Production and purification of a novel exopolysaccharide from lactic acid bacterium Streptococcus phocae PI80 and its functional characteristics activity in vitro

Kanmani, Paulraj; Kumar, Rakshak; Yuvaraj, N.; Paari, K. A.; Pattukumar, V.; Arul, Venkatesan

doi:10.1016/j.biortech.2010.12.118

Cited by 259 publications

(89 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chemical analyses of purified MBF-UFH showed that it is a glycoprotein composed of polysaccharide (76%) and protein (14%). The observed thermal stability is an indication of MBF-UFH predominantly composed of a polysaccharide [42,44]. The decrease in flocculating activity noticed at 100 o C might be due to the denaturation of the protein component of MBF-UFH [11].…”

Section: Effect Of Temperature On Mbf-ufh Flocculating Activitymentioning

confidence: 85%

See 1 more Smart Citation

Studies on Bioflocculant Production by Bacillus sp. AEMREG7

Okaiyeto¹,

Nwodo²,

Okoli³

et al. 2016

Pol. J. Environ. Stud.

View full text Add to dashboard Cite

Section: Effect Of Temperature On Mbf-ufh Flocculating Activitymentioning

confidence: 85%

“…The production of bioflocculant was optimal in the medium where ethanol was used as a carbon source by Pseudomonas aeruginosa [43]. Furthermore, among other carbon sources evaluated, lactose was most favourable for the production of exopolysaccharide by lactic acid bacteria Streptococcus phocae PI80 [44].…”

Section: Effect Of Carbon Source On Mbf-ufh Productionmentioning

confidence: 99%

Studies on Bioflocculant Production by Bacillus sp. AEMREG7

Okaiyeto¹,

Nwodo²,

Okoli³

et al. 2016

Pol. J. Environ. Stud.

View full text Add to dashboard Cite

“…Cellulose similarly plays a relatively minor role in pellicle produc- tion by Erwinia chrysanthemi (54). Some bacteria incorporate arabinose or a derivative into their EPS, including Vibrio parahaemolyticus (55), Acinetobacter junii (56), and Streptococcus phocae (57). Whether V. fischeri does this as well remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

Arabinose Induces Pellicle Formation by Vibrio fischeri

Visick

Quirke

McEwen

2013

Appl Environ Microbiol

View full text Add to dashboard Cite

Biofilms are multicellular communities of bacteria attached to a surface and embedded in a protective matrix. In many cases, the signals that induce biofilm formation are unknown. Here, we report that biofilm formation by the marine bacterium Vibrio fischeri can be induced by the addition of arabinose to LBS (Luria-Bertani-salt), a tryptone-based medium. Growth of cells in the presence of 0.2% arabinose, but not other sugars, induced the production of a pellicle at the air/liquid interfaces of static cultures. V. fischeri failed to grow on arabinose as the sole carbon source, suggesting that pellicle production did not occur as a result of increased growth, but experiments using the acid/base indicator phenol red suggested that V. fischeri may partially metabolize arabinose. Pellicle production was independent of the syp polysaccharide locus but was altered upon disruption of the bcs cellulose locus. Through a screen for mutants defective for pellicle production, we found that loss of motility disrupted the formation of the arabinose-induced pellicle. Among the ϳ20 mutants that retained motility were strains with insertions in a putative msh pilus locus and a strain with a defect in yidK, which is involved in galactose catabolism. Mutants with the msh gene disrupted grew poorly in the presence of arabinose, while the yidK mutant appeared to be "blind" to the presence of arabinose. Finally, arabinose impaired symbiotic colonization by V. fischeri. This work thus identifies a novel signal and new pathways involved in control of biofilm formation by V. fischeri.

show abstract

“…2). The EPS sample showed a wide absorption peak around 3200-3400 cm -1 , indicating typical hydroxyl groups (O-H) of polysaccharides suggesting that the analysed sample is a polysaccharide [12] . The peak from 2800 to 2950 cm -1 showed a weak C-H stretching frequency for exopolysaccharide of S. thermophiles ED1 [13] .…”

Section: Structural Analysis Of Eps By Ftir Analysismentioning

confidence: 96%

Yoğurttan Ekzopolisakkarit Üreten Streptococcus thermophilus Suşunun İzolasyonu ve Tanımlanması

Dertli¹

2015

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Production of exopolysaccharides (EPS) by starter Lactic acid bacteria (LAB) gained special interest due to technological role of these natural polymers. Yogurt samples were collected from different households in Turkey and bacterial strains were isolated and evaluated for EPS production. Strains potentially producing EPS were then identified to species level with 16S rRNA sequencing. An EPS producer Streptecoccus thermophilus strain was identified from yogurt samples. EPS was extracted from culture supernatants of S. thermophilus and partially purified and structural analysis of the crude EPS by FTIR spectroscopy revealed the presence of typical functional groups related to exopolysaccharides. This study explains the identification of a potential starter culture for yogurt production with EPS production. Keywords: Exopolysaccharides, Streptococcus thermophilus, yogurt, 16S rRNA, FTIR Yoğurttan Ekzopolisakkarit Üreten Streptococcus thermophilus Suşunun İzolasyonu ve Tanımlanması ÖzetStarter Laktik Asit Bakterileri (LAB) tarafından ekzopolisakkarit (EPS) üretimi bu doğal polimerlerin teknolojik rollerinden dolayı son yıllarda önem kazanmıştır. Bu çalışmada yoğurt örnekleri yerel kaynaklardan toplanmış, bakteriler izole edilmiş ve bu türlerin EPS üretimi test edilmiştir. EPS üretim potansiyeli gösteren suşlar 16S rRNA sekanslama tekniği ile suş seviyesine kadar tanımlanmıştır. Bu çalışmalar neticesinde EPS üretimi gösteren bir Streptecoccus thermophilus suşu tanımlanmıştır. EPS S. thermophilus'un kültür süpernatantından ekstrakte edilmiş, kısmi olarak saflaştırılmış ve FTIR spektreskopi ile gerçekleştirilen yapısal analiz ekstraktın ekzopolisakkaritler ile alakalı tipik fonksiyonel grupları içerdiğini göstermiştir. Bu çalışma yoğurt üretimi için EPS üretme kabiliyetinde olan potansiyel bir starter kültürün tanımlanmasını açıklamaktadır.

show abstract

Production and purification of a novel exopolysaccharide from lactic acid bacterium Streptococcus phocae PI80 and its functional characteristics activity in vitro

Cited by 259 publications

References 31 publications

Studies on Bioflocculant Production by Bacillus sp. AEMREG7

Studies on Bioflocculant Production by Bacillus sp. AEMREG7

Arabinose Induces Pellicle Formation by Vibrio fischeri

Yoğurttan Ekzopolisakkarit Üreten Streptococcus thermophilus Suşunun İzolasyonu ve Tanımlanması

Contact Info

Product

Resources

About