wheat-Rye 1R(1B) Substitution and Translocation Lines from a Chinese Secale cereal L. Aigan with Resistance to Stripe Rust

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