Production and purification of syringomycin, a phytotoxin produced by Pseudomonas syringae

Characterization of the Transcriptional Activators SalA and SyrF, Which Are Required for Syringomycin and Syringopeptin Production byPseudomonas syringaepv. syringae

“…For microarray analysis, P. syringae pv. syringae strains were cultured on syringomycin minimal medium with exogenously added arbutin (100 M) and D-fructose (0.1%) (SRM AF ) (23). For GUS assay experiments, P. syringae pv.…”

The structure of syringomycins A1, E and G

“…The first paper on SR production by pathogenic isolates of P. syringae [2] was followed by a wide interest in the role of the toxin in plant pathogenesis, but surprisingly this has not been paralleled by studies on its structure. In fact, until recently, the only available information was derived from preCorrespondence address: A. Ballio, Dipartimento di Scienze Biochimiche, Universit/l di Roma 'La Sapienza', Piazzale A. Morn, 5, 1-00185 Roma, Italy liminary analyses carried out on preparations which appeared homogeneous by disc electrophoresis [5,6]; they showed that SR was a low molecular mass, peptide-containing substance which on acid hydrolysis yielded Arg, Phe, Set and an unidentified basic amino acid in a 1:1:2:2 molar ratio [6]. More recently, it has been reported that the above preparations are mixtures which can be resolved by reverse-phase HPLC into a number of components, all containing Arg, Phe, Ser and Dab in the molar ratio 1:1:2:2, as well as some other unidentified amino acids and a long chain 3-hydroxy fatty acid [7].…”

“…syringae is a bacterial pathogen of numerous monocot and dicot plants [1]. Most strains of this organism produce a nonhost-specific toxin called syringomycin (SR) which significantly contributes to virulence [2][3][4]. The first paper on SR production by pathogenic isolates of P. syringae [2] was followed by a wide interest in the role of the toxin in plant pathogenesis, but surprisingly this has not been paralleled by studies on its structure.…”

of Some New Heterocyclic Schiff Bases Derived from Thiocarbohydrazide

“…The standardized disc agar diffusion method 15 was followed to determine the activity of the synthesized compounds against the sensitive organisms Staphylococcus -23 19 8 21 15 21 16 21 18 17 10 20 14 9 --21 15 13 10 --27 24 11 --8 7 --16 13 --12 --18 15 ------13 16 10 9 7 ----19 15 14 --------16 12 15 16 13 18 16 18 14 --28 24 16 17 13 8 7 ----23 17 Control # 35 26 35 25 36 28 38 27 35 The compounds were dissolved in DMSO which has no inhibition activity to obtain concentration of 100 μg mL -1 . The test was performed on medium potato dextrose agars (PDA) which contains infusion of 200 g potatoes, 6 g dextrose and 15 g agar.…”