Sweet potato is a vital food root, rich in vitamins, minerals, and bioactive compounds. Genotypes that meet these nutritional demands are essential to combat nutrient deficiencies and promote human health. Thus, it presupposes the hypothesis that sweet potato genotypes differ in physicochemical quality and bioactive compounds when evaluated under the same environmental conditions. Thus, this study aims to evaluate the physicochemical quality and bioactive compounds in genotypes of orange-fleshed sweet potato. The experiment was conducted from April 2022 to August 2022 in the experimental area of Agronomic Engineering, located at the Academic Unit Specialized in Agricultural Sciences (UAECA) - UFRN, in Macaíba, RN, Brazil. The treatments were composed of eight genotypes, being two cultivars (BRS Amélia and Beauregard) and six accessions (Macaíba I, Macaíba II, Macaíba III, Macaíba IV, Ceará-Mirim, and Natal I) of orange-fleshed sweet potato from the UAECA teaching collection. After harvest, the following characteristics were evaluated: firmness, elasticity, hydrogen potential (pH), titratable acidity (TA), soluble solids (SS), ratio (SS/TA), Vitamin C, and the bioactive compounds: anthocyanins, flavonoids, and total carotenoids. The orange-fleshed sweet potato genotypes showed distinct characteristics for the physicochemical variables and the bioactive compounds. Due to increased pulp pH, anthocyanins were reduced in the Ceará-Mirim, Macaíba III, and Macaíba IV genotypes. The Macaíba II and Natal I genotypes stood out regarding the SS/TA ratio, flavonoids, and anthocyanins. In contrast, the Macaíba III genotype had the highest carotenoid content and great bioactive potential.