Chemical characterization of vinegars obtained from Durian wood (Durio sp.) and their termicidal activity against Coptotermes curvignathus and antifungal activity against Schizophyllum commune were evaluated. The process of pyrolysis produced wood vinegars at three distinct temperature: 350℃, 400℃, and 450℃. To determine their effectiveness against fungal growth, the vinegars were tested using a Petri dish with 1.0%, 2.0%, 3.0%, and 4.0% (v/v) against S. commune. In the experiment, termicidal activities were evaluated using a no-choice test for C. curvignathus with 3.0%, 6.0%, 9.0%, and 12.0% (v/v). The wood vinegar exhibited antitermitic activity to C. curvignathus workers in the no-choice experiment; For vinegar produced at 450℃, a 6% concentration was required to achieve 100% mortality against C. curvignathus. In addition, a 12% vinegar produced at 450℃ resulted in the lowest mass loss of treated filter paper, which was 20.00%. Furthermore, all the wood vinegars exhibited antifungal activities against S. commune at concentration of 2.0%. The dominant chemical components of wood vinegar produced at temperature of 350℃, 400℃, and 450℃ were 2-methoxy-phenol, 4-ethyl-2-methoxy-phenol, 4-ethyl-2-methoxy-phenol, 3.5-dimethoxy-4-hydroxytoluene, and creosol.