2004
DOI: 10.1016/j.enzmictec.2003.11.015
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Production and partial characterization of thermophilic proteases from Streptomyces sp. isolated from Brazilian cerrado soil

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Cited by 50 publications
(26 citation statements)
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“…Shaking at 100 rpm markedly increase proteases production by S. aburaviensis. This finding was also reflected in other studies [24,26,28,29].…”
Section: Discussionsupporting
confidence: 78%
See 1 more Smart Citation
“…Shaking at 100 rpm markedly increase proteases production by S. aburaviensis. This finding was also reflected in other studies [24,26,28,29].…”
Section: Discussionsupporting
confidence: 78%
“…The enzymes were stored at 4°C with few drops of toluene/Sodium Azide to avoid bacterial contamination. Protease assay was done by using the method described by Meyers and Ahearn [23], which is a modified method of Hayashi et al [24]. Briefly, 3 ml of crude enzyme, 3 ml of citrate phosphate buffer and 3 ml of 1% (w/v) casein was taken in a 25 ml test tube, and the tube was placed in a water bath at 35°C for 1 hour.…”
Section: Measurement Of Enzyme Activitymentioning
confidence: 99%
“…Activity staining was performed on 0.1% copolymerized gelatin as substrate to study protease activity, according to De Azeredo et al (4). Lipase activity staining was performed using tributyrin agar according to the method of Singh et al (22).…”
Section: Characterisation Of Enzymesmentioning
confidence: 99%
“…Out of the four temperatures tested (40, 50, 60 and 70 o C), 60 o C was found to be the optimal for maximum enzyme activity irrespective of strain. Enzymes with high thermostability are more viable and have a better efficiency (De Azeredo et al, 2004). Activity of these proteases decreased at a temperature of 50 o C. At higher temperature (70 o C), the protease activity was also lower.…”
Section: Shake Flasks Experiments -Growth Of Isolated Strainsmentioning
confidence: 99%