Production and purification of an Endo–1,4-beta-Xylanase from Humicola grisea var. thermoidea by electroelution

Abstract

Humicola grisea var. thermoidea produces two forms of extracellular xylanase. The component form 1 was purified using the electroelution method, due to the very small production of this extracellular enzyme. The apparent molecular mass was 61.8 kDa by SDS-PAGE.

Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation

“…The ion exchange resins DEAE-Trisacryl and S-Sepharose were also used with differente buffers and pHs, however, xylanase did not link to any of them under the assay conditions, suggesting that this enzyme was a glycoprotein (Boyer, 1993). The purification of this enzyme could be done through electroelution technique, used by Monti et al (2003) to purify the form I xylanase from Humicola grisea var. thermoidea.…”

Liquefação de farelo de trigo para produção de xilanases por cultivo submerso de Aspergillus niger

“…Esta etapa de desramificação inicia a hidrólise enzimática removendo os bloqueios para clivagem da cadeia principal de xilana.Além das enzimas xilanases(HARRIS et al, 1997;MONTI et al, 2003), o A. niger produz pectinases, proteases, α-amilase, glucomilase, celulase, ácidos orgânicos e proteínas recombinantes(WARD et al, 2005). No entanto, há um grande gasto energético do fungo para a transcrição e regulação dos genes codificadores das enzimas.…”